StandardCellTrace™VioletTCellProcedure

实验概要The CellTrace™ Violet Cell Proliferation Kit provides a versatile and well-retained cell tracing reagent in a convenient and easy-to-use form. The kit contains CellTrace™ Violet in nine single-use vials to permit small scale experiments without preparing excess quantities of stock solution. CellTrace™ Violet easily diffuses into cells where it is cleaved by intracellular estera......阅读全文

CellTrace™-CFSE-Cell-Proliferation-Kit

实验概要The CellTrace™  CFSE Cell Proliferation Kit provides a versatile and well-retained  cell-tracing reagent in a convenient and easy-to-use form. The

Standard-CellTrace™-Violet-TCell-Procedure

实验概要The CellTrace™  Violet Cell Proliferation Kit provides a versatile and well-retained  cell tracing reagent in a convenient and easy-to-use form. T

流式细胞术数据分析知识汇总(五)

第四章 FCM数据分析之增殖、周期、凋亡检测1、细胞增殖检测CellTrace细胞增殖试剂盒含有细胞膜通透性非荧光酯,可通过扩散透过细胞膜进入细胞,并与胺基反应形成荧光分子。这种无荧光的酯进入细胞后,可被细胞内酯酶转化为荧光产物。活化的琥珀亚胺酯与蛋白质中的胺基基团共价结合,使染料能够长时间保留在细

光片照明显微镜应用于炎症性肠道疾病研究

文献简述——Three-Dimensional Cross-Sectional Light-Sheet Microscopy Imaging of the Inflamed Mouse Gut 前言:目前,一些炎症性肠道疾病(IBD),如克罗恩病(CD)或溃疡性结肠炎(UC)等,其发病的确切机理仍然

LIVE/DEAD®-Violet-Viability/Vitality-Kit

实验概要The  LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color  fluorescence cell viability and vitality assay that is based on the  simultane

用CRISPR/Cas9对CART细胞进行多重基因编辑(一)

作者:Xiaojuan Liu1, *, Yongping Zhang2, *, Chen Cheng1, 3, *, Albert W Cheng4, Xingying Zhang1, 5, Na Li1, Changqing Xia2, 6, Xiaofei Wei7, Xiang Li

用CRISPR/Cas9对CART细胞进行多重基因编辑(三)

流式细胞术 Flow cytometry CytoFLEX (Beckman Coulter Inc) was used to perform fluorescent expression analysis. Cells were harvested on the following day