porcinebraintubulinprep
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have done. The protocol calls for 3 pig brains, and should yield ~ 60 mg of purified tubulin. Solutions and reagents: 100g P-11 cellulose phosphate fibrous cation exchanger (Whatman Inc, Clifton NJ) 6L 0.1M HCl 6L 0.1 M NaOH 2L 0.1 M MgSO4 2L ......阅读全文
BrainX：3D打印全尺寸人脑模型及临床应用前景
目前，手术仍然是治疗脑部肿瘤以及多种原因造成脑外伤的有效手段之一。但由于人类大脑的复杂结构，极大提高了手术过程的难度。因此，根据患者病情而定制的术前大脑模型能够帮助外科医生精准分析病灶和提高手术熟练度，从而提升手术成功率。然而，基于已有的模具成型方法，难以根据患者的大脑，“打印”出高精准度的大脑模型
Brain：遗传变异与精神分裂症的分子机制联系
精神分裂症（Schizophrenia）是一组病因未明的慢性精神疾病，临床上往往表现为症状各异的综合征，涉及感知觉、思维、情感和行为等多方面的障碍以及精神活动的不协调。基于双生子的遗传学研究显示精神分裂症遗传率约79~81%,表明遗传因素在精神分裂症中具有重要作用。 目前，国际上已开展一系列大
TUBB基因编码的功能和结构描述
这个基因编码一个β-微管蛋白。这种蛋白质与α-微管蛋白形成二聚体，并作为微管的结构成分。该基因突变导致皮质发育不良，复杂，伴有其他脑畸形6。选择性剪接导致多个剪接变体。这个基因在1、6、7、8、9和13号染色体上有多个假基因。This gene encodes a beta tubulin prot
TUBB基因突变因子与药物介绍
这个基因编码一个β-微管蛋白。这种蛋白质与α-微管蛋白形成二聚体，并作为微管的结构成分。该基因突变导致皮质发育不良，复杂，伴有其他脑畸形6。选择性剪接导致多个剪接变体。这个基因在1、6、7、8、9和13号染色体上有多个假基因。[由RefSeq提供，2014年6月]This gene encodes
美BRAIN计划发布首个技术成果：无创技术启动和关闭神经元
研究人员开发了一种新化学遗传学（chemogenetic）技术，可以通过启动和关闭神经元，揭示控制小鼠行为的大脑回路。这是美国NIH BRAIN计划产出的首个技术成果，可以帮助人们调节神经元进而治疗疾病。 北卡罗来纳大学和NIH的研究团队在四月三十日的Neuron杂志上发表文章介绍了这一成果。
Preparation－of－tubulin2
DAY 2: Cycling preparation of MT protein.Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport
细胞组分和细胞器——细胞骨架
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin and Quantifying Labeling Stoi
Tubulin－Preparat
Materials3 - 5 Fresh Pig Brains1 M GTP1 M Magnesium SulfatePM buffer =100 mM Pipes, pH 6.9 2 mM EGTA 1 mM Magnesium Sulfate2 mM DTTPM-4M Buffer =100 m
Recycling－Tubulin
Recycling TubulinWe "recycle" tubulin fractions stored at -80¡C after the PC column and store the recycled tubulin in small aliquots for day-to-day us
Tubulin－Basics
I. Useful Values1 mg/ml tubulin = 10 µM (assuming MW of ab-tubulin heterodimer is 100,000; in reality it is ~110,000 but almost all tubulin labs use t
Tubulin－Basics
I. Useful Values1 mg/ml tubulin = 10 µM (assuming MW of ab-tubulin heterodimer is 100,000; in reality it is ~110,000 but almost all tubulin labs use t
Preparation－of－tubulin
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have d
Isolation－and－culture－of－porcine－muller－cells
1. Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-co
Immunofluorescent－Localization－of－Tubulin
LEVEL IIMaterialsCoverslip cultures of an appropriate monolayer cell linePhosphate buffered saline (PBS)Acetone/Methanol (absolute) in a 50:50 volume
TRIzol－Prep
Procedure1. Homogenize cells (10 million) or tissue (50-100 mg) in 1 mL TRIzol Reagent (e.g. scrape and pass through 30G needle, dounce homogenize an
Brain：科学家识别出参与阿尔兹海默病中神经元易感性发生的关键基因
神经变性疾病早期阶段的特征是离散脑细胞群中蛋白质的积累以及这些脑细胞的退化，对于大多数疾病而言，这种选择性的易感性模式是无法解释的，但其对于病理性机制或许能提供重要的见解。阿尔兹海默病是世界上主要的痴呆症类型，其特点是会表现出两种标志性的病理性病变，即淀粉样斑块（肽类的细胞外聚集）和神经原纤维缠
Studier－Lysate－Prep
SummaryHow to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep.Pro
CHO－Centrosome－Prep
CHO Centrosome Prep:Arshad Desai4/94Cells:We grow our CHOs with MEM[[alpha]] (without nucleosides) + 10% Bovine Calf Serum and penn/strep/glutamine. F
Yeast－DNA－Prep
Protocolgrow up yeast culture to appropriate density (near saturation)spin 1.5 mls of culture for 1 min in microfuge and aspirate off supernatantresus
SDS－Gel－Electrophoresis－of－Tubulin\MAPs
MaterialsStock Acrylamide: (30%T:0.8%C)30% by weight of acrylamide0.8% by weight of N,N'-bis-methylene acrylamideSeparation Gel (Final Concentrati
Tubulin－Polymerization－with－GTP/GMPCPP/Taxol
I. Solutions & SuppliesII. Prepolymerization ClarificationIII. GTP PolymerizationIV. Taxol PolymerizationV. GMPCPP PolymerizationVI. Determining Conce
Large－Scale－Tubulin－Preparation——2
III. Pouring a 1L Phosphocellulose (PC) ColumnResin: Whatman P11 Cellulose Phosphate -- fibrous cation exchanger(1 gram of PC swells to about 4 ml pac
Labeling－Tubulin－and－Quantifying－Labeling－Stoichiometry
Labeling Tubulin and Quantifying Labeling StoichiometryThis is a general procedure for coupling moieties with reactive succinimidyl esters to tubulin.
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton and Larysa H. Pevny1UNC Neuroscience Center
Streptomyces:Protocols/Spore－Prep
Spore Prep - Inoculating & HarvestingDescription A spore prep is a method of preserving a sporulating strain of Streptomyces. The stock is stored in 2
Competent－agro－prep－for－electroporation
day 11. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr, Koncz & Schell) in YEP in 250 mL baffle flasks.2. Grow at 28 °
Yeast－Genomic－DNA－Prep
Grow 10ml YPD cultures o/n. Figure out cell density; inoculate 30 ml YPD and grow o/n so that cell density is approximately 2 x 108cells/ml the next m
活细胞成像在中枢神经系统（CNS）疾病和紊乱研...（三）
Huvec细胞： Huvec细胞（固定），用SiR-actin染色，共聚焦显微镜成像。 大鼠海马神经元： 用SiR-actin染色培养大鼠海马神经元的STED图像。肌动蛋白环（条纹）周期性为180nm。 MCF10A Cells
Extraction－of－Chromatin
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.3 - Extraction of ChromatinLEVEL IIMaterials Bovine or porcine bra
Tissue－Harvest－Protocol
TISSUES TO BE PROCURED(minimally and preferably within 5-8 hours after death):1. Brain2. Liver3. Muscle4. Skin5: Others as the specific case dictatesP