StudierLysatePrep
SummaryHow to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep.ProtocolAdd 5mL fresh overnight (BL21 if wild-type T7) into 15 mL of T broth (125 mL flask).I often use LB --Sri Kosuri 15:18, 3 Jun 2005 (EDT)Add a single plaque to above (or a drop of lysate)Shake at 30°C until lysis is visually apparent (2-3hr)As soon as lysis is observ......阅读全文
Studier－Lysate－Prep
Summary How to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep.
Silver:－Lysate－for－Western
Grow cellsHarvestSpin downWash with PBSSpin down enough cells to collect a 50-100 µL cell pellet in a FastPrep tubePrepare lysis buffer (beforehand) a
Cesium－Chloride－Purification－of－T7
SummaryCleaner stocks of T7 that concentrates and purifies T7 bacteriophage.ProtocolGrow 100mL of permissive cells to a density of 108 to 109 cells/ml
TRIzol－Prep
Procedure1. Homogenize cells (10 million) or tissue (50-100 mg) in 1 mL TRIzol Reagent (e.g. scrape and pass through 30G needle, dounce homogenize an
CHO－Centrosome－Prep
CHO Centrosome Prep:Arshad Desai4/94Cells:We grow our CHOs with MEM[[alpha]] (without nucleosides) + 10% Bovine Calf Serum and penn/strep/glutamine. F
Yeast－DNA－Prep
Protocolgrow up yeast culture to appropriate density (near saturation)spin 1.5 mls of culture for 1 min in microfuge and aspirate off supernatantresus
Competent－agro－prep－for－electroporation
day 1 1. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr, Koncz & Schell) in YEP in 250 mL baffle flasks. 2. Grow at
Yeast－Genomic－DNA－Prep
Grow 10ml YPD cultures o/n. Figure out cell density; inoculate 30 ml YPD and grow o/n so that cell density is approximately 2 x 108cells/ml the next
Streptomyces:Protocols/Spore－Prep
Spore Prep - Inoculating & Harvesting Description A spore prep is a method of preserving a sporulating strain of Streptomyces. The stock is stored
porcine－brain－tubulin－prep
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have d
质粒的大量制备
· Plasmid Mini and Maxi Prep Methods (Gimila Lab) · Maxi-preps and all media, solutions (NWFSC)Isolation of cosmid, plasmid and P1 DNA
质粒的大量制备
· Plasmid Mini and Maxi Prep Methods (Gimila Lab) · Maxi-preps and all media, solutions (NWFSC)Isolation of cosmid, plasmid and P1 DNA
CSF－Extract－Prep－for－Spindle－Assembly
This protocol is essentially as described by Murray (1991), Cell Cycle Extracts. In Methods in Cell Biology, B.K. Kay and B. Peng, eds. (San Diego: A
Acid－Phenol－Yeast－RNA－Prep
This is the preferred method for yeast RNA preparationuse Gloves and RNAse free solutions throughout.1. Use a YPD overnight culture to innoculate fres
Streptomyces:Protocols/MiniMaxi－Prep
Small Scale Plasmid Isolation (Mini / Maxi Prep)Description A mini prep / maxi prep is used to isolate plasmid or cosmid DNA from bacteria, normally E
细菌人工染色体
The Construction of Bacterial Artificial Chromosome (BAC) Libraries (complete manuscript) (Clemson University Genomics Institute) Construction of BAC
质粒的小量制备
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip
质粒的小量制备
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip
TritonPrep－Method－for－bacterial－DNA－Purification
Triton-Prep Method for bacterial DNA PurificationGrow 5 ( large scale-15ml culture). Harvest in single eppendorf tube (or 15 ml disposable tube).Resus
重组DNA的分离、克隆与测序实验手册8
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
A－quick－RNA－miniprep－for－Neurospora－mycelial－cultures
Most RNA isolation techniques currently in use have been developed for the processing of large quantities of material. These typically involve multip
PREP自动酶切仪使用方法
一 开机前检查：1 检查仪器台面（DECK）上所有的实验材料（Labware）。2 检查SystemWater 水桶的水位。3 检查恒温循环水浴（Chiller）水箱的水位，并定期更换或填充Chiller 中的循环水。4 倒掉废液桶中的废液。二 开机步骤：打开Chiller、Heater、仪器及计算
Quick－Yeast－DNA－Prep:－Isolation－of－Total－DNA－(genomic－and－plasmid)
Grow a 5 ml YPD O/N culture inoculated with a single yeast colony at 30 deg. Transfer culture to a small 13 x 100 glass tube. Spin down cells 2
GSP融合蛋白的准备
GST Fusion Protein PrepItalics indicate optional steps especially useful for the analysis of untested proteins.GROWTH AND HARVESTING OF BACTERIAAdd 10
DNA纯化手册2
Key points to observe: a. Use a endA1- E. coli strain for plasmid propagation and isolation whenever possible. The instability of plasmids isolated fr
Column－Method－for－Lambda－Phage－DNA－Preparation
Purpose: Mini-prep method for lambda phage DNA purification from lysates. Time required: 4 hours once the lysate is in hand Special suppl
岛津Nexera－UC－Prep系统进入ANTOP专家评审阶段
分析测试百科网讯 炎炎夏日，火的不仅有气温，火的还有这些分析仪器。经过广大网友踊跃参与和热情投票，岛津申报的“制备超临界流体色谱创新奖”已进入专家评审阶段！岛津Nexera UC Prep半制备超临界流体色谱系统 岛津Nexera UC Prep半制备超临界流体色谱系统集合了Nexera UC
Fast－and－reliable－miniprep－RNA－extraction－from－Neurospora－crassa
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extrac
RNA－Extraction－(mini－prep):Trizol法实验原理和步骤
RNA的制备与分析对于了解基因在转录水平上的表达与调控和cDNA的合成都是必须的，RNA的纯度和完整性对于Northern blot，RT-PCR 和cDNA文库的构建等分子生物学实验都至关重要。RNA分离的方法很多，其中最关键的因素是尽量减少RNA酶的污染 实验原理： Trizol 试剂
通过Pippin－Prep和Blue－Pippin制备凝胶电泳，可以增加基因...
通过Pippin Prep和Blue Pippin制备凝胶电泳，可以增加基因、转录起始位点和剪切位点的鉴定通过Pippin Prep和Blue Pippin全自动制备凝胶电泳系统，可以增加基因、转录起始位点和剪切位点的鉴定环亚生物科技有限公司（APG BIO Ltd）是美国Sage Science公