DynamicFlowAssayinaParallelPlateFlowChamber
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of cell adhesion under well-defined wall shear stress. The visualization of the different events of cell adhesion can be quantified by selective image acquisition and subsequent image processing. Flow assays are uniquely suited to the investigation of adhesive events which ......阅读全文
MiniChamber－for－Regulating－Gaseous－Environment－During－Culture
Mini-Chamber for Regulating Gaseous Environment During Culture R.M. Rivera and P.J. HansenA simple and inexpensive chamber for regulating gaseous envi
如何借助epMotion－5073l移液工作站完成微量体积的...（二）
Figure 1: epMotion 5073l worktable for qPCR setup Results and DiscussionThe automation of the qPCR setup using the 10 µL dispensing tool and the epM
TUNEL－assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Aspartate－Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Phosphate－Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
Protease－assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to softe
Polygalacturonase－assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
Protease－assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in
MTT－Assay
This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in
Bradford－Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
DGK－Assay
Buffers:- 2X buffer10 ml 0.5 M imidazol, pH 6.60.21 g LiCl1.25 ml 1 M MgCl21.0 ml 0.1 M EGTA, pH 6.6--> Bring volume up to 50 ml with distilled water.
Motility－Assay
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
Pectinase－assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are w
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
什么是－Flow－Chemistry－？
flow chemistry 一般译作 流动化学。本意指在连续流动的系统中完成化学反应，不同于批次式反应。其实流动过程中完成化学转化的生产方式并不独特，早已广泛用于石化工业和 合成氨、硫酸、盐酸、硝酸等大化工领域。真正让流动化学独具魅力的是 小型化 和 智能化。流动化学不仅仅单纯指物料的流动，而是结
ProcartaPlex－免疫检测常见问题及解决方案
eBioscience公司Luminex液相蛋白芯片检测试剂——ProcartaPlex可以为您的生物学研究提供各种多因子检测方案，包括细胞因子、生长因子、趋化因子和其他蛋白。在选择试剂盒及操作时会遇到很多问题，现将常见问题及答复汇总如下，供您参考： 如果不小心将整个试剂盒保存在-20°C. 这个试
MiniChamber－for－Regulating－Gaseous－Environment－During－Culture
1. The basic unit is constructed from a media filtration unit such as Corning’s 115 ml filter system (catalog numbers 430944-430947).2. Tygon tubing (
双通道尤斯灌流室（Ussing－Chamber）
UC-2M双通道尤斯室（尤斯灌流室）基座用于安装各种规格的尤斯室。尤斯室（尤斯灌流室）及其基座共同组成离体组织体外实验装置。将上皮组织安装在尤斯室中，尤斯室内加入生理溶液，并利用基座恒温和通氧，可使离体的上皮组织在较长时间内继续保持生理活性，以便研究者对上皮功能进行体外研究。 UC-2M是
双通道尤斯灌流室（Ussing－Chamber）
UC-2M双通道尤斯室（尤斯灌流室）基座用于安装各种规格的尤斯室。尤斯室（尤斯灌流室）及其基座共同组成离体组织体外实验装置。将上皮组织安装在尤斯室中，尤斯室内加入生理溶液，并利用基座恒温和通氧，可使离体的上皮组织在较长时间内继续保持生理活性，以便研究者对上皮功能进行体外研究。 UC-2M是
Neutralizing－Bioassay－Protocols
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
信号传导
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary c
信号传导
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary c
RAT/MOUSE－GROWTH－HO...
实验概要This Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive quantification of Growth Hormone in rat or mouse serum, plasma, tissue
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells2
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution 1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well of an eight-we
Immunostaining－Thin－Layer－Chromatograms－Of－Glycolipids
Immunostaining Thin Layer Chromatograms Of GlycolipidsJohn L. Magnani~GlycoTech Corporation, Rockville, Maryland 20850Immunostaining thin layer chroma
Marcantonio－Lab－Protocol－Manual
Protein GelPreparing and Running a Protein Gel (7% Polyacrylamide) A. Preparation of Running Gel Solution 1) Add to a 50 ml cylinder:DD-H2O 26 ml30% a
The－UnderAgarose－Migration－Assay2
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the best magni