96WellSamplePreparationforSuspensionCells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.). In this procedure, cells are plated into the wells of 96-well filter bottom plates and stimulated as desired. At the end of the stimulation, cell culture medium is removed from the bottom of the wells by gentle aspiration using a vac......阅读全文
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96well－Plate－Dual－Luciferase－Assay96孔板荧光素酶检测
Reagents: Plasmids: can be found in my plasmid(1) box in the ?20oC freezer. 985 : FR-tk-luciferase 2517 : Renilla-tk-luciferase 2145 : GFP Lipfectami
Isolation－Of－PCR－Products
实验概要Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents.实验原理The ChargeSwitch® TechnologyT
Automated－Genomic－DNA－Extraction
实验概要This section provides a general protocol for automated isolation of genomic DNA from 10-20 µl blood samples in a 96-well format using the Charge
High－Throughput－Isolation－Of－PCR－Products－Using－ChargeSwitch®－PCR－CleanUp
实验概要The ChargeSwitch® PCR Clean-Up Kit allows rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic aci
Automated－Extraction－－Normalized－DNA－Buccal－Kit
实验概要This section provides a general protocol for automated isolation of genomic DNA from human buccal cell swabs in a 96-well format using the Charg
Measurement－of－Cell－Adhesion－Under－Static－Conditions
Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell adh
NKcell－cytotoxicity－assay
Outline:To measure NK cell killing, suitable target cells are labeled with 51Cr, washed and incubated together with the killer cells (and treatments).
The－E.Z.N.A.®－MagBind™－Dye－terminator－Removal－Procedure
实验概要Excess unincorporated, nonradioactive label can cause high background fluorescence in automated sequencing gels. For optimal sequencing results
Colony－PCR－Protocol
1. Pull out eight glycerol stock plates from the –80oC freezer and set on bench top to thaw. Be sure to remove the foil seal before leaving the plates
Cellbased－ELISA－for－primary－cells
1. The 96-well micro-plates are pre-coated with extra cellular matrix.2. Primary cells are grown in the 96-well plates. Seed 100μl of 10,000-20,000
Neutralizing－Bioassay－Protocols
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
Chemotaxis－Assay趋化性实验
Springer Lab，The CBR Institute for Biomedical Research, Inc. Department of Pathology Harvard Medical Schoolhttp://cbr.med.harvard.edu/investigators/sp
Chemotaxis－Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
Transposonmediated－Mutagenesis
Transposon-mediated MutagenesisStep 1 - Amplify ORF from MG16551.0 ul genomic DNA (30ng/ul)5.0 ul gene specific primer mix4.0
Cytokine－Bioassays
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
竞争性分析Epitope－Mapping实验方法
ABSTRACTThe simplest way to determine whether two monoclonal antibodies bind to distinct sites on a protein antigen is to carry out a competition assa
Cr－Release－Cytotoxicity－assay
DescriptionCytotoxic activities of mNK cells are examined in 51Cr release assay from target cells ProcedureEffector cells (mNK cells) are seeded into
Apricot－Designs－PP5+1应用实验案例（二）
MethodsFigure 2. Predicting Systemic Clearance from In Vitro DataConclusionsA simplified 2-point, higher throughput 96-well assay can be used to ranka
如何借助epMotion－5073l移液工作站完成微量体积的...（一）
如何借助epMotion 5073l移液工作站完成微量体积的qPCR反应体系构建在进行微量体系的液体操作时，如何避免人为误差与日间变化，如何保证结果的一致性提高实验效率？如果减少冗长枯燥的重复操作，减少昂贵试剂的使用，提升操作体验节省实验经费？本篇应用旨在介绍如何用10 μL分液工具实现全自动的微量
Use－of－the－Bradford－Protein－Assay－in－a－Microtiter－Plate－Format
Introduction The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in
细胞培养——细胞生长和细胞毒性
Articles posted in the Method Froum Cell Viability AssayDye exclusion method Viable Cell Counts Using Trypan Blue (Gibco) Soft Agar Assay For Colo
Apricot－Designs－PP5+1应用实验案例（一）
PERSONAL PIPETTOR全自动移液工作站是Apricot Designs研发生产的集多种移液功能于一身的紧凑型全自动移液工作站，具有高效、便捷、精准的特性，能同时兼容1/8/12/16/24/96/384通道的液体处理，开放式的设计可以便于整合叠板机和机械臂等。特点和优势集多种移液功能
Isolation－and－Culture－of－Human－Brain－Tumor－Stem－Cell
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
Fusion－and－Cloning
ReagentsMedium A - Pre-fusion Medium and Hybridoma Expansion MediumMedium B - Fusion Medium Medium C - Hybridoma Recovery MediumMedium D - Hybridoma S
Fusion－and－Cloning
Author: Nanci DonackiSource: Contributed by Nanci DonackiAbstract: Procedure for establishing hybridoma in one stepReagents(StemCell Technologies, Inc