WIKI资讯
WIKI资讯
Purpose:Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extraction.Time required:15-20 minutes to begin growing 2-4 cryovialsProcedure:Frozen cells should be thawed quickly. Remove the cryovial from its long term storage container in the -135 degrees C Cryostar, and place immediately in a 37 degrees C water bath for 2 minutes.Rem......阅读全文
Method: Preparation of Lymphoblastoid Cell Lines for Long Term StorageMay 30, 1990Rosalie VeilePurpose:To store cell lines in a form that will insure