StandardRTPCR
RT-PCR or reverse transcription PCR refers to PCR that uses product of an RT reaction as template. In effect, the PCR amplifies cDNA fragments. In one-tube RT-PCR, RNA and PCR primers are added to a reaction mix that is thermocycled for RT first followed by for PCR. One-tube RT-PCR reaction mixes are supplied by many manufacturers. Drawbacks to their use include lack of flexibility. Otherwise, RT-PCR is a t......阅读全文
Standard RT-PCR
RT-PCR or reverse transcription PCR refers to PCR that uses product of an RT reaction as template. In effect, the PCR amplifies cDNA fragments. In one
Standard PCR reaction
Steps for Standard PCR ReactionDesign primers. In general, primers should have the following properties:Tip: Primer3 is an excellent resource for choo
Standard neutral agarose electrophoresis
Standard neutral agarose electrophoresis Standard agarose gels can be prepared using either TBE or TAE running buffers. You will need: Either 10 x
Thermal Cycling Profile for Standard PCR
Initial denaturationIt is very important to denature the template DNA completely. Initial heating of the PCR mixture for 2 minutes at 94°–95°C is enou
Standard Protocols Autoradiography (35S)
Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted). Make up the developer and the fixer and p
Standard Protocol For Up to 10 ml Whole Blood
实验概要 The E.Z.N.A.® Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to 25 ml of fresh, whole blood treated with any common anti
Standard CellTrace™ Violet T-Cell Procedure
实验概要The CellTrace™ Violet Cell Proliferation Kit provides a versatile and well-retained cell tracing reagent in a convenient and easy-to-use form. T
定量RT-PCR （Quantitative RT-PCR）
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of mRNA expres
First Standard®推出9种亚硝胺混标
近日来，据央广等多家媒体报道，清华大学环境学院国家环境模拟与污染控制重点实验室陈超课题组，对全国饮用水系统中亚硝胺类消毒副产物进行普查发现，中国是世界上亚硝胺检出情况最多样的国家，其中亚硝基二甲胺（NDMA）的浓度最高。流行病学研究表明，亚硝胺与消化道癌症密切相关，它也被认为“像极了当年空气污染
Fluidigm 正式更名为 Standard BioTools Inc.
Unleashing tools to accelerate breakthroughs in human health尊敬的客户：在这激动人心的时刻，我谨代表公司宣布，Fluidigm已正式更名为Standard BioTools Inc. 这一新的名称代表着我们公司的发展愿景，通过为您提供必备的