DetectionofVirusesinInfectedPlantExtractsusingImmunocapturePCR
1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0), 138 mM NaCl, 1 mM PVP, 0.05% Tween-20, 3 mM KCl, and 3 mM NaN3 per liter (pH 7.4).PBS-Tween wash buffer: 138 mM NaCl, 1.5 mM KH2PO4, 8 mM Na2HPO4, 3 mM KCl, 0.05% Tween-20, and 3 mM NaN3, perliter pH 7.4)Antibodies2) PCR stage For a single reaction of 50......阅读全文
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