WIKI资讯
WIKI资讯
We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Designed dsRNA). It is also possible to produce dsRNA using PCR generated DNA templates containing either the T7 & SP6 or the T7 & T3 promoters on either end (II. dsRNA Fom Clones). This method is less efficient, especially when working on a large scale.All work shou......阅读全文
实验概要We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Prim
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we
THESE INSTRUCTIONS APPLY TO ORDERS CONTAINING THE FOLLOWING CELL PRODUCTSCryopreserved Cells (Single donor)CC-2511NHDF -Ad3 500,000 cells/cryovia
Materials(Solutions are all available from the media room)200ml bottle of 2x SD200ml bottle of 4% agar -- make sure to sign it out40% glucoseCSM minus
BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to ente
PCR primers should be free of significant complementarily at their 3'-termini as this favours formation of primer-dimer which reduce product yield
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
针对Elisabeth Bik发文质疑2008年发表在Blood杂志上的文章数据，曹雪涛院士本人进行了回应： Elisabeth Bik提出的质疑： Figure 1A. The "unstimulated" and "LPS" panels in t
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowl
Electrocompetent CellsFrom Danijela Dukovski at Harvard Medical School. This protocol works well. --Julie NorvilleMaterialsDI water10% GlycerolSpecial
Do you rely on tank scales when filling, batching or tracking inventory? If so, accuracy limitations can diminish product quality, leading to mate
This workbook was developed for use with Module 2 of the InVitro Insights Cell Culture Training Program, developed by Becton Dickinson. The
For direct gene transfer of tibialis anterior (TA) muscle in mice: It is optimal to use 6-8 week old mice (weight 19-21 gm). Females give better immun
1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml
GenJet Ver.II，LipoD293及PolyJet针对哺乳动物细胞的DNA体外转染试剂，均为您提供两种不同的转染步骤——一般步骤及高级步骤，这两种步骤分别针对不同的哺乳动物细胞。高级步骤主要针对难转的哺乳动物细胞，例如：MDCK，MDA-MB231，Caco-2等细胞。使用一般的转染步
RNA extraction with TRIzol (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total RNA extractio
2. Respiratory control on glutamate and inhibition of electron transportAdd 30-40 µl mitochondria, obtain a steady state. We use a larger
写这篇博文的动机 1．两位初次被邀参与函评NSFC基金项目申请的青年学者来信问我：NSFC申请书评审的标准如何把握？ 2．我有“好人为师”的老毛病。我总是认为：经历丰富的人有责任和义务向经历不足的人“传授”经验。 3．就如美国基金委被美国人认为是“美国标准下”最公正的基金机构一样，中国基金
In our teaching lab we encourage students to work with each other and to share insight, experience, and even experimental results. To facilitate such
Hemacytometer Reference Figure 15.Determine the Cell Count.a. Calculate the total cells counted in the four corner squares.1) If the to
Q. 一步法Instant ELISA试剂盒，能做多少个检测？How many tests can I perform with an Instant ELISA Kit?Instant ELISA包含128 tests。 其中包含可以做8个点复孔标曲，共有32孔做标曲，96孔检测样本。With a
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom
Good Pipetting PracticePipetting Techniques to Boost Your PerformanceImprove your data quality with Good Pipetting Practice™ (GPP™) – METTLER TOLE
I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are require
B: GLC procedureThe GLC will be prepared for your use. Please do not attempt to turn on the GLC on your own if it is off. Do not change any of the set
MaterialsFor purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results.Do not autoclav
The development of chick embryos has been studied since Aristotle. It is one of the most intensely studied organisms. One reason for this is that ther
General InformationPhenol/chloroform extraction is an easy way to remove proteins from your nucleic acid samples and can be carried out in a manner th
实验概要Instructions are provided below for using the E-Gel®CloneWell pre-cast agarose gels with the E-Gel® iBase™ Power System. For detailed
A. Gilson Pipetmen Autopipets (and similar models)We primarily use the Gilson autopipets in the core course labs. We have four sizes identified by the