HowdoyousynthesizeyourdsRNA
We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Designed dsRNA). It is also possible to produce dsRNA using PCR generated DNA templates containing either the T7 & SP6 or the T7 & T3 promoters on either end (II. dsRNA Fom Clones). This method is less efficient, especially when working on a large scale.All work shou......阅读全文
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In our teaching lab we encourage students to work with each other and to share insight, experience, and even experimental results. To facilitate such
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5G/NR－－OTA－（二）
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Bespoke－Metal－Detection－Conveyor－Systems
Many metal detection applications do not fit into the scope of standard conveyor systems. For this reason, METTLER TOLEDO SAFELINE are able to
Recommended－Experiments－with－Isolated－Mitochondria2
2. Respiratory control on glutamate and inhibition of electron transportAdd 30-40 µl mitochondria, obtain a steady state. We use a larger volume tha
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
怀恨在心真的对机体健康有害！
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Gastrulation－Models
Gastrulation has been celled "the most important time of your life" (Lewis Wolpert); without it you would be flat like a pancake! Vertebrate gastrulat
siRNA用户手册
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et
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Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
Polyclonal－Antibody－Production
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Following PCR, you often want to get rid of the PCR primers and Taq polymerase before the next step. This is necessary for sequencing PCR products or
早期胚胎发育中的单胚胎细胞基因表达（一）
Single-embryo Gene Expression for Early Embryo DevelopmentMylene Yao, M.D. Assistant ProfessorDept. of Obstetrics and Gynecology Stanford UniversityMy
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No two cell lines behave exactly the same, so you must learn the peculiarities, or personality, of each of the cell lines with which you work. Irresp
High－Efficiency－Transformation
Day 0 Make sure you have the necessary solutions (instructions for how to make them can be found here): Single-stranded carrier DNA PEG 335
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I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are required by
Fibroblast－Cell－Systems2
Handling Precautions Normal human cells are fragile, and require special handling: Upon receipt, immediately store cryopreserved cells in liquid
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Eccles:Protein－Lysates－from－Cells－in－Culture
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The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
5G/NR－－OTA－（四）
There is another reason why defining D gets difficult. It would get more difficult for UE case. In order to correctly define D, you need to hav
Yeast－Cell－Cycle－by－Flow－Cytometry
ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4