Primer3Plus
Oligonucleotide primers are widely used in various molecular biology techniques like DNA sequencing and the polymerase chain reaction (PCR). Since a primer serves as the starting point for DNA replication, specific binding of the oligonucleotide to the target sequence on the template strand is essential for a successful experiment. The binding specificity of a primer is determined by several of its properties, like t......阅读全文
Primer3Plus
Oligonucleotide primers are widely used in various molecular biology techniques like DNA sequencing and the polymerase chain reaction (PCR). Since a p
荧光定量PCR引物的设计原则与方法
实时荧光定量PCR是一种在DNA扩增反应中，以荧光化学物质测每次聚合酶链式反应（PCR）循环后产物总量的方法。通过内参或者外参法对待测样品中的特定DNA序列进行定量分析的方法。 实时荧光定量PCR是实验室最常用的实验。研究基因在mRNA表达水平,以及验证基因敲除后下游靶基因变化情况等等。对于新