InGelDigestionofProteinsSeparatedbyPolyacrylamideGelElectrophoresis
1. Excision of protein bands (spots) from polyacrylamide gelsRinse the gloves you use with water to avoid traces of dust in your sample.Rinse the gel with water.Excise spots with clean pipette tip (f 2 mm) cutting as close to the edge of the spot as possible (to reduce the volume of ''background'' gel)Transfer gel spot into a well of a 96-microtitre plate (Costar # 3363 + 3092)2. Reduction and alkylat......阅读全文
2010全国质谱大会大会报告（一）
加拿大Alberta大学 Li, Liang(厉良)教授 来自加拿大Alberta大学的Li, Liang(厉良)教授，做了题为：“Development of LC/MS Techniques fo
Restriction－Digest
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二维聚丙烯酰胺凝胶电泳的样品制备——可溶性样品
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A－practical－method－for－the－preparation－of－total－DNA－from－filamentous－fungi
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chlori
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites九
41. Schmidt O, Theopold U, Strand M: Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 2001, 23(4):344–35
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites五
RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites一
Comprehensive identification of novel proteins and N-glycosylation sites in royal jellyLan Zhang1,2†, Bin Han1†, Rongli Li1, Xiaoshan Lu1,3, Aiying Ni
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites七
Data analysisTandem mass spectra were retrieved using Xcalibur (version 2.2, Thermo Fisher Scientific) and AnalystTF (version 1.6, AB SCIEX) softw
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites三
Note: All of the identified proteins are from Apis mellifera. Accession is the unique number given to mark the entry of a protein in the database
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites四
Figure 2 Distribution of N-glycopeptides analyzed by different enriched methods and instruments of royal jelly proteins. A is the distribution of N-
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Expression－Library－Screening－(Procaryotic)－Using－APFusion－Proteins
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Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites二
ResultsIdentified novel royal jelly proteins To expand the number of known proteins in the RJ proteome, RJ proteins were extracted and digested wit
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Engineering－BioBrick－vectors－from－BioBrick－parts/Colony－PCR
MaterialsPCR SuperMix High FidelityVF2 primer (5''-TGCCACCTGACGTCTAAGAA-3'')VR primer (5''-ATTACCGCCTTTGAGTGAGC-3'')De
Lipoprotein－Analysis－Week－2:－Electrophoresis2
Preparation of stacking gelPrepare a 7.5 ml of 3% stacking gel in a small beaker using the following amounts of appropriate reagents.Stockfinal conc.A
Marcantonio－Lab－Protocol－Manual
Protein GelPreparing and Running a Protein Gel (7% Polyacrylamide) A. Preparation of Running Gel Solution 1) Add to a 50 ml cylinder:DD-H2O 26 ml30% a
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实验原理血红蛋白电泳（hemoglobin electrophoresis）目的是检出和确认各种正常和异常的血红蛋白。根据不同的血红蛋白带有不同的电荷，等电点不同，在一定的pH缓冲液中，血红蛋白的等电点小于缓冲液的pH时带负电荷，电泳时在电场中向阳极泳动，反之，Hb带正电荷向阴极泳动。在一定电压下，
血红蛋白电泳（hemoglobin－electrophoresis）
实验原理血红蛋白电泳（hemoglobin electrophoresis）目的是检出和确认各种正常和异常的血红蛋白。根据不同的血红蛋白带有不同的电荷，等电点不同，在一定的pH缓冲液中，血红蛋白的等电点小于缓冲液的pH时带负电荷，电泳时在电场中向阳极泳动，反之，Hb带正电荷向阴极泳动。在一定电压下，
Lipoprotein－Isolation－－First－week
As insects have an open circulatory system, the hemolymph can be simply collected through an incision in the body wall. Most conveniently, you should
Phosphatidylinositol－4Kinase－and－Phosphatidylinositol－4Phosphate－5K...
Phosphatidylinositol 4-Kinase and Phosphatidylinositol 4-Phosphate 5-Kinase AssaysInositol lipid kinases are perhaps the easiest and most straightforw
Smolke:Protocols/Western
OverviewBlotting for large V5-tagged proteins in S. cerevisiaeMaterialsY-PER (Pierce)Halt EDTA-free Protease Inhibitor (Pierce)NuPAGE Novex Bis-Tris 4
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My Experience Purifying RNA from E. coliRegarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria.
使用胶做western－blot的方法
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阐述新冠病毒感染机制，推进抗病毒药物研发
最近的预印本杂志bioRxiv发表了德国波恩大学，夏里特大学，德国马克斯·普朗克精神病研究所等单位联合研究成果，阐述了SARS-CoV-2病毒可以抑制自噬。亚精胺，MK-2206，氯硝柳胺等作用于自噬的小分子化合物，可以作为抗病毒潜在治疗药物。Analysis of SARS-CoV-2-contr
Wes：阐述新冠病毒感染机制，推进抗病毒药物研发
最近的预印本杂志bioRxiv发表了德国波恩大学，夏里特大学，德国马克斯·普朗克精神病研究所等单位联合研究成果，阐述了SARS-CoV-2病毒可以抑制自噬。亚精胺，MK-2206，氯硝柳胺等作用于自噬的小分子化合物，可以作为抗病毒潜在治疗药物。Analysis of SARS-CoV-2-con
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce