BlockingcDNAarraysprintedonamineslides
Department of Crop SciencesCollege of Agricultural, Consumer, and Environmental SciencesUniversity of Illinois at Urbana-ChampaignPurpose: After spotting cDNAs onto glass slides, the slides must be treated to bind the DNAs to the coating and to denature the DNAs so they can hybridize to the fluorescently labeled probe DNA. During the blocking step the array will disappear so be sure that the bound......阅读全文
Blocking－cDNA－arrays－printed－on－amine－slides
Department of Crop SciencesCollege of Agricultural, Consumer, and Environmental SciencesUniversity of Illinois at Urbana-ChampaignPurpose: After spott
DNA微序列技术
· Protocols for Making Drosophila Arrays (Stanford U.)Detailed protocol for making arrays including PCR Amplification of cDNAs for Printing,
Blocking－with－immunizing－peptide－protocol
实验概要The method provides a blocking with immunizing peptide protocol.实验原理Non-specific binding of an antibody to proteins other than the antigen can s
Blocking－With－Immunizing－Peptide－(BL)－Protocol
实验概要Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclonal antibodi
Immunohistochemistry－on－paraffin－embedded－sections
主要试剂1. Neutral-buffered Formalin, 10% (NBF), 1 liter (Commercially available pre-prepared from many laboratory reagent suppliers).Double -distilled H
Basic－Fluorescent－in－situ－Hybridization－(FISH)
实验概要Fluorescence in situ hybridization method is a kind of physical map drawing method, use fluorescent element mark probe, to detect probe and spli
Immunohistochemistr...
实验概要Immunohistochemistry is a classic technique used for the localization of antigenic target molecules in tissue. The method exploits the princi
ImmunohistochemistyFluorescence－Protocol1
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'&
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
Silane－and－PolyLLysine－Coating－of－Microscope－Slides
1) Wash slides in Decon solution for 30 minutes.2) Wash slides in running tap water for 30 minutes.3) Wash slides in distilled water for 2 x 5 minutes
PECHamine改性棉的涂料染色工艺探讨
摘要：采用PECH-amine对棉织物改性,自制低温自交联粘合剂为固色剂,探讨其在涂料染色中的应用性能.结果表明:棉织物经PECH-amine改性后,改变了棉纤维对涂料的吸附能力,使得涂料对棉纤维的上染率和染深性提高;经自制低温自交联粘合剂交联固着,改性棉织物的染色牢度和手感均较好.探讨了棉织物
ImmunohistochemistyFluorescence－Protocol2
Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
Synthesis－and－Probing－of－Membranebound－Peptide－Arrays2
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 µl p
Synthesis－and－Probing－of－Membranebound－Peptide－Arrays1
Synthesis and Probing of Membrane-bound Peptide ArraysRonald FrankDepartment of Chemical Biology, GBF (German Research Center for Biotechnology), 3812
cDNA
· cDNA Synthesis (Crawford Lab)mRNA can be converted into DNA (copy DNA, cDNA) by annealing oligo-dT to the 3' poly-A tail that occurs on
Use－of－SemiThin－Cryosections－for－Light－Microscopy.
Use of Semi-Thin Cryosections for Light Microscopy.Semi-thin sections can be obtained from frozen blocks of cryoprotected biological material by secti
解决非特异吸附的新方法
免疫分析中，非特异的蛋白吸附常常是阻碍高灵敏度的一个重要的因素。来自中科院等单位的研究小组开发了一种新的固相载体，有望达到"0"非特异吸附。以下是他们在 Analytical Chemistry 的文章摘要。Integrated Poly(dimethysiloxane) with an Intri
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Double－immunofluore...
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies together.In order to be able to examine the co-distributi
Mapping－Protein－Distributions－on－Polytene－Chromosomes－by－Immunostaining2
12. Hold the salivary glands at the common duct with tweezers.Transfer the glands to a drop of fixing solution on a siliconizedcoverslip. 13. Incubate
PCR－Array——基因表达分析疾病和信号通路的利器（二）
RT2 Profiler PCR Array操作流程 首先将RNA样本反转录成cDNA第一链，作为PCR模板；接着将cDNA模板与合适的即用型PCR预混液混合，等体积加入到已经固定好基因特异性引物的同一个孔板的每个孔中，然后即可运行real-time PCR循环程序。RT2 Profiler PCR
MetalEnhanced－Immu...－（二）
实验步骤1. Preparation of Fluorescein-Doped PVA FilmsThe 0.5% PVA solution containing disodiumfluorescein was spin-coated on slide substrates at 3,000 rp
Dynabeads®－Antibody－Coupling－Kit
实验概要Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclonal antibodi
Bromodeoxyuridine－Immunohistochemistry
Introduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi, et a
cDNA－Libraries
cDNA LibrariesIsolation of corresponding genetic informationInstead of synthesizing a desired gene, can we used the amino acid information to directly
CDNA文库
CDNA文库（主要内容如下）· Construction of cDNA Library· Construction of Genome DNA Library· Library Screening OthersConstruction of cD
CGH－Protocols－（三）
Hybridizationreagents: labeled tumor and normal-DNA (see protocol Nick translation) salmon sperm DNA, 10 mg/ml (e.g. Promega) human Cot1 DNA, 1 mg/ml
IHC－frozen－sections...
实验概要The method provides a guideline procedure and tips for staining of frozen sections.实验步骤Frozen sections: Once mounted on APES coated slides, frozen