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COLONY HYBRIDIZATION1) CUT 4 PIECES OF 3MM WHATMAN PAPER AND PLACE EACH ONE IN A SEPARATE CONTAINER(A CAFETERIA TRAY WILL PROBABLY WORK PERFECTLY).2) SATURATE EACH OF THE 4 PIECES WITH ONE OF THE FOLLOWING SOLUTIONS:A) 10% SDSB) Denaturing Solution (.5N NaOH, 1.5M NaCl)C) Neutralizing solution (1.5M NaCl,.5M Tris pH 7.4)D) 2X SSC** DO NOT ALLOW THE FILTERS TO BECOME TOO WET BECAUSE THE COLONIES WILL SWELL AND DIFFUSE......阅读全文
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