LargeScalePlasmid,Cosmid,BAC,PAC,andFosmidDNAIsolation
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Implementation (by Feng Chen, Hau-Qin Pan and Fu Ying) Adapted from the Genome Sequencing Center, Washington Univ. ProtocolA smear of colonies of cosmid/BAC/PAC were picked and transferred into a 12X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bac......阅读全文
Isolation－of－papillary－cells
Isolation of renal papillary cells1. For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Isolation－of－murine－splenocytes
Overview In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the c
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by keeping
Isolation－of－mouse－embryos
1. Sacrifice impregnated mouse. 2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps, use another to tear the mes
Isolation－of－colonic－epithelium
实验概要 The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine. First
Isolation－of－papillary－cells
实验概要 This protocols provides a general protocol for isolation of papillary cells. 实验步骤 Isolation of renal papillary cells 1. For isolation of pa
Isolation－Of－PCR－Products
实验概要 Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents. 实验原理 The ChargeSwitch® Tech
RNA－Isolation－Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)
RNA－isolation－for－Microarray
Description RNA extraction using TRI REAGENT. This method gives ample amout of RNA.Procedure It is 3 days procedure.Day 1:1. Harvest the cells and cen
Arabidopsis－gDNA－isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
Isolation－of－liver－lymphocyte
Isolation of liver lymphocyte Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
Isolation－of－bone－marrow
(contributed by Chris Jackson (chris.jackson@bris.ac.uk))A protocol I have used to isolate rat bone marrow is: 1. Kill the rat and dissect out the fem
Nucleolar－Isolation－Protocol
We recommend that you first download and read this page as a PDF file. Using that as your guide, you can then follow the protocol below and view a Qui
Isolation－of－colonic－epithelium
The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine.First we excise t
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
mice－islet－isolation
1. Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2. Which involves cannulation of the common bile duct and
Yeast－Nuclei－Isolation
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
Poly(A)+RNA－Isolation
Eukaryotic messenger RNA (mRNA) can be separated from the other RNA species in a total RNA preparation by affinity chromatography by virtue of the
PCR的下游应用
・ Agarose Gel Electrophoresis of PCR Products(Robert H. Cruickshank)・ Agarose Gel Electrophoresis of PCR Products(Immunology Resource)
转基因——基因标靶
Gene Targeting Outline (University of Michigan Transgenic Animal Model Core)This is a brief outline of the steps necessary to produce mice with a muta
CDNA文库
CDNA文库（主要内容如下）· Construction of cDNA Library· Construction of Genome DNA Library· Library Screening OthersConstruction of cD
概述基因组步移的应用方面介绍
①根据已知的基因或分子标记连续步移，获取人、动物和植物的重要调控基因，可以用于研究结构基因的表达调控。如分离克隆启动子并对其功能进行研究； ②步查获取新物种中基因的非保守区域，从而获得完整的基因序列； ③鉴定T-DNA或转座子的插入位点，鉴定基因枪转基因法等转基因技术所导致的外源基因的插入位
关于染色体步移的应用介绍
①根据已知的基因或分子标记连续步移，获取人、动物和植物的重要调控基因，可以用于研究结构基因的表达调控。如分离克隆启动子并对其功能进行研究；②步查获取新物种中基因的非保守区域，从而获得完整的基因序列；③鉴定T-DNA或转座子的插入位点，鉴定基因枪转基因法等转基因技术所导致的外源基因的插入位点等；④用于
基因组步移的应用介绍
①根据已知的基因或分子标记连续步移，获取人、动物和植物的重要调控基因，可以用于研究结构基因的表达调控。如分离克隆启动子并对其功能进行研究；②步查获取新物种中基因的非保守区域，从而获得完整的基因序列；③鉴定T-DNA或转座子的插入位点，鉴定基因枪转基因法等转基因技术所导致的外源基因的插入位点等；④用于
DNA转化实验指导2
1B. Cloning 1. A caveat on dephosphorylation: the most common reason for failure to obtain colonies is a result of adding too much BAP or CIP to
4－Reasons－Why－Your－Tank－Scale－Could－Be－Wrong
Do you rely on tank scales when filling, batching or tracking inventory? If so, accuracy limitations can diminish product quality, leading to mate
FISH－protocols－for－Drosophila2
3. Methods 3.1 RNA Probe Preparation 1. Different strategies can be used to prepare template DNA for synthesizing antisense RNA probes by in v
ELECTROPHORESIS－OF－DNA－IN－POLYACRYLAMIDE－GELS
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall: 165 x 130 mmMedium: 165 x 200 mmLarge: 165 x 260 mm5% Anal
A－protocol－for－cleaning－and－reusing－the－large－25－x－25－cm－plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays
PAC集成电路的封装特点
PAC(pad array carrier)凸点陈列载体，BGA 的别称(见BGA)。