4ReasonsWhyYourTankScaleCouldBeWrong
Do you rely on tank scales when filling, batching or tracking inventory? If so, accuracy limitations can diminish product quality, leading to material losses or even recalls. Learn how this new tank-design tool promotes more accurate tank weighing.Register for our tank-design webinarDownload our free guideline: 12 Key Considerations for Tank DesignLearn how to select the correct weigh module with our Weigh-Mo......阅读全文
4－Reasons－Why－Your－Tank－Scale－Could－Be－Wrong
Do you rely on tank scales when filling, batching or tracking inventory? If so, accuracy limitations can diminish product quality, leading to mate
蛋白质翻译后修饰的验证问题
Why are proteins, detected by mass spectrometry, not validated by site-specific antibodies?The modified motif could be detected by mass spectrometry (
Measurement－with－the－Light－Microscope
Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule ca
Green－lab－protocol－for－vacuum－infiltration－transformation－of－Arabidopsis
This protocol is adapted from protocols by Nicole Bechtold (Bechtold et al. 1993), Andrew Bent (Bent et al. 1994) and Takashi Araki. No claims are
Design－Weighing－Processes－with－Quality－in－Mind
June 15, 2016 - Greifensee, SwitzerlandWith QbD-Quality by Design-weighing processes help ensure excellent consistency in final productsBy Tobias Woel
Sea－Urchin－Animal－Maintenance
The following is written to help non-marine biologists use sea urchins occasionally in teaching; disregard if you have running sea water tanks in your
annealing－temp－for－degenerated－primer－－PCR－problem
PCR primers should be free of significant complementarily at their 3'-termini as this favours formation of primer-dimer which reduce product yield
Large－Scale－Tubulin－Preparation
Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosph
Largescale－Immunocytology
This protocol describes our method for preparing cells for immunofluorescence, in which all incubations and washes are performed in microtiter dishes.
Axolotl－Protocol:－Transplantation－of－the－Gill－Organ－Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Can－Pipetting－Damage－Your－Health
The Art of Ergonomics: Making the Work Fit the WorkerIn a working environment, use of ergonomic principles reduces levels of physical and mental s
CREATION－AND－USE－OF－YOUR－INFECTIOUS－VECTOR
实验概要CREATION AND USE OF YOUR INFECTIOUS VECTOR实验步骤Day 1 1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal
5G/NR－－OTA－（一）
OTA (Over The Air) OTA stands for Over The Air. In order to perform test a device with any test equipment, you need a way of connecting the device
Large－Scale－Tubulin－Preparation——2
III. Pouring a 1L Phosphocellulose (PC) ColumnResin: Whatman P11 Cellulose Phosphate -- fibrous cation exchanger(1 gram of PC swells to about 4 ml pac
Good－Pipetting－Practice
Good Pipetting PracticePipetting Techniques to Boost Your PerformanceImprove your data quality with Good Pipetting Practice™ (GPP™) – METTLER TOLE
How－do－you－synthesize－your－dsRNA
We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Desig
用肝癌小鼠模型研究人类HCC，why？
近日，来自美国贝勒医学院的研究人员在国际期刊nature communication发表一项最新研究成果，他们发现核外源性受体CAR与β catenin共同激活能够诱导小鼠已分化肝脏细胞的增殖，导致细胞增殖失控，发生肝癌。这项研究成果证明了肝癌小鼠模型与人类HCC具有直接关系，因此利用肝癌小鼠模
Large－Scale－Plasmid－Preps:－PEG－method
1. Grow 250 500 mL of bacteria overnight in LB with 50 µg/mL of ampicillin.2. Transfer culture to Nalgene bottles. Spin in SGA rotor at 6000 rpm, at
Dutch－firm－ASML－perfecting－microchip－shrink－for－tech－giants
A lens used in the manufacturing of semiconductors at Dutch company ASML, whose latest systems project blueprints of circuitry onto silicon wafe
Differential－Interference－Contrast
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)PrincipleDifferential interference microscopy requires several optical
PCR－Primers－For－Gene－Expression－Detection－or－Quantification
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
Small－scale－DNA－preps－for－Neurospora－crassa.
Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an ave
A－Method－for－MicroScale－Isolation－and－Purification－of－Gangliosides
A Method for Micro-Scale Isolation and Purification of GangliosidesStephan Ladisch~Director, Center for Cancer and Transplant Biology, Children's
Small－Scale－Yeast－Whole－Cell－Extract－for－IP
Grow 100 ml yeast cells in desired media overnight to an A600 of ~1.0 (0.6 to 1.2 works well). For growth in minimal media, 1 ml of a saturated overni
Large－Scale－Plasmid－Preps:－Qiagen/Cesium－Method
Most plasmids can be adequately prepped by kits containing DNA binding columns. These columns do not do a great job of separating plasmid DNA from con
线粒体的结构与功能
In an attempt to be concise and understandable, introductory level courses and textbooks frequently present concepts that are technically correc
Mitochondria－Structure－and－Function－－Theoretical－Details
Mitochondria Structure and Function - Theoretical DetailsIn an attempt to be concise and understandable, introductory level courses and textbooks freq
FDA爆出美国最脏药厂－产品每毫升5万多个菌落
近日，FDA官网爆出美国Sage制药有限公司产品经检查每毫升含菌量达57000CFU，并在其中检出致病菌——洋葱伯克氏菌。美国FDA批评他们对微生物检验方法和方法验证的不足和取样和设施的缺陷，并在在线清洁（CIP）系统内发现生成了生物膜具体缺陷如下： 1. Your firm failed t
Appendix－G:－Spectrophotometry
Figure G.1 Electromagnetic Radiation SpectrumFigure G.2. Schematic light transmissionFigure G.3. Use of the Spec 20A spectrophotometer or colorimeter
sothing－about－Genome－walking
Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it