LargeScalePlasmid,Cosmid,BAC,PAC,andFosmidDNAIsolation
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Implementation (by Feng Chen, Hau-Qin Pan and Fu Ying) Adapted from the Genome Sequencing Center, Washington Univ. ProtocolA smear of colonies of cosmid/BAC/PAC were picked and transferred into a 12X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bac......阅读全文
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CONTENT Transformation-Competent E. coli preparation Inoue "ultra-competent" methodRubidium chloride methodCosmid packaging protocol DNA Ligation an
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FIXATION－and－DNA－Staining－for－Cell－Cycle－Analysis
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