ProtocolsforLCMpreparationandanalysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Preparation and LCM of Paraffin Embedded Tissue SectionsFor both I & II Frozen & Paraffin-EmbeddedTissue SectionsD. Laser TransferE. DNA Extraction Protocol of LCM tissue and PCR for DNA analysisF. RNA Extraction Protocol of LCM ......阅读全文
REVERSE－TRANSCRIPTION－PCR:
REVERSE TRANSCRIPTION PCR:RNA -> LOTS OF DNAContentsReverse Transcription ReactionPolymerase Chain ReactionReverse Transcription Reaction:This provide
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（二）
Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total transcript in
多重PCR反应中关键因素和实验步骤（4）
琼脂糖凝胶与聚丙烯酰胺凝胶琼脂糖 . 长度彼此相差 30–40bp 的多重 PCR 产物在通常使用的 SeaKem 或 NuSieve(FMC BioProducts) 3% 的琼脂糖凝胶可以很好的区分开。在低电场强度下过夜电泳可以优化每个 PCR 产物条带的电泳效果，特别是当 PCR 产物小于 4
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
Immunohistochemistry－Protocol－for－Frozen－Sections
实验概要The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary
DNA标记
DNA标记（主要内容如下） DNA Labeling by Nick Translation Random Primed Labeling End-Labeling Purification of Labeled DNA Non-isotopic Labeling OthersDNA L
自动化的微流控芯片系统在单细胞中检测MicroRNA的异质性3
结论· 我们在C1TM单细胞自动制备系统开发了一种简洁的实验方案，能以最少的手工操作，在不到24小时内，平行处理高达96个单细胞，对其miRNA表达谱进行分析。· C1 miRNA STA实验方案使用了Life Technologies为miRNA优化过的试剂。特别的，Megaplex™ RT及
关于淋巴细胞脉络丛脑膜炎的简介
淋巴细胞脉络丛脑膜炎（LCM）系LCM病毒所致的急性传染病。本病的临床经过可有流行性感冒样症状至脑膜炎、脑炎等程度不等的表现。病程具自限性，预后良好。本病实为动物性的传染病，LCM病毒的天然宿主为褐家鼠。
淋巴细胞脉络丛脑膜炎的概述
淋巴细胞脉络丛脑膜炎（LCM）系LCM病毒所致的急性传染病。本病的临床经过可有流行性感冒样症状至脑膜炎、脑炎等程度不等的表现。病程具自限性，预后良好。本病实为动物性的传染病，LCM病毒的天然宿主为褐家鼠。
淋巴细胞脉络丛脑膜炎的定义及临床表现
淋巴细胞脉络丛脑膜炎（LCM）系LCM病毒所致的急性传染病。本病的临床经过可有流行性感冒样症状至脑膜炎、脑炎等程度不等的表现。病程具自限性，预后良好。本病实为动物性的传染病，LCM病毒的天然宿主为褐家鼠。
蛋白质翻译后修饰的验证问题
Why are proteins, detected by mass spectrometry, not validated by site-specific antibodies?The modified motif could be detected by mass spectrometry (
Phenomenex：提高LC/MS分析的速度和灵敏度的色谱柱
Phenomenex在ASMS2008上介绍了用于提高实验室通量的HPLC选项。海报的演讲将介绍Phenomenex的研究，关于分析生物基质中的药物化合物，和在反相LC－ESI+-MS/MS实验中，pH对于生物样品的影响。 Booth #131，用于LC/MS分析的理想色谱柱 Jupiter
The－Impact－of－Harmo...－（一）
实验概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and reliable a
Enrichment－of－PBMCs－with－monocytes－(The－Science－Advisory－Board)
DescriptionThis protocol is used in our lab to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protoc
Milestone微波化学平台新品发布会邀请函
尊敬的女士/先生：您好！ 意大利Milestone公司2014微波化学平台新产品发布会将于9月25日召开，Milestone公司总经理Diego先生和中国区总代北京莱伯泰科公司，诚意邀请您莅临参加，共同体验分享Milestone新品带给您全新的惊喜！ Milestone公司生产微波
分光光度计知识
With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.
Quantification－made－easy
With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.
碳水化合物分析
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a given s
分光光度计的使用
With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories.
MATRIXMF傅立叶变换红外光谱仪英文参数
Robust DesignThe MATRIX series' award winning design protects the optics in a dedicated sealed compartment. The permanently aligned RockSolid™
淋巴细胞脉络丛脑膜炎的病因是什么？
淋巴细胞脉络丛脑膜炎（LCM）系LCM病毒所致的急性传染病。本病的临床经过可有流行性感冒样症状至脑膜炎、脑炎等程度不等的表现。病程具自限性，预后良好。本病实为动物性的传染病，LCM病毒的天然宿主为褐家鼠。 本病的发病机理尚未完全阐明，病毒首先侵入呼吸道时，可在上皮细胞内大量繁殖，故不少患者表现
MetalEnhanced－Immu...－（二）
实验步骤1. Preparation of Fluorescein-Doped PVA FilmsThe 0.5% PVA solution containing disodiumfluorescein was spin-coated on slide substrates at 3,000 rp
Small－scale－DNA－preps－for－Neurospora－crassa.
Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an ave
Immunofluorescence－Labeling－of－Cells
实验概要Antibodies are an important tool for demonstrating both the presence and the subcellular localization of an antigen. Cell staining is a very ver
Construction－of－BAC－Libraries：Megabase－DNA－Isolation
Megabase DNA IsolationMegabase-size DNA isolation from plantsTo construct large insert DNA libraries in BAC and YAC vectors, methods must be developed
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
DNA的酶学操作
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge
自然染色质免疫沉淀实验设计
1. The preparation of native chromatin from cultured human cells1.1.Cultured cells (e.g. HL-60 or lymphoblastoids) are grown to a density of approxima
ES－Cell－Culture－and－Manipulation2
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
Basics－of－Electrochemical－Impedance－Spectroscopy（九）
Multiple ModelsThe impedance spectrum in Figure 25 shows two clearly defined time constants.Figure 25. Two Time Constant SpectrumThis spectrum can be