PreparationandStainingofParaffinSections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and approved euthanasia techniques. Tissues to be fixed and processed should be cut to a size no larger than 3mm thick. Let tissues fix in 10% formalin at room temperature for 8 hours but not to exceed 24 hours. For small rodent tissue, it is recommended to fix tissues for 4-......阅读全文
Preparation－of－Mouse－Neutrophils
实验概要Preparation of Mouse Neutrophils实验步骤Mice：8-16 weeks old malePrewarm buffer to room temperatureBuffer A : Ca2 /Mg2 ‐free Hank’s buffered saline so
Competent－Cell－Preparation
实验概要Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate f
PREPARATION－OF－MICROINJECTION－PIPETTES
INJECTION AND HOLDING PIPETTESThe glass capillary tubing used should be thin walled, borosilicate glass without a fibre.e.g. Clark Electromedical Inst
Preparation－of－Agar－plates
Prepare media and add 1.5 agar before autoclaving it (15g per liter). After autoclavation, cool the media in a 55 degree waterbath. Do not allow the s
Metaphase－chromosome－preparation
Materials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents, Best.-Nr. 295892) cell cuture flask
Preparation－of－Phage－Lysates
Preparation of Phage LysatesInoculate 5 ml of lambda-broth in a glass culture tube with a single colony of an appropriate host strain of E. coli. Incu
DGK－Membrane－Preparation
Reagents:Bacterial strainE. coli N4830/pJW10LB amp media50 µg/ml ampicillinHigh salt bufferfor 1 L50 mM KH2PO4 6.8 g150 mM KCl 11.18 g50 mM sodium pyr
Preparation－of－Mouse－Neutrophils
实验步骤Mice：8-16 weeks old malePrewarm buffer to room temperatureBuffer A : Ca2 /Mg2 ‐free Hank’s buffered saline solution [HBSS; Invitrogen, Grand Isla
PREPARATION－OF－SEQUENCING－GELS
MATERIALS:2-glass plates1 sharks -tooth comb and spacersWhatman 3 mm paper30 or 40% acrylamide-bis (19:1)10X TBEurea10% ammonium persulfateTEMED60 cc.
Immunofluorescence－...
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
转基因
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Core)Thi
免疫资料——免疫学常用简称介绍
1：抗体查询常用名词 Reactivity （物种与抗体反应）：Species with which the antibody reacts Host Species （宿主）：Host in which the antibody was generated Applicati
Antigen－Retrieval
实验概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortunat
Immunofluorescent－Staining－of－Mouse－and－Rat－Leukocytes
I. ProcedureHarvest cells from tissue, preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cell
Cell－Surface－Immunofluorescence－Staining－Protocol
实验概要A method of identifying and enumerating specific cell types in a heterogeneous population of cells by enhancing the specific staining of desired
Immunofluorescent－staining－of－Sea－Urchin－embryos
1. Transfer fixed embryos to microfuge tubes. Allow to settle for 10 minutes.Gently remove most of the liquid.2. Add 100 ul antibody to one tube and 1
SSR－GEL－and－Silver－Staining－Protocol
I. EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking platformsH
Methylene－Blue－DNA－staining－protocol
Methylene Blue DNA staining protocolProtocol:Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained ge
Intracellular－Immunofluorescent－Staining－for－Flow－Cytometry
IntroductionA modification of the basic immunofluorescent staining and flow cytometric analysis protocol can be used for the simultaneous analysis of
组织学——试剂和溶液
Fixative Preparation10% Formal Saline Preparation (Nottingham Pathology Lab) 4% Paraformaldehyde Preparation (Nottingham Pathology Lab) Fixatives (XM
immunofluorescence－of－rabbit－antimurine－leptin－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine leptin by Peprotech.实验步骤The following protocol used Trichur
原位PCR
About in situ PCR (Applied Biosystems)Basic information about in situ PCR and its applications.The In Situ PCR: Amplification and Detection in a Cellu
immunofluorescence－of－rabbit－antimurine－RELMβ－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMβ by Peprotech.实验步骤The following protocol used B6 mice
Immunohistochemistr...
实验概要Peprotech provides a immunohistochemistry protocol for Mouse Anti-Human RANTES.实验步骤The following protocol used formalin-fixed paraffin-embedded hu
immunohistochemistry－of－mouse－antihuman－Eotaxin2－by－Peprotech
实验概要The following protocol provides a method of immunohistochemistry of mouse anti-human Eotaxin-2 by Peprotech.实验步骤The following protocol used formal
流式细胞仪技术专辑
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excitation sour
Sample－preparation－(analytical－gels)
Sample preparation and solubilization are crucial factors for the overall performance of the 2-D PAGE technique. Protein complexes and aggregates shou
Easy－YAC－Preparation－Method
YAC TRANSFORMATION OF C. ELEGANS USING TOTAL YEAST GENOMIC DNA[This method is described in The Worm Breeder's Gazette (1997) A. Davies and J. Shaw
Preparation－of－Sonicated－Human－DNA
Purpose:To break up high molecular weight human placental DNA into fragment sizes of 500 bp or less which can be used as competitor DNA in Southern an
Preparation－of－tubulin2
DAY 2: Cycling preparation of MT protein.Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport