PreparationandStainingofParaffinSections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and approved euthanasia techniques. Tissues to be fixed and processed should be cut to a size no larger than 3mm thick. Let tissues fix in 10% formalin at room temperature for 8 hours but not to exceed 24 hours. For small rodent tissue, it is recommended to fix tissues for 4-......阅读全文
Liposome－Preparation
Liposome PreparationOBJECTIVE:Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation m
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
CAM－preparation
8 eggs per day, day 7- day 13 cut CAM, wash in precooled PBS,in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on icecut in pieces in petri dish on icecentri
Preparation－of－tubulin
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have d
Apoptosis－TUNEL－Assay－(frozen－sections)
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
Extraction－of－RNA－from－Frozen－Sections
RNA Extraction from Frozen Tissue Sections Tissue Handling: Note that all unfixed human tissue should be handled as BioSafety Level 2 materials (wear
细胞遗传学——染色体
Chromosome Staining and Banding Technique (Primate Cytogenetics Network)Protocols for different staining method, each is in great detail. Karyotype A
显微镜技术——电子显微技术
The Transmission Electron Microscope (TEM) (HEI)An explanation of how the TEM works. TEM Specimen Preparation (HEI) Serial Sectioning (Walter Steffe
免疫酶细胞化学实验技术4
三、ICC在细胞功能研究中的应用 形态学研究目的之一是揭示组织细胞结构特征及其功能意义。利用ICC显示给与细胞增殖标记物，是组织细胞学研究中形态和功能相结合的重要手段之一。目前常用的细胞增殖标记物有4种，Brdu (5—bromo—2--deoxyuridine), DNA polymerase
Immunohistochemistr...
实验概要Peprotech provides a immunohistochemistry protocol for Mouse Anti-Human MCP-1*.实验步骤The following protocol used formalin-fixed paraffin-embeddedhum
蛋白质电泳
蛋白质电泳（主要内容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·
细菌检测
Gram Staining (+\-) (William H. Heidcamp) Gram-Staining Procedure (MEDIC, U of Texas)Very nice and detailed method description for Gram staining Aci
Actin－StainingActin－Staining－Protocol
实验概要Invitrogen offers several fluorescent and biotinylated phalloidin and phallacidin derivatives for labeling F-actin. These phallotoxins, isolated
Gramstaining－Procedure
Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should
Intracellular－Cytokine－Staining－Protocol
实验概要A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surf
Direct/Indirect－Staining－Protocol
Use this Protocol for Directly or Indirectly staining cells for Flow Cytometric Analysis1) Dilute cells to 5x10^6 cells/mL2) Aliquot 100uL of cells pe
High－resolution－negative－staining
High resolution negative staining(From Valentine et al, 1968. Biochemistry 7:2143-52)Rationale: For the highest resolution with negative staining, the
Staining－Methods－for－cell－death
The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead
免疫资料——免疫学常用简称介绍
1：抗体查询常用名词Reactivity （物种与抗体反应）：Species with which the antibody reactsHost Species （宿主）：Host in which the antibody was generatedApplications（应用） ：Speci
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for l
Antigen－Retrieval
实验概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortun
DNA－Extraction－from－Frozen－Tissue－Sections
Tissue collection, storage, microdissection, sectioning: See separate protocol.Tissue handling: Note that all fresh tissue should be handled as BioSaf
Detection－of－apoptotic－process－in－situ－using－immunocytochemical2
B) TUNEL in situ procedureB.2.1 Materialsproteinase K (pK) (A2), H2O2 , TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1), seru
Rat－Liver－Preparation
实验概要The procedure presented below describes a method for preparing rat liver.主要试剂1. Aluminum Foil2. Liquid Nitrogen3. Dry Ice4. Ph
CELL－MEMBRANE－PREPARATION
I. Solutions: A. Ca and Mg free Phosphate Buffered Saline (PBS) solution, buffered with 0.02M Hepes. pH=7.4 B. Ca and Mg free PBS, buffered with
Preparation－of－human－platelets
Preparation of human platelets 1. Human blood was taken from drug-free volunteers on the day of the experiment using acidic citrate dextrose
HELPER－PHAGE－PREPARATION
HELPER PHAGE PREPARATION1. Grow an overnight of NM522 in NZCYM medium.2. Dilute overnight 1:100 and grow to an A600 = 0.3 (@2.5 x 108 cells/ml).3. Inf
Preparation－of－Polyacrylamide－Gels
1. Prepare 20X TBE as:216 g Tris Base110 g Boric Acid80 mL 500 mM EDTA, pH 8.0700 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Prepare Acrylamide so
Lambda－DNA－Preparation
Lambda DNA PreparationThis is a plate method that gives very good yield for cloning. I have combined the Promega and Maniatis protocols.Solutions T-TY
Plasma－and－Serum－Preparation
实验概要Serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot. The clot is removed by centrifugation and the