MeasurementwiththeLightMicroscope
Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule can be used to measure any planar dimension in a microscope field since the ocular can be turned in any direction and the object of interest can be repositioned with the stage manipulators. To measure the length of an object note the number of ocular divisions spanned by ......阅读全文
临床常用的细菌检验技术
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Immunofluorescence－...
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
胚胎和成年斑马鱼眼情的组织学准备
INTRODUCTIONThis protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapt
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重新打开生物学大门：light－sheet晶格层光显微镜
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In－Vivo－Imaging－of－Far1
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
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Immunofluorescence－Labeling－of－Cells
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Science发表：癌细胞移动－神经发育－新技术都能“看得到”
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Protocol－to－Count－Cell－Number－of－Preimplantation－Embryos
Protocol to Count Cell Number of Preimplantation Embryos using Nuclear Staining with Hoechst 33342 or DAPI Introduction The following is a simple pro
How－to－perform－automated－counts－of－fluorescently－stained－cells.
NoteThis protocol describes semi-automated cell counts using fluorescently labeled cells, a hemocytometer and ImageJ software. The hemocytometer is n
Spectrophotometry
A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the sol
Histochemistry－－Introduction
A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, bra
Live－imaging－with－Drosophila－tissue－culture－cells2
Materials & ReagentsDrosophila Schneider S2 cellsSchneiders Medium (GIBCO/Invitrogen), 10% fetal calf serum, Antibiotics (Sigma A5955)Depression slide
Light－|－AI+椭偏仪：从“众里寻它”到“灯火阑珊”
薄膜材料在微电子、光电子、生物、医药等领域高端器件的制造中不可或缺。为了提高器件的性能指标，人们需要精确地测量与掌握薄膜的特性参数，比如光学常数(n, κ)和薄膜厚度(d)就是两种重要的材料参数。前者同材料组分、带隙、结晶度等信息密切相关，而薄膜厚度的微小误差会对器件性能的造成显著影响。椭偏仪因
Silver－Enhancement－...
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检测机构使用流动成像显微镜来表征蛋白质聚集体（一）
Contract Lab Using Flow Imaging Microscopy to Characterize Protein Aggregates 检测机构使用流动成像显微镜来表征蛋白质聚集体Posted by Joyce BrownJoyce Brown发表Dan Berdovich kn
Immunohistochemistr...
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In－Situ－Hybridization－to－Somatic－Chromosomes－in－Drosophila
In Situ Hybridization to Somatic Chromosomes in DrosophilaAbby F. DernburgINTRODUCTIONIn situ hybridization was originally developed as a technique fo
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Differential－Interference－Contrast
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5G/NR－－OTA－（二）
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LaVision双光子显微镜多线扫描双光子成像（三）
2.2.多线TPLSM中通过成像检测释放光 在单光束TPLSM中，光电倍增管PMT或者雪崩二极管APD可以很方便地用于释放光检测，由于双光子激发的原理，激发只发生在激光焦点处。因此，用于屏蔽离焦光线的共焦小孔变得不必要，并且可以使用NDD检测。这意味着激发光不会被送回扫描镜，而是直接进入位于靠
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Appendix－G:－Spectrophotometry
Figure G.1 Electromagnetic Radiation SpectrumFigure G.2. Schematic light transmissionFigure G.3. Use of the Spec 20A spectrophotometer or colorimeter
Isolation－of－kidney－glomeruli－from－mice
Isolation of mice glomeruli1. Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcohol; 17
Immunofluorecence－P...
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Autoradiography
MaterialsH-Thymidine, specific activity of 2.0curie/millimoleOnion sets, jars and toothpicksAlcohol-acetic acid fixativeMaterials for feulgen reaction