MeasurementwiththeLightMicroscope

Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule can be used to measure any planar dimension in a microscope field since the ocular can be turned in any direction and the object of interest can be repositioned with the stage manipulators. To measure the length of an object note the number of ocular divisions spanned by ......阅读全文

Immunofluorescent-Localization-of-Tubulin

LEVEL IIMaterialsCoverslip cultures of an appropriate monolayer cell linePhosphate buffered saline (PBS)Acetone/Methanol (absolute) in a 50:50 volume

Invitro-Phagocytosis-Assay-of-Macrophages

IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular pr

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摘要:与传统正投影显微图像分析方法不同,本文应用斜投影法,通过变换观测颗粒群的角度,依据获得的光学显微镜下颗粒群的信息,推导出了颗粒群厚度的计算公式,并讨论了厚度分辨能力与倾斜角、放大倍数之间的关系,同时应用实例证明了该方法的可行性。 关键词:斜投影;显微图像分析;三维重构;体视学中图分类号:TH7

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ResultsBefore demonstrating the fast terahertz detection, we first characterize the electrical and optical performances of the terahertz QWP. The

使用量子级联激光器的可调谐长波红外陷波滤波器的...1

使用量子级联激光器的可调谐长波红外陷波滤波器的性能特征我们描述了在可调谐整个光谱范围内使用量子级联激光器(QCL)在8至12微米的长波红外中操作的可光谱调谐微工程陷波滤波器的设计和性能表征。滤波器的设计基于导模共振现象。器件结构由平面波导顶部的亚波长介电光栅组成,该光栅使用高折射率透明介电材料,即折

Protocol-for-competitive-RTPCR

For quantifying mRNA, we use a competitive RT-PCR protocol with internal standard RNAs. These are added in a defined quantity to the RNA sample prior

3HThymidine-Uptake-by-Cultured-Cells

Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)

相位式原子力显微镜

相位式原子力显微镜(Phase Ima ging Force Microscope)原子力显微镜在轻敲式AFM(tapping mode)操作下,量测及回馈因表面抵挡及黏滞力的作用,会引起振动探针的相位改变量,而抵挡及黏滞力的差异为不同材料性质引起,因此有机会用相位差(Phase la g)来观察表

Nflight检测试剂盒助力神经病学领域研究新发现(二)

图2. 健康对照组(a)和糖尿病患者组(b)的血液Nf-L水平与eGFR(估计的肾小球滤过率)呈正相关 3. Plasma neurofilament light protein correlates with diffusion tensor imaging metrics in frontote

Nflight检测试剂盒助力神经病学领域研究新发现(三)

关于Simoa Nf-L检测试剂盒研究表明,神经丝轻链蛋白Nf-Light (Nf-L)是与脑损伤、脑中风、多发性硬化症、痴呆和其他神经退行性疾病相关神经元损伤的生物标志物,是研究神经性疾病最有力的工具之一。Simoa Nf-L 检测试剂盒不仅可以检测脑脊液(CSF)中的Nf-L水平,还能定

Nflight检测试剂盒助力神经病学领域研究新发现(一)

关于Simoa Nf-L检测试剂盒研究表明,神经丝轻链蛋白Nf-Light (Nf-L)是与脑损伤、脑中风、多发性硬化症、痴呆和其他神经退行性疾病相关神经元损伤的生物标志物,是研究神经性疾病最有力的工具之一。Simoa Nf-L 检测试剂盒不仅可以检测脑脊液(CSF)中的Nf-L水平,还能定

Assay-of-superoxide-dismutase-activity3

Botanical Bulletin of Academia Sinica, Vol. 37, 1996The method using NBT as a superoxide radical competitor and a color indicator was also explored to

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  描述:   反光标线亮度,国际上用逆反射系数来表示,单位mcd/lx/m2。其物理意义是:在单位光照条件下,单位面积上产生的亮度值。将仪器置于被测标线的上面,该仪器以数字的形式显示出逆反射系数值,它反映了行车条件下司机对标线反光亮度的客观评价。该仪器由测量主机、电源、充电器、标准板组成。  

标线逆反射系数测量仪特点

  描述:   反光标线亮度,国际上用逆反射系数来表示,单位mcd/lx/m2。其物理意义是:在单位光照条件下,单位面积上产生的亮度值。将仪器置于被测标线的上面,该仪器以数字的形式显示出逆反射系数值,它反映了行车条件下司机对标线反光亮度的客观评价。该仪器由测量主机、电源、充电器、标准板组成。  

Exercise-12.10--Establishment-of-a-Primary-Culture

Exercise 12.10 - Establishment of a Primary CultureLEVEL IIIMaterialsChick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scis

HThymidine-Uptake-by-Cultured-Cells

H-Thymidine Uptake by Cultured CellsLEVEL IIMaterialsFibroblast cells in log phase growthCa, Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaral

Tissue-Culture-Methods1

I. TYPES OF CELLS GROWN IN CULTURETissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often us

XC-Assay-of-MoMLV-Virus-Stocks

MaterialsWildtype MoMLV virus aliquot.  Stored at -80ºC.Medium:  DMEM + 10% FBSNIH 3T3 TK- cellsXC cellsPolybrene 1000x stock = 4 mg/mL, sterile filte

MetalEnhanced-Immu...-(二)

实验步骤1. Preparation of Fluorescein-Doped PVA FilmsThe 0.5% PVA solution containing disodiumfluorescein was spin-coated  on slide substrates at 3,000 rp

Effect-of-UV-Treatment-on-Early-Development-of-Sea-Urchin-Embryos

Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh

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OPERATION OF B&L SPEC. 20The most commonly encountered spectrophotometer is one manufactured by Bausch and Lomb and known as the Spec 20. The 20 refer

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提要:利用光干涉的简化模型讨论了动态光散射中光子相关谱测量系统的空间相干性要求的物理本质。利用相干面积概念对光子相关谱测量系统空间相干性判据的几种常见表述进行了规范。提出了一种具有普遍意义的简明判据。 关键词:光子相关谱;动态光散射;空间相干性;相干面积;信噪比On the Spatial Coh

Matrigel-invasion-assays

OverviewMatirgel is considered as basement membrane and generated from EHS sarcoma. Matrigel contains not only basement membrane components (collagens

Immunocytochemistry...

实验概要The method provides a guideline procedure for staining of cell cultures using immunofluoresence.实验步骤1. General procedure    1) Coat coverslips wit

3HThymidine-Uptake-by-Cultured-Cells

3H-Thymidine Uptake by Cultured Cells  OverviewA radioactive method for measuring cultured cell growth with 3H-thymidine is described here. MaterialFi

上海光机所提出基于结构光投影的快速三维重建方法

  三维扫描仪(3D scanner) 是一种科学仪器,用来探测并分析现实世界中物体的三维形状。在工业设计、逆向工程、机器人导航、医疗检查、刑事鉴定、文物保护、电影制片、游戏创作等众多领域中都有应用。双目三维扫描系统中关键是如何解决对应问题,即如何确定左右两幅图像中的两个像点是物体表面同一

Hints-and-precautions-for-the-care,-feeding-and-breeding-of-Neurospora2

9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A si

Peripheral-blood-“endothelial-progenitor-cells”

EPC Isolation and Characterization1. EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf

将严谨的QbD(质量源于设计)引入分析方法开发之中-四

 让AQbD更加简便的工具 QbD、AQbD强调对过程的充分理解,而非简单着眼于适用特定样品种类的一系列条件。这种方法的潜在优势已经凸显,但是要获取更多的信息,需要进行更大规模的实验。因而能够缓解与本研究相关负担的工具,自然也就受到欢迎。 图6:Mastersizer 3000测量管理器呈现的分散趋