Highresolutionnegativestaining
High resolution negative staining(From Valentine et al, 1968. Biochemistry 7:2143-52)Rationale: For the highest resolution with negative staining, there should be little or no support film, but some support is necessary to hold the protein. In this method the proteins are supported by a carbon film and "embedded" in a film of uranyl acetate. The film is cast on mica, which provides the cleanest possibl......阅读全文
Hybridization－of－High－Density－Arrayed－BAC－Nylon－Filter－Blots
Protocol for hybridization of high density arrayed Bacterial Artificial Chromosome nylon filter blots with 100 PCR isolated Unigene cDNA inserts, pool
Proteome－and－Phosphoproteome－Quantification－by－DIA－on－Orbitrap－（一）
Overview1) Purpose: Data Independent Acquisition (DIA) has been applied for large-scale protein and PTM quantification on the Thermo Scientific ™
Histochemical－staining－of－sea－urchin－embryos－for－(AP)－enzyme－activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples, tube of AP substrate buffer and tu
Whole－mount－antibody－staining－ofzebrafish－embryos－formarkers－ofsegmentation
1. Dechorionate 26 hr embryos (pharyngula stage) carefully with two fine forceps.Transfer to fixative (1% formaldehyde in PBS). Fix for 1 hour rocking
SCI论文中怎样描述TEM结果？HRTEM结果描述样品晶格条纹等
实例一：High-resolution transmission electron microscopy (HRTEM) was used to characterize the catalysts after reaction (Figure 4). The Au-0.0 sample shows
使用CCCadvanced™FN1无异源耗材培养人多能干细胞（三）
Figure 2: hiPSCs proliferation during long-term expansionA. Cumulative cell population doublings for hiPSCs cultured on the FN1 motifs surface as we
Multiple－studies－with－a－single－experiment:－The－Power－of－...（二）
A Better Multiplexing Method– Isobaric Mass Tagging •Less MS1 Complexity •Increased Throughput Concurrent MS analysis of multiple samples •Less
JC1分析线粒体膜电位的方法2
3. COMMENTARY3.1 Background information The technique of JC-1 staining has been developed with the intent to detect DY in intact, viable cells. For th
LIVE/DEAD®－Fixable－Dead－Cell－Stain－Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
TEM－Specimen－Preparation：Preparative－Techniques－for－the－TEM
For routine transmission electron microscopy (TEM), it is generally accepted that specimens should be thin, dry and contain molecules which diffract e
可靠的CCCadvanced－FN1无异源耗材支持人间充质干细...（二）
Materials and MethodsShort-term cell growth evaluationLonza™ Poietics™ human mesenchymal stem cells (hMSC-BM, PT-2501, Lonza) derived from normal ad
发布新型Q－Exactive－直接竞争2.5亿美元的QTOF市场
Thermo Fisher 'Going Directly After' $250M Q-TOF Market with Release of New Q Exactive Mass SpecDENVER — With the release of its new Q Exactive mass s
Culture－of－human－renal－proximal－tubule－cells－and－renal－cortical－fibroblasts
1. The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibroblasts (CF) is described in detail.2. Renal cor
岛津生命科学推出Axima－QIT质谱仪
SHIMADZU BIOTECH LAUNCHES AXIMA-QIT™- THE LATEST IN MASS SPECTROMETRY Unique instrument aimed at proteomics research January 15, 2002 Shimadzu Biot
蛋白质电泳技术
Serum Protein Electrophoresis Tricine/Polyacrylamide Gel ElectrophoresisUsed for pilin processing analysis but generally useful for resolution of smal
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十一）
Fixture Testing. Fixture testing consisted of vacuum, heating, pressure, and microwave testing.Pressure Testing. Initial pressure testing of thin sa
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（一）
Dr. Stephen C. Bates，Thoughtventions Unlimited LLC40 Nutmeg Lane Glastonbury, CT 06033EXECUTIVE SUMMARYThe Problems.Windows that transmit high powe
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（五）
Single crystal sapphire is not a typical optical material, with a standard strength not much lower than the steel described above, since the ave
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（九）
3) 50 mm Disks. Four 50 mm diameter, 2 mm thick disks were specially polished by Meller Optics to try to obtain strengthening disks. A previous po
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（八）
Strength Prediction. Large deflection theory solves for the deflection of a disk as a function of loading and boundary condition. Stress is a deri
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十）
through a boundary conductance, h, on faces at z = +/- L.is controlled by the parameter hL/k, the Biot Number, where k is the thermal conductivity
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（二）
Summary of ResultsThe important objective of Phase II research was achieved: to fabricate and test a prototype high power sapphire microwave windo
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十二）
The input port is connected to the high power gyrotron source and the output port is connected to a dummy load to absorb any power not coupled i
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（七）
The failure data is shown for the 12.5 mm diameter disks is shown in Fig. 12. The average failure pressure for the standard disks is 12 MPa, whe
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（四）
Membrane effects describe the case where the disk stretches rather than bends. Figure 5 shows the differences in the deflection and stress for the
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（十三）
The diameter uptaper from 38.3 mm to 63.5 mm was then fabricated to match the diameter of the resonant ring and windows to be tested. A square r
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（三）
Sapphire Window Development and Testing.The effort of this work was devoted to fabricating ultrathin strengthened sapphire disks and to accurately
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（六）
windows were confused by the simultaneous deflection of the windows and the O-ring before the window came in contact with the flange. The recesses
Bruker－Daltonics在ASMS上推出革命性的超高分辨TOF质谱maXis
2008年6月丹佛，商业电讯，2008年6月2日，布鲁克道尔顿推出maXis™，革命性的新的电喷雾－超高分辨率串联飞行时间 (UHR-Qq-TOF)质谱仪，新型的maXis 展示了布鲁克在设计尖端质谱方面的领先地位，以满足未来的应用需求，包括：小分子鉴定，代谢组学，定量蛋白质组学，生物标志物的发现及
Cell－Staining－for－SenescenceAssociated－betaGalactosidase－－Activity
DescriptionCell Staining for Senescence-Associated â-Galactosidase (SA-â-Gal) Activity Procedure1. Carefully remove the growth media from the cell cul