Asemipermanentmountingmediumforimmunofluorescencemicroscopy
A semi-permanent mounting medium for immunofluorescence microscopyMaterials6gm glycerol2.4gm mowiol6ml distilled water12ml Tris buffer 0.2M at pH 8.5MethodWeigh out the glycerol in a 50ml plastic tube and add the mowiol. Stir thoroughly. Add water and leave for 2hr at room temperature. Add the Tris buffer and incubate at approximately 53¡C until the mowiol dissolves, stirring occasionally. Clarify by centrifugation a......阅读全文
Negative－Stain－Electron－Microscopy－of－Microtubules
Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of
CarbohydrateSpecific－Adhesion－of－Intact－Cells－to－Resolved－Glycolipids－on－T
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ImmunohistochemistyFluorescence－Protocol1
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'&
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EPON－resin－mixture－for－transmission－electron－microscopy
EPON resin mixture for transmission electron microscopyFor Epon WPE 153:~120 ml~60 ml~30 mlMix A:Embed 81244 ml22.1 ml11.1 mlDDSA67 ml33.3 ml16.7 mlMi
免疫电镜(Immune－electron－microscopy)原理
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Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
ImmunohistochemistyFluorescence－Protocol2
Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
实验概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)
Use－of－SemiThin－Cryosections－for－Light－Microscopy.
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DAPI－Counterstaining－Protocols
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Immunohistochemistry－Protocol－for－ParaffinEmbedded－Sections
实验概要The following is a general procedure guide for preparation and staining of formalin-fixed, paraffin-embedded tissues using a purified, unconjuga
Immunohistochemistr...
实验概要Peprotech provides a immunohistochemistry protocol for Mouse Anti-Human RANTES.实验步骤The following protocol used formalin-fixed paraffin-embedded hu
immunohistochemistry－of－mouse－antihuman－Eotaxin2－by－Peprotech
实验概要The following protocol provides a method of immunohistochemistry of mouse anti-human Eotaxin-2 by Peprotech.实验步骤The following protocol used formal
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
INTRODUCTIONThe Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem
Immunohistochemistr...
实验概要Peprotech provides a immunohistochemistry protocol for Mouse Anti-Human MCP-1*.实验步骤The following protocol used formalin-fixed paraffin-embeddedhum
Immunocytochemistry...
实验概要The method provides a guideline procedure for staining of cell cultures using immunofluoresence.实验步骤1. General procedure 1) Coat coverslips wit
DAPI－Nucleic－Acid－Stain
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Noble－Agar－Assay
DescriptionCancer cells do not show anchorage and contact inhibition of growth. To assess the anchorage and contact independent growth of cells, noble
哺乳动物RNAi技术－Mammalian－RNA－Interference
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原子力显微镜的工作原理就是将探针装在一弹性微悬臂的一端，微悬臂的另一端固定，当探针在样品表面扫描时，探针与样品表面原子间的排斥力会使得微悬臂轻微变形，这样，微悬臂的轻微变形就可以作为探针和样品间排斥力的直接量度。一束激光经微悬臂的背面反射到光电检测器，可以精确测量微悬臂的微小变形，这样就实现了通过检
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扫描电镜分析可以提供从数纳米到毫米范围内的形貌像，观察视野大，其分辩率一般为6纳米，对于场发射扫描电子显微镜，其空间分辩率可以达到0.5纳米量级。其提供的信息主要有材料的几何形貌，粉体的分散状态，纳米颗粒大小及分布以及特定形貌区域的元素组成和物相结构。扫描电镜对样品的要求比较低，无论是粉体样品还是大
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic1
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Basic－Fluorescent－in－situ－Hybridization－(FISH)
实验概要Fluorescence in situ hybridization method is a kind of physical map drawing method, use fluorescent element mark probe, to detect probe and spli
The－UnderAgarose－Migration－Assay2
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the best magni
E.－Immunohistochemi...
实验概要We provide a guideline procedure and tips for staining of paraffin embedded sections, including antigen retrieval, chromogenic detection and flu
Acetobacter－Medium－(醋酸菌培养基)的成分和适用范围
Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10gCaCO3 20g Agar (琼脂) 15gDistilled water (蒸馏水) 1000ml Adjust (调) pH to 6.8适用范围：恶臭醋酸杆菌混浊变种
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经历了天然培养基、合成培养基后，无血清培养基和无血清培养成为当今细胞培养领域的一大趋势。采用无血清培养可降低生产成本，简化分离纯化步骤，避免病毒污染造成的危害。 无血清培养基(serum free medium,SFM):是不需要添加血清就可以维持细胞在体外较长时间生长繁殖的合成培养基。但是