DarkFieldViewing
Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scattered by objects on the slide can reach the eye (figure 2). Instead of coming up through the specimen, the light is reflected by particles on the slide. Everything is visible regardless of color, usually bright white against a dark background. Pigmented objects are often ......阅读全文
太赫兹信号源Terahertz－sources（一）
1. Introduction1.1. Terahertz bibliometricsBefore turning to the topic of terahertz sources, a brief survey of the growth of the terahertz field a
欧盟就三氯硝基甲烷的农药风险评估发布同行评审
据欧盟食品安全局（EFSA）消息，2020年3月5日，欧盟食品安全局就活性物质三氯硝基甲烷（Chloropicrin）的农药风险评估发布同行评审。 经过评估，在将三氯硝基甲烷作为草莓、番茄、辣椒和木本作物（梨果、核果、柑橘、橄榄）土壤熏蒸剂的基础上，欧盟食品安全局提出了适用于监管风险评估的可靠
太赫兹信号源Terahertz－sources（三）
4. Terahertz surface emission—transient currentsTerahertz emission can result from a changing dipole. The terms current surge, surge current or transi
Keynote－Speaker：Mike－McMullen
Mike McMullen Mike McMullen was appointed Agilent CEO in March 2015, replacing William (Bill) Sullivan. McMullen has more than 30 years of industry
合成孔径雷达原理（一）
Theory of Synthetic Aperture Radar合成孔径雷达原理Electromagnetic TheoryUnlike optical and infrared imaging sensors which are inherently passive, meaning
Cell－Counting/－LiveDead－Discrimination
This is a microscopy based application for definitive discrimination of live and dead cells. Trypan Blue exclusion notoriously over estimates the numb
MTT－Cell－Proliferation－Assay
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondri
关于米格列醇的物化性质介绍
一、米格列醇的物化性质： 外观与性状：白色至淡黄色晶体粉末 密度：1.458 g/cm3 熔点：114ºC 沸点：453.7ºC at 760 mmHg 闪点：284.3ºC 折射率：1.597 储存条件：Store in original container in a cool
基金委公布09年外国青年研究基金获资助项目
共40个项目获得资助 据国家自然科学基金委员会网站消息：2009国家自然科学基金外国青年研究基金受理和评审工作已经全部完成，共40个项目获得资助，现将获资助项目批准清单公布如下： 序号 科学部编号 申请人姓名 国籍 国内依托单位 课 题 名 称 1 10
Use－of－the－B.D.－FACS－or－Calibur－Flow－Cytometers
Reservations:Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pa
细胞周期的流式细胞伩检测实验方法（PI，Brdu)1
ANALYSIS OF CELL CYCLE Miriam Capri and Daniela Barbieri Dept. Biomedical Sciences, Sect. General Pathology,Via Campi, 287, University of Modena, 4110
场发射式电子枪
场发射式电子枪则比钨灯丝和六硼化镧灯丝的亮度又分别高出 10 - 100 倍，同时电子能量散布仅为 0.2 - 0.3 eV，所以目前市售的高分辨率扫描式都采用场发射式电子枪，其分辨率可高达 1nm 以下。 目前常见的场发射电子枪有两种:冷场发射式(cold field emission , FE
迄今最遥远cD型星系距地约115亿光年
近日，《天体物理学报》报道了迄今发现的最遥远的cD型星系。这个命名为GOODS-S J033237.19-274608.1的星系由沈阳师范大学/加州大学圣克鲁兹分校科研人员刘凤山博士联合美、法、意、中、德的十余所科研机构的二十余位天文学家共同发现。这个星系位于GOODS-South Fi
绘制D7152441与PB2cap的结合模式图（二）
设置关键残基对象：关键残基包括：A链的K339、H357、E361、K376和F404。由于该蛋白只有一条链，在使用命令选择残基时，可以不用指定链名。按照下图顺序，将关键残基设置为res对象。2.3. 显示所需，隐藏多余在PyMOL中输入以下命令：然后，进行鼠标操作：2.4. 绘制相互作用力前面说过
毫米波隔离器最新技术进展（二）
The traditional method to tune Faraday rotation isolators is to use ferrites substantially longer than the minimum required length and adjust th
MED64应用手册：人类干细胞来源的心肌细胞应用
关键词：人类干细胞来源的心肌细胞 Human stem cell-derived cardiomyocytes (hSC-CMs) 搏动频率 beat frequency; 场电位时程 field potential duration (FPD)慢性药物作用 chronotropic drug ef
Basic－Theory－and－Use－of－GCMS（二）
For ionisation to take place at all, chemical reaction between the sample and the reagent gas must be exothermic. The grater the heat of the reaction,
SEM知识点扫盲三
9. 场发射式电子枪则比钨灯丝和六硼化镧灯丝的亮度又分别高出 10 - 100 倍，同时电子能量散布仅为 0.2 - 0.3 eV，所以目前市售的高分辨率扫描式电子显微镜都采用场发射式电子枪，其分辨率可高达 1nm 以下。 10. 场发射电子枪可细分成三种:冷场发射式(cold field emi
Intracellular－Immunofluorescent－Staining－for－Flow－Cytometry2
Table 2: Human Cytokines: Intracellular Staining Quick GuideHuman Cytokines: Intracellular Staining Quick GuideHuman CytokineCell SourceActivationIncu
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by k
GFP骨的冰冻切片实验方法
Protocol for Frozen section of GFP Bone:Fix the bone in 4% Paraformaldehyde at 4�C under constant agitation for 3 days.Decalcification in 14% EDTA sol
SYBRGreen－QPCR－in－the－ABI－7700
MaterialsROX Passive Reference Dye 25 µM, 50x (Invitrogen, Cat. No. 12223-012)SYBR Green I, "10,000x" concentrate (Molecular Probes, Cat. No. S7563)Su
蛋白质电泳技术2
Decrease toxicity of destain even more!The destain procedure can be made even less toxic by replacing the destaining solution completely with MilliQ w
显微镜的七种观察方式
一.明视野观察(Bright field BF) 明视野镜检是大家比较熟悉的一种镜检方式，广泛应用于病理、检验，用于观察被染色的切片，所有显微镜均能完成此功能。 明视野 二.暗视野观察(Dark field DF) 暗视野实际是暗场照明发。它的特点和
光学显微镜有哪些种类和使用范围
1、双光路设计 以生命科学领域来说，绝大部分的光学显微镜都是单光路设计的显微镜;有一类显微镜，称之为，体式显微镜(Stereo Microscopes / Macroscopes)，或实体显微镜，或解剖镜，是双光路设计，即模仿人眼光路，对标本获取具有立体感的正像的显微镜。光路可见下图： 那体式显
对比法测定DNA浓度
Plate assay for determination of DNA concentrationA fairly accurate, rapid assay of DNA concentration can be obtained by UV visualization of samples s
Plating－in－Top－Agar
1. Warm plates to room temperature before use. Cold plates causes the top agar to solidify irregularly. DO not warm plates to 37° as the top agar will
关于罗哌卡因的化合物简介
一、罗哌卡因的基本信息 中文名称：罗哌卡因； 中文别名：柔匹华卡因；罗哌卡因碱基； (-)-(S)-N-(2,6-二甲基苯基)-1-正丙基哌啶-2-甲酰胺； 英文名称：Ropivacaine 英文别名：RopivacaineHcl/MesylateBase；ROPIVACAINE MES
Measurement－with－the－Light－Microscope
Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule ca
脉冲电场凝胶电泳的定义
脉冲电场凝胶电泳（pulsed-field gel electrophoresis），在脉冲电场中，DNA分子的迁移方向随着电场方向的周期性变化而不断改变。