SingleChainFvAntibodiesExpressedinPlants
Methods of gene cloning and genetic engineering of immunoglobulins and transgenic plant techniques have given rise to new approaches in plant biotechnology (1). The high affinity and specificity of antibodies to different structures can be used to block regulation factors in plant cells by expression of specifically designed antibody constructs (2). Antibodies expressed in plants can also be used to influen......阅读全文
免疫荧光
Immunofluorescence Technique (Spector Lab)protocol for immunofluorescence on cells Immunofluorescence Protocol (Walter Steffen)Methanol fixationForma
Dynabeads®－Antibody－Coupling－Kit
实验概要The Dynabeads and buffers provided in this kit will enable you to covalently immobilize antibodies (or other protein ligands such as lections,
Dual－ELISPOT
实验概要We provide a protocol using an FITC-conjugated primary antibody and a biotinylated primary antibody. Those are in turn recognized by anti-FITC H
EVIDENT焕新亮相细胞年会，以奥伟登之名加速本土化战略
人工智能正以前所未有的速度重塑细胞生物学研究。从高分辨率成像到细胞行为动态分析，AI技术不仅提升了数据处理的精度与效率，同时随着AI与生物学、医学等学科的深度融合，其在细胞研究中的应用正不断突破边界，加速为生命科学研究带来全新的变革动力。今日，在珠海国际会展中心举办的中国细胞生物学学会2025年全国
EVIDENT焕新亮相细胞年会，以奥伟登之名加速本土化战略
人工智能正以前所未有的速度重塑细胞生物学研究。从高分辨率成像到细胞行为动态分析，AI技术不仅提升了数据处理的精度与效率，同时随着AI与生物学、医学等学科的深度融合，其在细胞研究中的应用正不断突破边界，加速为生命科学研究带来全新的变革动力。 今日，在珠海国际会展中心举办的中国细胞生物学学会202
叶绿素荧光技术植物逆境高温胁迫测量技术
随着全球变暖，植物高温胁迫研究受到越来越多的关注，研究手段也越来越丰富，其中包括植物荧光测量：NPQ, Fv/Fm, OJIP, and Quantum Photosynthetic Yield。本文将着重介绍如何高效、快速简便地测量这些荧光参数。非光化学淬灭（NPQ）测量非光化学淬灭（NPQ）测量
ASA抗精子抗体ELISA试剂盒使用说明（四）
7.5.3方法比较IBL抗精子抗体ELISA试剂盒与ELIAS抗精子抗体ELISA试剂盒进行比较IBL抗精子抗体ELISA试剂盒与市场上可买到的抗精子抗体ELISA试剂盒同时检测38份病人样品.线形回归结果统计如下:IBL ELISA=4.0698×ELIAS ELISA+1.0441;相关系数=0
Mapping－Protein－Distributions－on－Polytene－Chromosomes－by－Immunostaining1
INTRODUCTIONThe formidable size and structure of polytene chromosomes allowmapping of chromosomal protein distributions at very high resolution.This p
TEM－Specimen－Preparation：Preparative－Techniques－for－the－TEM
For routine transmission electron microscopy (TEM), it is generally accepted that specimens should be thin, dry and contain molecules which diffract e
便携式光合仪主要功能用于哪块呢
在控制环境因子的条件下，通过红外线气体分析仪检测二氧化碳的消耗速率来测定植物光合速率的一种仪器，简称光合仪。红外线气体分析仪法已成为目前有发展前途的光合测定手段，应用越来越普及，成为在气相环境中测定光合速率的重要方法。便携式光合仪可以测定气体CO2浓度、空气温湿度，植物叶片温度，光强，气体流量等要素
调制叶绿素荧光仪的工作原理
1983年，WALZ公司首席科学家，德国乌兹堡大学教授Ulrich Schreiber博士利用调制技术和饱和脉冲技术，设计制造了全世界第一台脉冲振幅调制（Pulse-Amplitude-Modulation，PAM）荧光仪——PAM-101/102/103。所谓调制技术，就是说用于激发荧光的测量光具
调制叶绿素荧光仪的工作原理
1983年，WALZ公司首席科学家，德国乌兹堡大学教授Ulrich Schreiber博士利用调制技术和饱和脉冲技术，设计制造了全世界第一台脉冲振幅调制（Pulse-Amplitude-Modulation，PAM）荧光仪——PAM-101/102/103。所谓调制技术，就是说用于激发荧光的测量光具
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
ImmunohistochemistyFluorescence－Protocol1
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'&
Double－immunofluore...
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies together.In order to be able to examine the co-distributi
Multicolour－3DFISH－in－vertebrate－cells4
DetectionThe choice of the detection scheme depends on several factors: (i) the number of haptens and fluorochromes used for probe labeling; (ii) the
Fluorescence－Procedures－forthe－ActinandTubulin－Cytoskeleton－inFixed－Cells1
General StrategyWe typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems,
Dynabeads®－CoImmunoprecipitation－Kit
实验概要The Dynabeads® and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface of Dynab
Dynabeads®－CoImmunoprecipitation－Kit
实验概要The Dynabeads® and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface of Dynab
Stripping－for－repro...
实验概要The following protocol provides a method of removal of antibodies from western blots.实验原理Stripping is the term used to describe the removal of pr
利用哪些叶绿素荧光参数可以监测植物热胁迫？
由于要经历漫长的炎热夏季以及未来全球变暖的预期，植物热胁迫成为科学界普遍关注的课题。已经使用许多不同类型的测量来研究植物热胁迫，包括NPQ，Fv / Fm，OJIP和量子光合产量Y(II)的叶绿素荧光测量。 本应用指南讨论了哪些协议是最有效，最快和最容易测量的。NPQ：尽管NPQ可用于测量热胁迫
Simultaneous－analysis－of－DNA－content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela
PlantScreenSC植物表型分析系统顺利安装运行
日前，由国际植物表型专家PSI公司提供、北京易科泰生态技术有限公司安装培训的植物表型分析系统PlantScreen-SC在四川成都顺利装机运行。该系统包括传送系统、成像系统、环境传感器、服务器等硬件及配套软件，适用于高70cm、宽幅50cm以内的植株。 该系统也是国内首套由公司购买使用，用
Polyclonal－Antibody－Production
实验概要Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.实验步骤Rabbit Immunization Initial
免疫细胞表面抗原分子CD家族对照表(CD1CD247)2
CD1cPositive staining (normal): subset of normal peripheral B cellsPositive staining (disease): myeloid leukemias and some B cell malignancies Negativ
“叶果两用”—FluorCam叶绿素荧光成像系统助力果树研究
日前，我们为北京农林科学院林业果树研究所安装了一套封闭式FluorCam叶绿素荧光成像系统，该系统将为果树的栽培、遗传育种、种质评价、贮藏加工等研究提供强大助力。安装培训现场，售后工程师使用老师提供的核桃叶片和核桃果实进行了测试：对正常核桃叶片（下RGB图左侧）和黄化核桃叶片（下RGB图右侧）使用叶
Fluorescence－Procedures－forthe－Actin－andTubulin－Cytoskeleton－in－Fixed－Cells
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed CellsActin: Louise CramerTubulin: Arshad DesaiGeneral StrategyWe typically wor
信号传导
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary c
Double－immunofluorescence:－sequential－protocol
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies separately.实验步骤1. Blocking and sequential incubation 1) Fi
Cardiomyocyte－immun...
实验概要The following protocol provides a method describing a suggested protocol for sarcomeric actinin, ANP, ER and ERb fluorescent staining of car