Dephosphorylation...
实验概要The method provides a protocol for removal of phosphate groups from proteins, before or after blotting. To demonstrate the specificity of an antibody for a protein in its phosphorylated state, proteins can be dephosphorylated either before SDS-PAGE or after transfer to a membrane. De-phosphorylated samples should show little or no staining compared to untreated samples.主要试剂Calf......阅读全文
Dephosphorylation－...
实验概要The method provides a protocol for removal of phosphate groups from proteins, before or after blotting. To demonstrate the specificity of an ant
Dephosphorylation－of－DNA
De-phosphorylation of DNAThe preferred method of de-phosphorylation uses the buffer system described by Pharmacia for most of their restriction endonu