GlycerolAgar(甘油琼脂)的成分和适用范围
Glycerol Agar (甘油琼脂)Peptone (蛋白胨) 5g Beef extract (酵母膏) 3gGlycerol (甘油) 20g Top water (自来水) 1000mlAgar (琼脂) 15g pH 7.0-7.2......阅读全文
Glycerol-Agar-(甘油琼脂)的成分和适用范围
Glycerol Agar (甘油琼脂)Peptone (蛋白胨) 5g Beef extract (酵母膏) 3gGlycerol (甘油) 20g Top water (自来水) 1000mlAgar (琼脂) 15g pH 7.0-7.2
Nutrient-Agar-(营养肉汁琼脂)的成分和适用范围
Nutrient Agar (营养肉汁琼脂)Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30gNaCl 5g Agar (琼脂) 15gDistilled water (蒸馏水) Adjust (调) pH to 7.0-7.2[Note]:When cultivatio
麦芽汁琼脂的成分和适用范围
Wort Agar (麦芽汁琼脂)Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then distribute th
察氏琼脂的成分和适用范围
Czapek′s Agar(察氏琼脂)Sucrose (蔗糖) 30g NaNO3 3gMgSO4.7H2O 0.5g KCl 0.5gFeSO4.7H2O 0.01g K2HPO4 1gAgar (琼脂) 13g Distilled water (蒸馏水) 1000mlpH 7.2适用范围:红霉素
黄豆饼粉琼脂的成分和适用范围
Soybean Cake Meal Agar (黄豆饼粉琼脂)10% infusion of soybean cake meal (10%黄豆饼粉浸汁) 1000mlGlucose (葡萄糖) 10g NaCl 2.5g
甘露醇琼脂的成分和适用范围
Mannitol Agar (甘露醇琼脂)Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3gMannitol (甘露醇) 25g Agar (琼脂) 15gDistilled water (蒸馏水) 1000ml
45种培养基配方(细菌培养基与植物培养基)-(一)
THE COMPOSITION OF MEDIA 培养基及成分 1. Acetobacter Medium (醋酸菌培养基) Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g CaCO3 20g Agar (琼脂) 15g
浓糖察氏琼脂的成分和适用范围
Concentrated Sucrose Czapek′s (浓糖察氏琼脂)Sucrose (蔗糖) 200g NaNO3 3gMgSO4.7H2O 0.5g KCl 0.5gFeSO4.4H2O 0.01g K2HPO4 1gAgar (琼脂) 13g Distilled water (蒸馏水)
马铃薯、葡萄糖琼脂的成分和适用范围
Potato Dextrose Agar PDA (马铃薯、葡萄糖琼脂)Potato extract (马铃薯汁) 1000ml Dextrose(glucose) (葡萄糖) 20gAgar (琼脂) 20g[Note]: Potato extract: Slice potatoes thin.
高氏合成一号琼脂的成分和适用范围
Gause′s Synthetic Agar (高氏合成一号琼脂)KNO3 1g Soluble starch(可溶性淀粉) 20gK2HPO4 0.5g MgSO4.7H2O 0.5gNaCl 0.5g FeSO4 0.01gAgar (琼脂) 20g water (水) 1000mlpH 7.2
葡萄糖、天门冬素琼脂的成分和适用范围
Glucose Asparagine (葡萄糖、天门冬素琼脂)Glucose (葡萄糖) 10g Asparagine (天门冬素) 0.5gK2HPO4 0.5g Water (水) 1000mlpH 7.2-7.4适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)
沉淀反应实验:琼脂扩散(agar-diffusion)实验
琼脂扩散实验琼脂扩散是抗原抗体在凝胶中所呈现的一种沉淀反应。抗体在含有电解质的琼脂凝胶中相遇时,便出现可见的白色沉淀线。这种沉淀线是一组抗原抗体的特异性复合物。如果凝胶中有多种不同抗原抗体存在时,便依各自扩散速度的差异,在适当部位形成独立的沉淀线,因此广泛地用于抗原成分的分析。琼脂扩散实验可根据抗原
146种培养基配方[细菌培养基和植物培养基](一)
培养基及成分 1、Acetobacter Medium (醋酸菌培养基) Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g CaCO3 20g Agar (琼脂) 15g Distilled water (蒸馏水) 1000ml Adjust (调) pH to
45种培养基配方(细菌培养基与植物培养基)-(五)
37. Clostridium Pasreurianum Synthetic Medium (巴氏梭状芽孢杆菌合成培养基) Glucose (葡萄糖) 1% MnSO4 0.001% KH2PO4 0.05% FeSO4 0.001% K2HPO4 0.05% Yeast extra
关于沉淀反应—单向琼脂扩散(simple-agar-diffusion)-的介绍
单向琼脂扩散(simple agar diffusion) 简称单扩,将特异性抗体与熔化的琼脂混合均匀,使抗体均匀分布于琼脂,然后浇制成琼脂板,再按一定要求打孔并加入抗原,使抗原向孔周自由扩散,与板中的抗体形成沉淀圈。本法为定量试验,沉淀圈的直径与抗原浓度成正比。单扩常用于血清中免疫球蛋白、AF
关于沉淀反应—双向琼脂扩散(double-agar-diffusion)--的简介
双向琼脂扩散(double agar diffusion) 简称双扩,先制备琼脂板,再按要求打孔并分别加入抗原和抗体,使两者同时在琼脂板上扩散,若两者对应且比例合适,则在抗原和抗体两孔之间形成白色沉淀线。一对相应的抗原抗体只形成一条沉淀线,因此可根据沉淀线的数目推断待测抗原液中有多少种抗原成分;
Bacterial-glycerol-stocks
To 2mls of mid-log culture or 1ml of freshly saturated culture add 1 ml(or an equal volume) of glycerol solution, mix gently, then freeze rapidly in l
146种培养基配方[细菌培养基和植物培养基](二)
22、Pine Block or Pine Sowdust Medium (松木条或松木屑培养基)(1)、Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully abs
滤纸培养基的成分和适用范围
Filter Paper Medium (滤纸培养基)(NH4)2SO4 1g KH2PO4 1gMgSO4.7H2O 0.7g NaCl 0.5gDistilled water (蒸馏水) 1000ml A strip of filter paper (滤纸条) 6ⅹ1cm (厘米)
毕赤酵母表达(pichia-pastoris-expression-)实验手册(3)
液体YPD培养基可常温保存;琼脂YPD平板在4℃可保存几个月。加入Zeocin 100ug / ml,成为YPDZ培养基,可以4℃条件下保存1~2周。2.4 YPDS + Zeocin 培养基(Yeast Extract Peptone Dextrose Medium):yeast extract
细菌保存(细菌,培养物,甘油,琼脂平板)
1.细菌保存于穿刺培养物中:一般细菌保存于穿刺培养物可以维持两年。具体方法如下:用灭菌接种针挑取分散良好的单菌落,针缓慢穿过琼脂到达瓶底,连续几次,盖上瓶盖拧紧,做好标记。室温下存放于暗处。(最好一点可以将瓶盖放松,在适当温度下培养过夜,再拧紧瓶盖并加封Parafilm膜,室温或最好在4度避光保
146种培养基配方[细菌培养基和植物培养基](三)
43、 Plasma Substitute Medium (代血浆培养基) Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5% KH2PO4 0.03% Na2HPO4.12H2O 0.14% pH 7.0-7.244、Skim Milk Medium (脱脂牛奶培养基
木屑、麸皮培养基的成分和适用范围
Sawdust, Wheat-bran Medium (木屑、麸皮培养基)Sawdust of broad leaf tree 75g Wheat bran 25gPreparation:Mix these two ingredients well . Add appropriate water a
综合马铃薯培养基的成分和适用范围
Synthetic Potato Medium (综合马铃薯培养基)20%Potato extract (马铃薯汁) 1L Glucose (葡萄糖) 20gKH2PO4 3g MgSO4.7H2O 1.5gVitanib B1 (硫胺素) Trace (微量) Agar (琼脂) 15gpH 6适
Noble-Agar-Assay
DescriptionCancer cells do not show anchorage and contact inhibition of growth. To assess the anchorage and contact independent growth of cells, noble
Preparation-of-Agar-plates
Prepare media and add 1.5 agar before autoclaving it (15g per liter). After autoclavation, cool the media in a 55 degree waterbath. Do not allow the s
Soft-Agar-Assay
Soft Agar AssayMake 0.6% media-agar mix for the bottom layer. To make 0.6% agar mix the following components (this makes 200 ml):2X DME 100 mlI
Plating-in-Top-Agar
1. Warm plates to room temperature before use. Cold plates causes the top agar to solidify irregularly. DO not warm plates to 37° as the top agar will
毕赤酵母表达(pichia-pastoris-expression-)实验手册(2)
一.毕赤酵母表达常用溶液及缓冲液的配制1.1 各种母液的配制10*YNB (含有硫酸铵、无氨基酸的13.4%酵母基础氮源培养基) 4℃保存。34g酵母基础氮源培养基(无硫酸铵)+100g硫酸铵,溶于1000ml水中,过滤除菌。500*B (0.02%生物素 Biotin) 4℃保存 保存期为1年。2
琼脂糖凝胶电泳的适用范围
琼脂糖凝胶电泳是用于分离、鉴定和提纯DNA片段的标准方法。琼脂糖是从琼脂中提取的一种多糖,具亲水性,但不带电荷,是一种很好的电泳支持物。DNA在碱性条件下(pH8.0的缓冲液)带负电荷,在电场中通过凝胶介质向正极移动,不同DNA分子片段由于分子和构型不同,在电场中的泳动速率液不同。溴化乙锭(EB)可