FateMappingAxolotlEmbryosinEarlyGastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method used to examine and explore gastrulation and determine the future fate of cells in amphibian embryos is the visualization of cell movement by application of a vital (non-toxic) dye to the region of interest on the amphibian embryo. This process of observing morphogenetic ......阅读全文
Fate Mapping Axolotl Embryos in Early Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
In vitroculture of early chick embryos
1. Use sterile technique. Prewarm Howard's Ringers in petri dish and agar/albumin culture dish to 37ºC. 2. Crack 2-day egg into large sterile pe
Development of the Heart Morphogenetic Field in the Axolotl Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Investigating the effect ofthe teratogen cyclopamine on Ambystoma mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
Developmental and phenotypic implications of transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Regulative Development in Axolotl Embryos; Splitting the Heart Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
Effect of UV Treatment on Early Development of Sea Urchin Embryos
Objective: To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage. Background: Sea Ur
Early development of primary motor neurons and somites in Zebrafish Embryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
Dorsal Lip Transplant in Amphibian Embryos
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Axolotl Protocol: Transplantation of the Gill Organ Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie