AxolotlMicrosurgery
I. Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo by carefully grabbing and pulling with fine forceps. Keep embryos in HBStcontaining antibiotics until use.2) Prepare 1 % agarose-coated operating dish, rinse with HBSt and antibiotics.3)Transfer donor and recipient embryos into operating dish containing 1......阅读全文
Axolotl Microsurgery
I. Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo
Axolotl Protocol: Transplantation of the Gill Organ Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Developmental Effects of Transplantation of Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mo
Development of the Heart Morphogenetic Field in the Axolotl Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Fate Mapping Axolotl Embryos in Early Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Regulative Development in Axolotl Embryos; Splitting the Heart Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
解密，蝾螈的再生之谜
许多蝾螈可以很容易地再生失去的肢体，但成年哺乳动物，包括人类，并不能。为什么会出现这种情况是一个科学谜团，数千年来一直吸引着自然界的观察者。 现在，缅因州巴尔港MDI生物实验室的James Godwin博士领导的一个科学家团队，通过发现促进高度再生蝾螈axolotl再生的分子信号差异，同时阻断
Developmental and phenotypic implications of transplantation
Objective In this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate
Investigating the effect ofthe teratogen cyclopamine on Ambystoma mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
Transplantation of theeye-forming region of Amphibian neurula into theflank
EMBRYO CELL AND TISSUE CULTURE TECHNIQUES Rearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solutio