PurificationofKar3MotorDomainProtein
Purification of Kar3 Motor Domain ProteinMaterialsInduced cells (2 - 5 g pellet of pET/Kar3 in BL31(DE3)pLysS host cells) (See note #1)HEM buffer =10 mM HEPES pH 7.21 mM MgCL21 mM DTT1 mM EGTAHEM + 80 mM NaClHEM + 100 mM NaClHEM + 200 mM NaCl200 mM PMSF in EtOHProtease Inhibitor Cocktail (200X) =1 microgram/mL Pepstatin1 microgram/mL Leupeptin2 micrograms/mL Aprotinin2 micrograms/mL TAME1 M DTT1 M MgCl210 m......阅读全文
Purification of Kar3 Motor Domain Protein
Purification of Kar3 Motor Domain Protein Materials Induced cells (2 - 5 g pellet of pET/Kar3 in BL31(DE3)pLysS host cells) (See note #1)
Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buff
Protein purification; actin
Protein purification; actin Overview ACTINThe most abundant muscle and non-muscle cytoskeletal protein. MW 42 kDa, 374/375 amino acids; various
Protein G Purification of Antibodies
1. Reagent and Materials(1) Hi-Trap Protein G Column (Pharmacia Biotech #17-0404-01)(2) 20 mM Sodium Phosphate Buffer, pH 7.01.084 g NaH2PO4, anhydrou
Protein Expression and Purification Protocol
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These cells must be competent. (Protocol for how to make competent cells.)a) T
Antibody Purification using Protein A, Protein G, or Protein L Agarose
实验概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should
Antibody Purification using Protein A, Protein G, or Protein L Agarose
实验概要This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants主要试剂 Protein
troponin蛋白纯化 Protein purification: troponins
Overview TROPONINS The calcium-dependent regulatory protein complex located on the thin actin filaments of muscle comprises of TnC (17.8 kDa), TnI (2
Purification of Antiserum or Ascites by Protein A/G Chromatography
1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A o
蛋白质纯化(protein purification)实用技术-3
10．非极性基团之间作用力溶质分子中的非极性基团与非极性固定相间的相互作用力（非选择性分散力或伦敦力）大小与溶质分子极性基团与流动力相中极性分子在相反方向上相互作用力的差异进行分离。因其流动相中的置换剂是极性小于水的有机溶剂（如甲醇、乙腈、四氢呋喃等），这些有机溶剂可能使许多蛋白质分子产生不可逆的变