ChemicalInductionofApoptosis
Chemical Induction of Apoptosis - 1 May 2001p53, p21WAF1, Myc, Bcl-2, Bax, Bcl-x and bak are among the proteins involved in the regulation of apoptosis. The agents and doses listed in the table below have all been used to induce apoptosis by scientists at Oncogene Research Products. Not every agent will induce apoptosis in every cell type. Depending on the agent chosen and the concentrations used, maximal induction o......阅读全文
Chemical Induction of Apoptosis
Chemical Induction of Apoptosis - 1 May 2001p53, p21WAF1, Myc, Bcl-2, Bax, Bcl-x and bak are among the proteins involved in the regulation of apoptosi
Apoptosis Induction
Introduction When studying induction of apoptosis via a cell surface molecule, it is important to first ascertain surface expression of the molecul
Induction of apoptosis through DR3 and DR4/5 Death Receptors
Apoptosis is specifically induced via signaling through a family of receptors known collectively as 'death receptors' including Fas, TNFR, DR3
Apoptosis: Miniassay
1) Aliquot 5 X 106 total cells in 5-8 ml 2% media. 2) Add 30 µl 3H-thymidine. 3) Incubate for approximately 16 hours under normal growth conditions
Chemical transformation
Chemical transformation Preparation of chemically competent cells Have the following solutions at 0-4 deg C: a) 100 mM MgCl2 b) 100 mM CaCl2-15% gl
Caspase Cascade in Apoptosis
Apoptosis, programmed cell death, is triggered by a variety of stimuli, including cell surface receptors like FAS, mitochondrial response to stress, a
Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS)
Viable cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
FACS Procedures for Apoptosis Detection
Materials: Hoechst 33258 (Sigma B-2883). stock: 10 mg/ml in dH20 (40) working dilution: 500µg/ml (50µl stock + 950µl PBS). 7-Amino-acti
Free Radical Induced Apoptosis
Oxidative stress is one factor that can trigger programmed cell death. Activated neutrophils responding to inflammatory stimulation produce reactive o