BlueNativeGelElectrophoresis
Blue Native Gel ElectrophoresisStock solutions49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT3 x Gel buffer 150 mM BisTris-HCl, 1.5 M 6-amino-caproic acid, pH 7.0 Adjust pH to 7.0 with HCl at 4°C Store at 4°C75% (w/v) Glycerol Store at 4°C10 x Cathode buffer 0.5 M Tricine, 150 mM BisTris No need to adjust pH Store at 4°C5 x Anode buffer 0.25 M BisTris-HCl, pH 7.0 Adjust pH to 7.0 ......阅读全文
Blue Native Gel Electrophoresis
Blue Native Gel ElectrophoresisStock solutions49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT3 x Gel buffer 150 m
Native gel electrophoresis（非变性电泳）
Native gel electrophoresis Under native PAGE conditions, polypeptides retain their higher-order structure and often retain enzymatic activity and inte
Agarose gel electrophoresis
General Procedure Cast a gel Place it in gel box in running buffer Load samples Run the gel Image the gel Casting Gels 0.7% ag
Gel Electrophoresis of DNA
What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab
Agarose Gel Electrophoresis
实验概要 Separating nucleic acid fragments by agarose gel electrophoresis. 实验原理 Agarose gel electrophoresis remains the most widely used technique
RNA gel electrophoresis
实验概要RNA gel electrophoresis主要试剂DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol
RNA gel electrophoresis
MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be
Alkaline agarose gel electrophoresis
Alkaline agarose gel electrophoresis (Sambrook et al., 1989) Alkaline agarose gels can be used to determine the size and quality of first and second
Polyacrylamide Gel Electrophoresis of Oligonucleotides
1. Pour and polymerize a 20% polyacrylamide gel, no Urea. 2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well. 3. Insert comb te
Agarose Gel Electrophoresis of DNA
1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose gel. 2) Cast the gel with the comb in p