AnIntegrativeProcedureforApoptosisIdentificationandMeasurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during centrifugation.(3) Before fixation with ethanol, cells should be resuspended into single cell suspension with a small amount of 1×PBS.(4) Pipetting the final cell suspension through a nylon monofilament mesh screen with Ф44 micrometer openings immediately prior to analysi......阅读全文
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Apoptosis:－Miniassay
1) Aliquot 5 X 106 total cells in 5-8 ml 2% media. 2) Add 30 µl 3H-thymidine. 3) Incubate for approximately 16 hours under normal growth conditions
Apoptosis－Induction
IntroductionWhen studying induction of apoptosis via a cell surface molecule, it is important to first ascertain surface expression of the molecule of
细胞周期的流式细胞伩检测实验方法（PI，Brdu)1
ANALYSIS OF CELL CYCLE Miriam Capri and Daniela Barbieri Dept. Biomedical Sciences, Sect. General Pathology,Via Campi, 287, University of Modena, 4110
Detection－and－Measurement－of－Radioactivity
Radioactive Decay Isotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are st
Sphingomyelin－Mass－Measurement
Protocol: Bligh & Dyer extraction 1) Pellet approximately 1 X 107 cells. 2) Resuspend pellet in 3 ml CHCl3: CH3OH (1:2) and vortex hard. 3) Add
Measurement－with－the－Light－Microscope
Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule ca
Detection－and－Measurement－of－Radioactivity
Liquid scintillation countingThe amount of kinetic energy in a beta particle differs from one decay to the next. However, each radioisotope has a typi
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials 8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go h
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials 8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go h
DNA－EXTRACTION－PROCEDURE－－GENERAL
Grow cells overnight in 500 ml broth medium. Pellet cells by centrifugation, and resuspend in 5 ml 50 mM Tris (pH 8.0), 50 mM EDTA. Freeze ce
Gramstaining－Procedure
Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should
In－Vivo－Luciferin－Imaging－Procedure
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribut
Chemical－Induction－of－Apoptosis
Chemical Induction of Apoptosis - 1 May 2001p53, p21WAF1, Myc, Bcl-2, Bax, Bcl-x and bak are among the proteins involved in the regulation of apoptosi
Caspase－Cascade－in－Apoptosis
Apoptosis, programmed cell death, is triggered by a variety of stimuli, including cell surface receptors like FAS, mitochondrial response to stress, a
James－Hardwick－CNBr－Cleavage－Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neit
Free－Radical－Induced－Apoptosis
Oxidative stress is one factor that can trigger programmed cell death. Activated neutrophils responding to inflammatory stimulation produce reactive o
FACS－Procedures－for－Apoptosis－Detection
Materials:Hoechst 33258 (Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500µg/ml (50µl stock + 950µl PBS).7-Amino-actinomycin (Sigma A-94
Granzyme－A－mediated－Apoptosis－Pathway
One mechanism used by cytotoxic T cells to kill tumor cells and virus-infected cells is the release of perforin and granzyme proteins. Perforin protei
DNA－Fragmentation－Assays－for－Apoptosis
Protocol I: Triton X-100 Lysis BufferIn 96 flat-wells plate, incubate 4x10 6 target cells (40 wells of 105 per well) with desired concentration of eff
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction 2 1.1.1 Terminology of cell death 2 1.1.2 Differences between necros
Measurement－of－Green－Fluorescent－Protein－Expression
Reagents Cells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as control. Hoechst
A－simple,－rapid－procedure－for－the－isolation－of－DNA－for－PCR
N.M. DuTeau and J.F. Leslie - Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan, KS 66506-5502 The polymerase cha
A－simple,－rapid－procedure－for－the－isolation－of－DNA－for－PCR
The polymerase chain reaction (PCR) is a method for amplifying specific segments of DNA defined by the small primers used to start the reaction. Using
Apoptosis－TUNEL－assay－(Paraffin－Sections)
Protocol for Paraffin Sections: Dewax paraffin sections: Incubate slides, 55°C, 30 min. Xylenes, 2 times, 2 min. each 100% EtOH, 2 times, 2 min.
Apoptosis－TUNEL－Assay－(frozen－sections)
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
HIV－Induced－T－Cell－Apoptosis
HIV infection is associated with immunosuppression caused by a dramatic reduction in the helper T cell population. The loss of helper T cells may be c
Journal－of－Integrative－Plant－Biology－SCI影响因子上升至2.534
据美国汤森路透(Thomson Reuters)2012年6月28日发布的2011年度期刊引证报告(JCR)，Journal of Integrative Plant Biology(JIPB)的影响因子由2010年的1.603上升到2011年的2.534，在植物科学领域的190种刊物中
Videos－for－Inline－Measurement－in－the－Pharmaceutical－Industry
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