JamesHardwickCNBrCleavageProcedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neither Immobilon nor Nylon can substitute.IMPORTANT: Wash the NC 2X for 15 min in deionized H2O after the transfer is complete. This removes any residual Tris-glycine that seems to affect the migration of the CNBr fragments during SDS-PAGE.2. Cut out the piece of......阅读全文
James－Hardwick－CNBr－Cleavage－Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neit
James－Hardwicks－angiotensin－assay－protocol
This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviousl
James－Allison：荣膺“拉斯克奖”的免疫疗法先驱
日前，美国拉斯科基金会公布了2015年拉斯克奖（Albert Lasker Award）的获奖者，包括3位科学家和一个组织。其中，临床医学奖授予了美国德州大学安德森癌症中心的James P. Allison；获奖理由是“发现并发展了一种单克隆抗体疗法，促进免疫系统对抗癌症”。 拉斯克奖是生理学
James－P.－Allison博士会获得诺贝尔奖吗？
James P.Allison博士这段时间有点忙，忙着获奖，这个奖那个奖就差诺贝尓奖了。Allison最近在湾区领了一个重大突破奖，奖金三百万，比诺贝尔奖奖金还高。Allison忙完获奖就是到处演讲，肿瘤界各种大会小会都希望他去给Keynote speech。当然不上档次的会他也不去。这几年肿瘤
聘James－M.－Tiedje为中国科学院爱因斯坦讲席教授
朱永官向James M. Tiedje颁发证书 5月7日，授予美国密歇根州立大学James M. Tiedje教授“中国科学院爱因斯坦讲席教授”仪式在中科院城市环境研究所举行，所长朱永官向其颁发证书。仪式结束后，James M. Tiedje作了题为Biology"s G
Susan－Golden和James－Golden－受聘为生物能源所客座研究员
颁发聘任证书 7月9日，美国科学院院士、加州大学圣地亚哥分校杰出教授Susan Golden和James Golden教授应邀到中国科学院青岛生物能源与过程研究所访问交流，并受聘为研究所客座研究员。所长王利生为他们颁发了聘任证书。副所长吕雪峰出席聘任仪式并主持学术报告会。 报
Angiotensin－Protein－Kinase－Assay
James Hardwick's angiotensin assay protocolThis specific procedure was developed to assay the activity of the Lck kinase expressed from a retrovir
免疫疗法大牛James－－：PD1抗体与CTLA4抗体原来如此不同
8月10日，发表在Cell杂志上题为“Distinct Cellular Mechanisms Underlie Anti-CTLA-4 and Anti-PD-1 Checkpoint Blockade”的研究中，来自德克萨斯大学MD安德森癌症中心的科学家们证实，阻断T细胞上两种不同免疫检查点
Thrombin－Cleavage－of－GSTFusion－protein
INTRODUCTIONIn many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The
Factor－Xa－Cleavage－of－MBPFusion－protein
INTRODUCTIONIn many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The
Gramstaining－Procedure
Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
DNA－EXTRACTION－PROCEDURE－－GENERAL
Grow cells overnight in 500 ml broth medium.Pellet cells by centrifugation, and resuspend in 5 ml 50 mM Tris (pH 8.0), 50 mM EDTA.Freeze cell suspensi
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
In－Vivo－Luciferin－Imaging－Procedure
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribut
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
A－simple,－rapid－procedure－for－the－isolation－of－DNA－for－PCR
The polymerase chain reaction (PCR) is a method for amplifying specific segments of DNA defined by the small primers used to start the reaction. Using
A－simple,－rapid－procedure－for－the－isolation－of－DNA－for－PCR
N.M. DuTeau and J.F. Leslie - Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan, KS 66506-5502The polymerase chain
分子克隆蛋白表达实验指南（十一）
SDS-PAGE胶样品排列： MarkerUII 37CUI’I’ Marker：低分子量蛋白marker，上样10ul UI：未诱导菌液，上样10ul。任取37C和20C中一个 I：诱导后对照，上样10ul UI’, I’代表G
Staining－Procedure－for－Flow－Cytometric－Detection－of－Human－Cyclins
Staining Procedure for Flow Cytometric Detection of Human CyclinsThis is a standard protocol used at Pharmingen for Quality Control testing of the ant
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Standard－CellTrace™－Violet－TCell－Procedure
实验概要The CellTrace™ Violet Cell Proliferation Kit provides a versatile and well-retained cell tracing reagent in a convenient and easy-to-use form. T
Keratocyte－isolation－(corneal－fibroblasts)
Corneal keratocytes (corneal fibroblasts) that this corneal layer is specialized fibroblasts residing in the stroma, representing about 85-90% of
全球首例全眼移植面临新挑战
Aaron James的蓝眼睛是自己的，棕眼睛是移植的。图片来源：Haley Ricciardi2021年，美国阿肯色州温泉城的电工Aaron James在一场高压线路电气事故中受伤，失去了左臂、左眼、下巴和鼻子。两年来，他不能吃固体食物，失去了味觉和嗅觉，也无法正常说话。2023年5月，James
世界首例全眼移植手术：创新使其成为可能
2021年，来自美国阿肯色州温泉市的一名高压线路工Aaron James在一场电气事故中受伤，他因此失去了左臂、左眼、下巴和鼻子。两年来，他不能吃固体食物，也失去了味觉和嗅觉，还不能正常说话。Aaron James现在有一只蓝眼睛和一只棕眼睛——前者是自己的，后者是移植的。图片来源：Haley Ri
新突破！人类眼球移植首次成功
外科医生进行了眼球移植手术，这在世界上尚属首次。目前还不确定接受手术者是否能够用新眼睛看到东西，但这一手术仍然朝着有朝一日使遭受严重眼部损伤的人恢复视力迈出了重要一步。 今年5月27日，美国纽约大学朗格尼医学中心的Eduardo Rodriguez和一个由140多人组成的团队，在为46岁的美国
BJ－PH/DO－推广活动火热进行中
Broadley-James品牌pH、DO电极试用活动火热进行中 美国Broadley-James公司从1976年开始设计并制造pH、DO电极及相关设备，目前已成为全球专业的pH和DO电极供应商，产品应用覆盖生物制药、食品、环保等行业。 Broadley-James公司pH电极设计独特，感应
Tunel－Procedure－in－Bovine－Embryos－牛胚胎TUNEL检测凋亡
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go highe
DIRECT－AND－SHORTTERM－PROCEDURE－FOR－HARVESTING－BONE－MARROW－CHROMOSOMES
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke
冠状病毒期间：应该先治疗一线医生和护士么？
现在是2020年3月中旬。James是一名29岁的初级医生，在伦敦一家医院工作。上周，James照顾了一个从国外回来后生病的人。该男子曾接受隔离治疗，目前病情正在好转。然而，James从此身体不适。他开始咳嗽发烧，但很快就上气不接下气了。 James因有严重急性呼吸窘迫综合征的症状而住进了自己