SphingomyelinMassMeasurement
Protocol:Bligh & Dyer extraction1) Pellet approximately 1 X 107 cells.2) Resuspend pellet in 3 ml CHCl3: CH3OH (1:2) and vortex hard.3) Add 0.8 ml H2O, vortex hard and allow solution to sit at room temperature for atleast 30 minutes.4) Pellet cellular debris by spinning at 3,000 rpm (in the tabletop centrifuge) for 5 minutes.5) Transfer solvents to a new tube without transferring any of the pelleted debris.6) Add......阅读全文
Sphingomyelin－Mass－Measurement
Protocol:Bligh & Dyer extraction1) Pellet approximately 1 X 107 cells.2) Resuspend pellet in 3 ml CHCl3: CH3OH (1:2) and vortex hard.3) Add 0.8 ml H2O
Purification－of－Demethylated－Sphingomyelin
I. Lower, chloroform phase:1) Dry on rotovapor system with house vacuum lines. It is not necessary to dry sample completely, but sufficiently to yield
Labeled－Sphingomyelin－Synthesis
Synthesis reaction:1) Mix purified DMSM with cyclohexylamine and 14CH3I at a ratio of 1/1.1/1.3 in 5 ml of methanol.2) Allow reaction to proceed at ro
Column－Purification－of－Demethylated－Sphingomyelin
Packing column:1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform.2) Place a small portion of glass wool at the base of the colu
Detection－and－Measurement－of－Radioactivity
Radioactive DecayIsotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are stable,
Detection－and－Measurement－of－Radioactivity
Liquid scintillation countingThe amount of kinetic energy in a beta particle differs from one decay to the next. However, each radioisotope has a typi
Measurement－with－the－Light－Microscope
Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule ca
质谱中手动调谐，如何理解mass－gain和mass－offset
首先，四极杆是由四根金属棒构成的，他们等距离且相互平衡，相互位置为对角位置。工作原理是在一对相对位置的四极杆上加上相同的直流电压DC和射频电压RF，在另一相对对杆上加上与上述的一对四极杆极性相反但大小相同的DC电压和相位相反但振幅相同的RF电压。通过精确设置调节这两种电压并应用在四极杆上，可以实现s
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
Measurement－of－Green－Fluorescent－Protein－Expression
ReagentsCells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as control.Hoechst 33342 s
Videos－for－Inline－Measurement－in－the－Pharmaceutical－Industry
For more information on Pharmaceutical Industry, download the Pharmaceutical brochure : Optimizing Pharmaceutical Processes
Measurement－of－Carbon－Fixation－Rates－in－Leaf－Samples
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a ran
Measurement－of－Cell－Adhesion－Under－Static－Conditions
Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell adh
Sphingomyelin－Quantitation－Postcholine－Labeling－of－HL60－Cells
Lipid Extraction1) Following the appropriate time of treatment, transfer 4.5 ml into each of two duplicate glass pyrex tubes and maintain on ice.2) Sp
SemiQuantitative－Measurement－of－Proteins－by－Dot－Blotting
Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if y
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates
AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helicobacte
Calcium－Flux－Measurement－Using－Indo1
实验概要Measure the calcium in cytosol with indo-1.主要试剂DMSO, Imonomycin, RPMI1640, PBS实验步骤1. Dissolve the contents of 1 vial (50 μg indo-1) in 50 μl dry
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Measurement－of－GFP－Expression－and－DNA－Content－in－Permeabilized－Cells
ReagentsCells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as a control.1 X PBS2% Buff
Inline－Analytical－Measurement－Systems－in－Brewing－Industry
New website for the brewing industry highlights the values of digital, in-line analytical measurement systemsThe continuous, real-time data from in-
Measurement－of－primary－endothelial－cell－permeability－to－fluxes－of－dextran..
Measurement of primary endothelial cell permeability to fluxes of dextran or albuminThe fluxes of albumin or dextran across vascular endothelial cell
精微品牌焕新－|－AMI－(Advanced－Measurement－Instruments)
精微已完成品牌整合，全面开启品牌焕新升级！ 近日，北京精微高博仪器有限公司（以下简称精微）正式启用全新品牌形象。未来，公司将聚焦产品多元化布局，深耕市场全球化战略，以更专业、更国际化、更开放的姿态，携手全球用户共赴新程。 品牌焕新·战略升级 从深耕国内到布局全球，从单一的吸附类仪器扩展到热
Sciex与Mass－Consortium签订XCMSplus软件分销协议
2015年3月30日，Sciex公司和Mass Consortium，一家质谱聚焦软件公司，签订了XCMSplus软件的独家经销商和销售权的协议。 XCMSplus软件是Mass Consortium的XCMS在线云版本，由美国斯克利普斯研究所开发的用语代谢组学数据分析的解
Vector－measurement－and－performance－tuning－of－a－terahertz－bottle－beam3
Vector characteristics of a THz bottle beamTaking advantage of the vector characterization function of the THz imaging system, the diffraction propert
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens
INTRODUCTIONFlow cytometry is frequently used to assess nucleic acid content in individual cells. Based on DNA content alone, however, cells in the qu
Vector－measurement－and－performance－tuning－of－a－terahertz－bottle－beam4
The properties of the THz bottle beam are also sensitively dependent on the base angle of the Teflon axicon. To check this point, the Teflon axi
Vector－measurement－and－performance－tuning－of－a－terahertz－bottle－beam5
Figure 8Influence of the focal length of the silicon lens to the THz bottle beam. (a) Presents the Examplitude and wrapped phase cross-sections with α
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates2
Measurement of ACTase activityNuclear magnetic resonance spect roscopy (NMR)The unique potential of NMR spectroscopy for monitoring simultaneously the
Vector－measurement－and－performance－tuning－of－a－terahertz－bottle－beam2
According to ref.4, the radius of the sharpest THz light ring can be expressed asR=ftan(arcsin(nsinα)−α),R=ftan(arcsin(nsinα)−α),(1)where α and n is
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates4
The use of a micro-titre based colorimetric assay provided a third method for the study of ACTase activity, and the most useful for large scale measur