Arapid,quantitativeandinexpensivemethodfordetectingapoptosis
ASTRACTWe describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell types. A plasmid carrying green fluorescent protein (GFP) is co-transfected with an expression vector encoding the gene of interest. Subsequently cells are stained with propidium iodide and, utilising flow cytometry, transfected, GFP-expressing single cells are detecte......阅读全文
A rapid, quantitative and inexpensive method for detecting apoptosis
ASTRACTWe describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell
A rapid, quantitative and inexpensive method for detecting apoptosis-2
Figure 1.Determination of apoptosis in transiently transfected murine [beta] tumor cells. (A) The number of apoptotic [beta]HC 13T tumor cells (% apop
ASENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS)
Viable cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
Flow Cytometry Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
An Integrative Procedure for Apoptosis Identification and Measurement-2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Yeast Cell Cycle by Flow Cytometry
Reagents Cold absolute ethanol. 0.5 M Na citrate stock (filtered), 50mM diluted stock. 10 mg/ml RNase A (Boil 10 mins, cool, filter and st
Intracellular Immunofluorescent Staining for Flow Cytometry
Introduction A modification of the basic immunofluorescent staining and flow cytometric analysis protocol can be used for the simultaneous analysis
Detection of Intracellular Antigens by Flow Cytometry
实验概要 Fix and Perm reagents are designed for use with all commercially available flow cytometers. Alignment and compensation should be performed ac
Flow Cytometry of Fibroblast Nuclei for DNA content
Materials P.I. Solution: 4 mM Na3Citrate (0.118 g/100 mL) 30 U/mL RNAseI (43 mg/100 mL) 0.1% Triton-X100 (0.1mL/100 mL) 50 µg/mL propidium iodide