E.Z.N.Z.TMMicroEluteRNADesaltingandConcentrationprotocol
实验概要This protocol is designed to clean up and concentrate RNA from various sources such as RNA isolated with RNA-solv® Reagent and other phenol involved reagents.实验步骤1. Measure the volume of sample and transfer into a new 1.5 ml microcentrifuge tube. Adjust the sample volume to 100ul with DEPC-Water and proceed to step 2.Note: if the starting samples is RNA pellet, dissolve the samples with DEPC treated water.2. ......阅读全文
Protocol－of－Northern－blot
Protocol of Northern blot质粒的转化和扩增质粒的鉴定目的基因片段的切割3.1样品双酶切(175μl水解体系)DW 115μlBuffer B(10×) 17.5μlBaM H 15μlPst I 17μlDNA(MMP-9) 16μlBSA 4.5μl37℃水浴，3h。3.2
Protocol－for－Trichl...
实验概要The efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replication)
Dot－blot－protocol
实验概要A technique for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are
Sandwich－ELISA－Protocol
实验概要The Sandwich ELISA measures the amount of antigen between two layers of antibodies (i.e. capture and detection antibody). The antigen to be mea
cDNA/AFLP－Protocol
Preparation of Para-magnetic beads from Promega cat#Z5482:a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sampleof RNA
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Histone－blotting－protocol
实验概要 Western blot detection of histone proteins. 实验步骤 The following protocol refers to the western blot detection of histone proteins derived from p
Adhesion－Assay－Protocol
Materials to be prepared beforehand:1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI)2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI)3) Lamini
Bacteria－Culture－Protocol
Bacteria Culture ProtocolBy 徐晓政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce
Yale－Immunofluorescence－Protocol
实验概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要试剂Reagents1. 384-well view plates (Aurora)2. HUVEC (pooled, L
Cytotoxicity－Assays－Protocol
Cytotoxicity Assays ProtocolCell-mediated cytotoxicity was determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted
Silver－Staining－Protocol
1x 40min - overnight 50% MeOH, 12% Acetic Acid1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min
Urea－Lysis－Protocol
Urea lysis buffer 9M Urea, 2.5mM EDTA, 2.5mM EGTA, 1% DTE, 4% CHAPS make 10ml and aliquot 10x1ml, freeze at -70°C Lysate prepara
Basic－ELISA－Protocol
实验概要 There are many different types of ELISAs, which can detect the presence of protein in serum or supernatent. One of the most common typ
Intracellular－Staining－Protocol
1. Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Colony－PCR－Protocol
1. Pull out eight glycerol stock plates from the –80oC freezer and set on bench top to thaw. Be sure to remove the foil seal before leaving the plates
DNA抽提
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction from Cell and Tissue· Mitochondria DNA Isola
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells2
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio
果蝇RNAi的实验中双链短RNA的合成（dsRNA)方法
本文来自于哈佛大学医学院果蝇RNAi筛选中心的经典实验方法，专门用于果蝇RNAi实验方法。感谢哈佛大学医学院果蝇RNAi筛选中心的支持！Primer Designed dsRNATemplate SelectionPCRIn vitro RNA TranscriptiondsRNA Purifica
ELISA
Gangliosides ELISA protocol (Contributed by pingsunjim)This protocol can be used for detection of gangliosides.Specific antibodies to gangliosides are
果蝇RNAi的实验中双链短RNA的合成（dsRNA)方法
实验概要We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Prim
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens
INTRODUCTIONFlow cytometry is frequently used to assess nucleic acid content in individual cells. Based on DNA content alone, however, cells in the qu
RNase－and－DEPC－Treatment:－Fact－or－Laboratory－Myth
Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu
Blocking－with－immunizing－peptide－protocol
实验概要The method provides a blocking with immunizing peptide protocol.实验原理Non-specific binding of an antibody to proteins other than the antigen can s
胞外基质
ECM Cell Attachment Assay (LTI)Cell Adherence Inhibition Assay (LTI)General protocol--Either monoclonal antibody or RGD peptide is added along with th
Xenograft－Tumor－Assay－Protocol
1) Determine the number of cells for injection (ie 5´106 ) to determine the number of plates thatwill require trypsinizing (usually a 100% confluent p
Native－chromatin－immunoprecipitation－protocol
实验概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes. 主要试剂10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM
Simplified－Arabidopsis－Transformation－Protocol
(Brief version for those who are familiar with the method)Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign.Our present proto
Tissue－preparation－protocol－for－ChIP
实验概要This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP). It is re