TissuepreparationprotocolforChIP
实验概要This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP). It is recommended that 30 mg of liver tissue is used for each ChIP/antibody. However, this amount may vary for other tissues. The exact amount of tissue depends upon protein abundance, antibody affinity and the efficiency of cross-linking. The ......阅读全文
Tissue－preparation－protocol－for－ChIP
实验概要This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP). It is re
Tissue－Harvest－Protocol
TISSUES TO BE PROCURED(minimally and preferably within 5-8 hours after death):1. Brain2. Liver3. Muscle4. Skin5: Others as the specific case dictatesP
Preparation－and－Staining－of－Frozen－Tissue－Sections
I. Preparation of Frozen Sections for SectioningMaterials neeed:2-methylbutane (isopentane)Liquid NitrogenDry icePeel-Away?/sup> base moldsFrozen tiss
HP－Tissue－DNA－Maxi－Protocol
实验概要The E.Z.N.A.® HP Tissue DNA Maxi Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from up to 2 grams of tissue samples.
HP－Tissue－DNA－Midi－Protocol
实验概要The E.Z.N.A.® HP Tissue DNA Midi Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from up to 500mg of tissue samples.
Example－Tissue－Bank－Protocol1
BackgroundSummarize the purpose and objectives of the tissue bank in this section.2 Eligibility to Participate in the Research Tiss
Vacuum/Spin－Protocol－for－Tissue－DNA－Extraction
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to 3
Example－Tissue－Bank－Protocol2
5.1 Record and Tissue Sample HandlingFor tissue samples arriving with identifiers, this section must clearly state whether any link is being m
E.Z.N.A.®－Protocol－for－Tissue
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to 3
Example－Tissue－Bank－Protocol3
7.2 Research with de-identified tissueThis section should describe the process for supplying researchers with tissue samples that will not hav
E.Z.N.A.®－Protocol－for－ParaffinEmbedded－Tissue
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to
细胞遗传学——原位杂交(ISH)
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization, as the name suggests, is a method of localizing,
组织学——显微解剖
Laser Capture Microdissection (LCM)Introduction to LCM (BJMU) Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections (NIH Laser Capture M
Crosslinking－chromatin－immunoprecipitation－(XChIP)－protocol
实验概要ChIP is a powerful tool that allows the specific identification of proteins or histone modifications to regions of the genome. Chromatin is isol
原位PCR
About in situ PCR (Applied Biosystems)Basic information about in situ PCR and its applications.The In Situ PCR: Amplification and Detection in a Cellu
组织学——组织制备
· Histological techniques (William H. Heidcamp)Very detailed guide to histological techniques, like fixation, dehydration, embedment and subs
RNA提取
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Procedures for Handing
细胞遗传学——比较基因组杂交(CGH)
· Comparative Genomic Hybridization (CGH) CGH is a molecular Cytogenetic method of screening a tumor for genetic changes. The alterations are
细胞组分和细胞器——染色体
Chromosomal DNA Prep : cultured cells/tissue samples (Mike A Dyer)This protocol was developed for cultured cells but should be appropriate for dissoci
免疫细胞化学
Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using peroxidase and
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Pr
转基因
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Core)Thi
Global－Expression－Profiling－of－RNA－from－Laser－Microdissected－Cells－at－...
Global expression profiling of RNA isolated from laser microdissected cells allows one to profile a specific set of cells allowing for enhanced se
单克隆抗体
· Tips and hints for the storage of antibodies (Synaptic Systems)· Purification of IgG Using Protein A- or Protein G-Agarose(KPL)·
Immunohistochemistry－Protocol－for－ParaffinEmbedded－Sections
实验概要The following is a general procedure guide for preparation and staining of formalin-fixed, paraffin-embedded tissues using a purified, unconjuga
组织学——包埋技术
Tissue Preparation for Methy Methacrylate EmbeddingMethyl Methacrylate Embedding Protocols (Energy Beam Sciences, Inc.)General protocol for plastic em
Antigen－Retrieval
实验概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortun
DNA抽提
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction from Cell and Tissue· Mitochondria DNA Isola
Antigen－Retrieval
实验概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortunat
BrdU－Labeling－Protocol
实验概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic