XenograftTumorAssayProtocol
1) Determine the number of cells for injection (ie 5´106 ) to determine the number of plates thatwill require trypsinizing (usually a 100% confluent plate of 100mm2 will yield at least 2injections at 5´106 cells/injection)2) Trypsinize the number of plates to be counted all at once3) Collect detached cells in 50 ml conical and spin for 4min at 800 rpm4) Remove sup and resuspend in 25 ml of SFM for counting5) Remove t......阅读全文
Xenograft－Tumor－Assay－Protocol
1) Determine the number of cells for injection (ie 5´106 ) to determine the number of plates thatwill require trypsinizing (usually a 100% confluent p
Xenograft－Tumor－Model－Protocol
Preparation of tumor cellsGrow cells in complete medium and exclude any contaminationWhen cells are 70-80% confluent, 3-4 hrs before harvesting, repla
Isolating－Xenograft－Tumor－Cells－for－Tissue－Culture－or－Transplantation
Isolation of T4 cells from xenograft tumors Put mouse downWash skin by dipping mice into ETOHRemove tumor and put into 5ml PBS containing Fungizone an
Migration－Assay－Protocol
Materials to be prepared beforehand:1) FBS free medium2) 10% FBS medium3) Cell migration filter insert ( Transwell®, 12mm Diameter, 12 μm Pore Size.)P
Adhesion－Assay－Protocol
Materials to be prepared beforehand:1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI)2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI)3) Lamini
Protocol－for－Aortic－Ring－Assay
ProceduresCover a 48-well plate with Matrigel (100μl/well) of and incubate for 30 min at 37°C, 5% CO2.Sacrifice the1-2 month old mice/rats (WT/mutant
alamarBlue®－Cell－Viability－Assay－Protocol
实验概要Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and c
James－Hardwicks－angiotensin－assay－protocol
This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviousl
Roche公司的RNase－Protection－Assay－(RPA)－protocol
Roche公司的RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes下载网址：http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/no1_03/PDF/p22_23.pdf还有一份
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（二）
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of
SOLID－TUMOR－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose:A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set up
Tumor－Suppressor－Arf－Inhibits－Ribosomal－Biogenesis
Cyclin-dependent kinase inhibitor-2A (CDKN2A) goes by the colloquial designation p16, which is sometimes referred to as p16(INK4). The alpha transcrip
Isolation－of－T4－cells－from－xenograft－tumors
1 Put mouse down 2 Wash skin by dipping mice into ETOH 3 Remove tumor and put into 5ml PBS containing Fungizone and Penstrep 4 Swirl around to rinse t
Isolation－and－Culture－of－Human－Brain－Tumor－Stem－Cell
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
Aspartate－Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Phosphate－Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
Protease－assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to softe
Protease－assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in
MTT－Assay
This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in
DGK－Assay
Buffers:- 2X buffer10 ml 0.5 M imidazol, pH 6.60.21 g LiCl1.25 ml 1 M MgCl21.0 ml 0.1 M EGTA, pH 6.6--> Bring volume up to 50 ml with distilled water.
Motility－Assay
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o
Chemotaxis－Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
TUNEL－assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Pectinase－assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are w
Bradford－Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
Polygalacturonase－assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
PBMC细胞的精确计数和活性分析（三）
五、使用仪器发表文章AuthorDateTitleJournalCell TypeCellometer / ApplicationsMahato, Ram INovember 2013Synthesis and Characterization of an Anti-Apoptotic Immu
RB－Tumor－Suppressor/Checkpoint－Signaling－in－response－to－DNA－damage
Cell cycle checkpoint controls at the G1 to S transition and the G2 to M transition prevent the cell cycle from progressing when DNA is damaged. The A
NAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western