UseofMicropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identified by the number on the round button on the plunger. The value is the maximum volume in microliters that can be transferred with that size pipet. Size indicator button on a Gilson P1000. Other autopipet sizes we have on hand. P1000 P200 P100 ......阅读全文
Belcher/Knight:－Electrocompetent－Cells
Electrocompetent CellsFrom Danijela Dukovski at Harvard Medical School. This protocol works well. --Julie NorvilleMaterialsDI water10% GlycerolSpecial
AbC™－AntiMouse－Bead－Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u
Experimental－Surgery2
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or wh
GST－Activity－Fluorometric－Assay
实验概要The experiment provides a simple, fluorescence-based in vitro assay for detecting the GST activity using a fluorescence plate reader. The assay
实验消毒技术
PurposeTo describe aseptic techniques used by Cell Culture specialists in handling products from and/or mammalian cells.SafetyProtect eyes, mucous mem
DNA－Extraction－from－Blood
实验概要The ChargeSwitch® gDNA Purification Kits allow rapid and efficient purification of genomic DNA from small volumes of human blood. After preparin
AbC™－AntiRat/Hamster－Bead－Kit
实验概要The AbC™ anti-Rat/Hamster Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation
AbC™－AntiRat/Hamster－Bead－Kit
实验概要The AbC™ anti-Rat/Hamster Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation
Live－imaging－with－Drosophila－tissue－culture－cells2
Materials & ReagentsDrosophila Schneider S2 cellsSchneiders Medium (GIBCO/Invitrogen), 10% fetal calf serum, Antibiotics (Sigma A5955)Depression slide
基本无菌化技术
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
Spectrophotometry——2
OPERATION OF B&L SPEC. 20The most commonly encountered spectrophotometer is one manufactured by Bausch and Lomb and known as the Spec 20. The 20 refer
Appendix－G:－Spectrophotometry——2
ABSORPTION SPECTRUM:Analysis of pigments often requires a slightly different use of a spectrophotometer. In the use of the instrument for determinatio
Crosses
Weed out the plant pot, leave only about 3 to 8 tall and vigorous plants (in a regular 5 cm pot).On an individual inflorescence, cut off all the ope
Spectrophotometry
A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the sol
Fibroblast－Cell－Systems2
Handling Precautions Normal human cells are fragile, and require special handling:Upon receipt, immediately store cryopreserved cells in liquid nitrog
Appendix－G:－Spectrophotometry
Figure G.1 Electromagnetic Radiation SpectrumFigure G.2. Schematic light transmissionFigure G.3. Use of the Spec 20A spectrophotometer or colorimeter
美国FDA将撤回2016果蔬汁作为着色剂的行业指南草案
据美国食品药品管理局（FDA）消息，近日美国FDA发布通告称，由于存在技术问题，将撤回2016果蔬汁作为着色剂的行业指南草案。 据了解，近期有评论指出，行业指南草案中的做法与现行企业中提升食品安全的操作规范不一致。美国FDA曾就果蔬汁作为着色剂，以举例的方式回答了企业的疑问。在美国，着色剂用
Adrenal－chromaffin－granule－(chromaffin－vesicle)－preparation
Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This prep is adapted from the classic paper of Smith and Winkler (Smith AD; W
AbC™－AntiMouse－Bead－Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u
Culturing－Human－Neural－Stem－Cells
实验概要Neural stem cells (NSC) are valuable resources because of their ability to differentiate into neurons and glial cells with applications in neur
普鲁兰混标试剂使用说明书
Cat No. 中文名称 Name Mw Mp Mn 规格 34037060 普鲁兰混标套装 Pullulan KIT
Hippocampal－Neuron－Cultures
实验概要The protocol provides a method of hippocampal neuron cultures.主要试剂Begin by timing the pregnant mouse at E17-E19 days of gestation. Have ready the
Microtubule－Spindowns－for－Visual－Analysis
Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live D
The－Dos－and－Donts－of－Cell－Culture
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (P
LOWRY－PROTEIN－ASSAY
The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-
Immunofluorecence－P...
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
什么是ICH？ICH的简介
ICH简介:ICH原为International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use(人用药品注册技术要求国际协调会),现已更名
PCR基本实验方法（五）
Cloning PCR ProductsT-A Cloning Strategy: Taq and other polymerases seem to have a terminal transferase activity which results in the non-templated ad
Rat－Follistatin,FS－ELISA－Kit（大鼠卵泡抑素(FS)ELISA试剂盒）使..
Rat Follistatin,FS ELISA Kit使用原理大鼠卵泡抑素(FS)ELISA试剂盒货号:FK-QZ1936规格：96T/48TThe kit was a solid phase sandwichenzyme-linked immunosorbent assay (ELISA
Pouring－Plates
1. For rich media, weigh out appropriate ingredients and place into a flask. Add water until appropriate volume. Use a flask at least 2 times larger t