UseofMicropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identified by the number on the round button on the plunger. The value is the maximum volume in microliters that can be transferred with that size pipet. Size indicator button on a Gilson P1000. Other autopipet sizes we have on hand. P1000 P200 P100 ......阅读全文
Tubulin－Basics
I. Useful Values1 mg/ml tubulin = 10 µM (assuming MW of ab-tubulin heterodimer is 100,000; in reality it is ~110,000 but almost all tubulin labs use t
Bradford－–－Protein－Determination
Bradford – Protein DeterminationIntroductionA rapid and accurate method for the estimation of protein concentration. The technique is simpler, faster
细胞培养实验的无菌化技术
The use of aseptic technique is essential for avoiding the production of infection whilst undertaking tissue culture activities.Many activities take p
Appendix－F:－Centrifugation——2
ROTOR COMPARISONSWhen separating a particle, it is convenient to be able to compare one rotor's design characteristics with those of a differing r
离心的基础知识
Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. The theoretical basis of this technique is the eff
PCR基本实验方法（一）
Recommended Reagent Concentrations:Primers: 0.2 - 1.0 uMNucleotides: 50 - 200 uM EACH dNTPDimethyl sulphoxide (DMSO): 0 - 10% (v/v)Taq polymerase: 0.5
PCR基本实验方法（一）
Recommended Reagent Concentrations:Primers: 0.2 - 1.0 uMNucleotides: 50 - 200 uM EACH dNTPDimethyl sulphoxide (DMSO): 0 - 10% (v/v)Taq polymerase: 0.5
Protocol－for－dsRNA－Synthesis
实验概要 We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends
加拿大批准乙酸钾与二乙酸钾用于部分肉禽产品
食品伙伴网讯 据加拿大卫生部消息，近日加拿大卫生部发布通告，更新防腐剂列表，批准乙酸钾与二乙酸钾用于部分肉禽产品。本次修订自10月21日生效。 加拿大卫生部于6月7日发布相关提案，并征求意见。经过评估，加拿大卫生部认为，乙酸钾与二乙酸钾用于部分肉禽产品是安全的。 部分原文报道如下：
RNA电泳实验方法
Polyacrylamide Gel Electrophoresis (PAGE)for use withRibonuclease Protection Assay (RPA):1. Making the Gel: 5% Denaturing gel for Ribonuclease Protec
PCR－clean－up
Following PCR, you often want to get rid of the PCR primers and Taq polymerase before the next step. This is necessary for sequencing PCR products or
登革热基孔肯雅热三合一核酸检测试剂盒英文使用说明
Lot-No.Ref. FR342 Expiry time: 1 year100 Tests (Ready to use PCR kit) -Only for in vitro use--Only for research use – To be used by a technical pers
新技术：InCell－Western－Assay2
III. Experimental ConsiderationsProper selection of microplates can significantly affect the results of your analysis, as each plate has its own chara
Extraction－of－25NT－RNA
实验概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation times and vo
Wholemount－staining－of－embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC.Rehydrate by slow
SYBR－Green－Quantitative－PCR－Protocol
SummaryQuantitative PCR is a method used to detect relative or absolute gene expression level. All qPCR involves the use of fluorescence to detect the
RNA－gel－electrophoresis
MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be
Centrifugation2
RELATIVE CENTRIFUGAL FORCE Figure F.2. Nomogram for R.C.F.Modern day ultracentrifuges can generate forces in excess of 300,000 times that of gravity,
Bradford－Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
Basic－Protein－Chemistry－Techniques
Coomassie Blue Stain: (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2O. 2) Add 0.5 grams of Coomassie Blue. 3) Just before use, add 50 ml acet
Phosphatasing－with－Shrimp－Alkaline－Phosphatase－(S.A.P.)
Phosphatasing with Shrimp Alkaline Phosphatase (S.A.P.)Use to prevent linearized vector plasmid from recircularizing.The phosphatase from shrimp is ea
国际纯水标准
International Organization for Standardization specification for water for laboratory use ISO 3696: 1995 This standard covers three grades of water as
液氮罐日常使用注意事项
1.容器上真空(vacuum)嘴、安全(security)阀的封条、铅封不能损坏(damage)，严禁打开真空嘴。 2.为保证容器使用(use)的安全(security)、可靠，本容器只能充装液氮。液氮罐一般可分为液氮贮存罐、液氮运输罐两种。 贮存罐主要用于室内液氮的静置贮存，不宜在工作状
RNA－gel－electrophoresis
实验概要RNA gel electrophoresis主要试剂DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol
Bacterial－cell－culture
MaterialsGlass culture tubes with metal caps and labelsGrowth medium, from media room or customizedGlass pipette tubesParafilmEquipmentVortexerFireboy
Basic－Protein－Chemistry－Techniques
实验概要Basic Protein Chemistry Techniques实验步骤Coomassie Blue Stain: (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2O. 2) Add 0.5 grams of Coomassi
Crystallization－Trials
MaterialsMilipore filter type HA 0.45 micronCulture plates (Linbro model 76-033-05)Protein in DDW or HEPES buffer (10 mg/mL)Vacuum greaseSyringe with
DNAse－posttreatment－for－nuclear－antigens
Rationale: The use of DNAse to improve nuclear antigen staining has been published long before the AR era 1, 2.DNAse treatment is currently suggested
Belcher/Knight:－Electrocompetent－Cells
Electrocompetent CellsFrom Danijela Dukovski at Harvard Medical School. This protocol works well. --Julie NorvilleMaterialsDI water10% GlycerolSpecial