ExperimentalSurgery2
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or who is to recover from an anesthestic procedure. The use of such materials under these conditions constitutes inadequate veterinary care under the Animal Welfare Act. The IACUC has established the following guidelines for the use of expired medical materials:&nb......阅读全文
Experimental－Surgery
General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery has
Experimental－Surgery2
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or wh
Experimental－Surgery3
Antibiotic GuidelinesGeneral RecommendationsSelectionIf a culture and sensitivity is not available, select antibiotics based on probable organism and
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
Experimental－Protocol－for－cDNA－Library－Construction
Experimental Protocol for cDNA Library ConstructionIdentify appropriate celltype over-expressing corresponding gene.Find out if transcription can be s
Yale－University－Policy－for－Aseptic－Amphibian－Survival－Surgery
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Apoptosis:－A－Laboratory－Manual－of－Experimental－Methods－Andrea－Cossarizza
THE CELL 1. Morphological aspects of apoptosis Walter Malorni, Stefano Fais & Carla Fiorentini 2. Cell cycle Miriam Capri & Daniela BarbieriTHE NUCLEU
Experimental－Brain－Research：双腿平衡研究新发现
2015年12月30日讯/生物谷BIOON/众所周知，用双腿走路本来就不稳定，这意味着人的中枢神经系统必须努力保持平衡。步行运动是由两种前馈控制法来控制的，在步行期间出现可能干扰行走的稳定性时身体已经做出反应，当身体对一种未知的干扰产生反应而保持直立时就会得到反馈控制。这些控制方法的精确机制目前
JAMA－Surgery：减肥术治疗2型糖尿病
近日，发表在《JAMA Surgery》的一项研究表明， Annemarie G. Hirsch博士和他的同事们研究了DiaRem得分（经过验证的分数是来自比较易得的数据），DiaRem得分可以用于预测经过减肥手术的病人是否会促使2型糖尿病治愈。这个分数可以用来预测减肥手术是否会促使短期缓解糖尿
Experimental－Neurology：如何缓解中风带来的大脑损伤？
中风患者如果其免疫细胞进入大脑，则会导致疾病的恶化。来自罗彻斯特大学医学院的研究者们目前正视图利用肺脏调节机体的免疫反应，进而缓解中风后的二次脑损伤的严重程度。 "我们越来越清楚肺脏是调节机体免疫系统的重要部位，因此能够作为治疗中风二次损伤的潜在靶点"，来自URMC的神经学家Marc Halt
基本无菌化技术
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
Principles－of－Aseptic－Technique
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
基本无菌化技术4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.All
12篇Lancet/Nature文章被撤回，这项“颠覆性”的技术，为何成为杀人工具？
2011年，使用人工合成POSS-PCU纳米复合结构体植入自体干细胞，进行了首例组织工程气管-支气管移植物的人体成功移植。 2012年8月16日，Paolo Macchiarini团队在Biomaterials（IF=14）在线发表题为“Viability and proliferation
破冰行动：提速DNA电泳速度
4、 电源电压 在低电压时，线状DNA片段的迁移速率与所加电压成正比。但是随着电场强度的增加，不同分子量的DNA片段的迁移率将以不同的幅度增长，片段越大，因场强升高引起的迁移率升高幅度也越大，因此电压增加，琼脂糖凝胶的有效分离范围将缩小。要使大于2kb的DNA片段的分辨率达到最大，所加电压
自由基快速检测分析仪应用：氧化应激与主动脉手术后...
自由基快速检测分析仪应用：氧化应激与主动脉手术后肌肉萎缩具有相关性Redoxsys自由基快速检测分析仪应用性论文Oxidative stress is associated with muscle wasting after aortic surgery（氧化应激与主动脉手术后肌肉萎缩具有相关性）R
miRNA的表达与肺鳞癌的诊断与预后
世界上每年有超过160万例肺癌新发病例，超过137万人死于肺癌。中国每年有52万例肺癌新发病例，超过45万人死于肺癌。80%肺癌是非小细胞肺癌，非小细胞肺癌中40%是鳞癌。ⅠA期肺鳞癌患者的5年存活率约为60%，而Ⅱ-Ⅳ期肺鳞癌患者的5年存活率为5% - 40%。因此，发现新的标志物并应用于早期
中科院发布期刊分区表！附一区二区期刊清单
医学CA-A CANCER JOURNAL FOR CLINICIANS1189.734NEW ENGLAND JOURNAL OF MEDICINE170.408NATURE REVIEWS DRUG DISCOVERY151.429LANCET148.362JAMA-JOURNAL OF THE
Lasers－Surg－Med：低剂量光动力学疗法可以调控肿瘤微环境
一项新的研究揭示了低剂量光动力疗法影响血管微结构的机制。通过体外共培养周细胞和内皮细胞，研究人员发现低剂量光动力疗法通过Rho、肌球蛋白轻链和局灶性粘附激酶磷酸化(MLC‐P, FAK‐P)来激活周细胞。这导致细胞骨架重构，导致周细胞更有效地收缩三维胶原凝胶。图片来源：Laser in Surg
Principles－of－Aseptic－Technique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
FGFR－INHIBITOR,－SU5402,－ON－CRANIOFACIAL－DEVELOPMENT－IN－CHICKEN－EMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
PrestoBlue™－Cell－Viability－Reagent－Protocol
实验概要PrestoBlue™ Cell Viability Reagent is a ready‐to‐use reagent for rapidly evaluating the viability and proliferation of a wide range of cell type
Separation－of－sea－urchin－blastomeres
Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos.Procedure: We extractrf the g
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辛迪思有限公司－Synthes－GmbH－对创伤外科手术器械包主动召回
强生（上海）医疗器材有限公司报告，由于螺丝刀轴杆顶端凹槽未进行加工，生产商辛迪思有限公司 Synthes GmbH 对其生产的创伤外科手术器械包Instruments for Trauma Surgery （国械备20180944号）主动召回。召回级别为三级召回。涉及产品的型号、规格及批次等详细
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牙颌面畸形（dento-maxillofacialdeformities）由患者个体内的遗传（基因）系统存在异常引起的颌骨生长发育异常所引起的颌骨体积、形态，以及上下颌骨之间及其与颅面其他骨骼之间的关系异常和随之伴发的牙合关系及口颌系统功能异常，外观则表现为颌面形态异常。以研究和诊治牙颌面畸形为