CellCultureMediaandSolutions
Antimycotic/antibiotic media:To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add:165.0 ml fetal bovine serum, heat inactivated12.0 ml 200mM (100X) L-glutamine12.0 ml antimycotic/antibiotic (100X), liquid, Gibco, Cat. No. 600-5240AGFilter sterilize through a 0.22 祄 cellulose acetate filter and store up to 2 weeks at 4 degrees C.1X Cyclosporin media: (100 ml)To 100 ml of growth media add:1.0 ml 100X Cyclospo......阅读全文
Method:－Lymphocyte－Transformation
Method: Lymphocyte Transformation May 30, 1990 Rosalie Veile Principle: Lymphocytes are transformed to establish cell lines. Mononuclear
Primary－brain－cell－isolation－and－culture
1. Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/
Human－B－cell－isolation－and－culture
B cell isolation1. Donor blood was obtained with informed consent under guidelines issued by the Medical Ethics Committee. 2. Mononuclear cell fractio
Follicular－thyroid－cell－isolation－and－culture
Thyroid epithelial cells (also called follicular cells or principal cells) are cells of thyroid gland that are responsible for the production and
Human－B－cell－isolation－and－culture
实验概要This protocol provides a general protocol for human B cell isolation and culture.实验步骤B cell isolation1. Donor blood was obtained with informed con
Isolation－and－culture－of－smooth－muscle－cell
1.Male Wistar rats of about 250g body wt were anesthetized with ether and were decapitated. 2.Kidneys and thoracic aorta were removed, rinsed with
Subculturing－Adherent－Cells
实验概要The following protocol describes a general procedure for subculturing adherent mammalian cells in culture.主要试剂1. Complete growth medium, pre-warme
细菌培养基
Preparation of LB Plate (Dr. Chastain)prepare LB plate with or without antibioticsBacterial Culture Media Recipes (WUGSC) M9 Plate Supplement (Gottsch
Fluorescence－Procedures－forthe－Actin－andTubulin－Cytoskeleton－in－Fixed－Cells
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed CellsActin: Louise CramerTubulin: Arshad DesaiGeneral StrategyWe typically wor
Human－Embryonic－Stem－(ES)－Cell－Protocols——Media－and－Reagents
Serum Free Media for human ES cells on MEFs: can last for 7-10 daysFinal ConcentrationAmount for 250ml Stock solution80% DMEM-F12200ml20% KO Serum Rep
ES－Cell－Culture－and－Manipulation3
Care and Handling of Feeder Layer Cells STO/SNL cells are a derivative of the standard STO cell line. They are transformed with a LIF producing pl
The－Dos－and－Donts－of－Cell－Culture
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (P
A－primary－cell－culture－model－of－rabbit－uroepithelium
Isolation of Epithelial Cells from Rabbit Bladders 1. Animal experiments were performed in accordance with the Animal Use and Care Committee. 2. Urina
PC12－Cell－Culture－and－Fusion
Cell CultureMaterials1. Falcon Primaria culture dishes.2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse se
Aseptic－Technique－and－Good－Cell－Culture－Practice
AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross con
ES－Cell－Culture－and－Manipulation2
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
实验概要 Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. susp
SOLID－TUMOR－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose: A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set
原代神经元培养
Protocol for the Primary Culture of Cortical and Hippocampal neurons Solutions and media required:Poly D-lysine/laminin solution - pdfDM/KY - pdfOptim
新技术：InCell－Western－Assay
In-Cell Western AssayComplete Sample Protocol Detailing the SeedingStimulation, and Detection of the HeLa CellularResponse to Epidermal Growth FactorI
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose: A: Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspec
可靠的CCCadvanced－FN1无异源耗材支持人间充质干细...（一）
可靠的CCCadvanced FN1无异源耗材支持人间充质干细胞扩增表达Reliable and Robust Animal-Component-Free hMSC-BM Expansion on Ready-to-Use Eppendorf CCCadvanced™ FN1 Motifs Surf
显微镜技术——电子显微技术
The Transmission Electron Microscope (TEM) (HEI)An explanation of how the TEM works. TEM Specimen Preparation (HEI) Serial Sectioning (Walter Steffe
Isolation－and－Culture－of－Human－Brain－Tumor－Stem－Cell
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
RNA－Isolation－From－Animal－tissue－or－cell－culture
实验概要This method is designed for most animal tissues and culture cells. For RNA isolation from fibrous tissue, follow the specialized protocol on pag
293细胞培养(cell－culture)技术
1、293细胞明显适应酸性环境,pH值在6.9～7.1时,可顺利贴壁生长, 换液时动作要轻。一般用高糖的DMEM培养基。2、传代：倒去废液，PBS洗一次（轻），用0.02%EDTA与0.25%Trypsin消化，生长良好细胞，培养瓶中轻摇,使之流遍所有细胞表面,即将其吸除或弃去消化30s，然后吸去，
Transfection－of－Mammalian－Cells－Using－Lipofectamine
Materials: Lipofectamine Basal Medium containing 10% fetal bovine serum, 1% glutamine, 1% aa Basal Medium containing 1% glutamine
RAT/MOUSE－GROWTH－HO...
实验概要This Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive quantification of Growth Hormone in rat or mouse serum, plasma, tissue
Preparation－of－Bacterial－Proteins－for－Analysis－by－2DPAGE
The following protocol has been developed for preparing soluble bacterial proteins in a form suitable for analysis by 2D-PAGE. The procedure was prin
胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routine Splitting and freezing of cells (