Aprimarycellculturemodelofrabbituroepithelium
Isolation of Epithelial Cells from Rabbit Bladders 1. Animal experiments were performed in accordance with the Animal Use and Care Committee. 2. Urinary bladders were obtained from New Zealand White rabbits (3–4 kg). 3. Typically, two rabbits were used per culture. 4. Each rabbit was euthanized with 250 mg of pentobarbital, the bladder was exposed, the ends of the bladder wer......阅读全文
A primary cell culture model of rabbit uroepithelium
Isolation of Epithelial Cells from Rabbit Bladders 1. Animal experiments were performed in accordance with the Animal Use and Care Committee. 2. Urina
Primary brain cell isolation and culture
1. Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/
Exercise 12.10 - Establishment of a Primary Culture
Exercise 12.10 - Establishment of a Primary Culture LEVEL III Materials Chick embryo (approximately 8 days old) 70% (v/v) ethanol for swa
Primary type II pneumonocyte Isolation and culture
Pulmonary alveolar type II cells carry out highly specialized functions that include the synthesis, secretion, and reutilization of surfactant, a
Organotypic raft culture of primary human keratinocytes (PHKs)
Organotypic raft culture of primary human keratinocytes (PHKs)NotesUse 106 keratinocytes per raft.Typical ProtocolTrypsinize the PHKs off the flask an
Primary Culture of Pulmonary Arterial Smooth Muscle Cells
Primary Culture of Pulmonary Arterial Smooth Muscle Cells 1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from hum
Ex Vivo Human Primary Mesenchymal Stem Cells (MSCs) Culture
Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs). MSCs produce adventitial cells in the human bone marrow microenvi
Maintenance of Cell Culture
Maintenance of Cell Culture Author: Nanci Donacki Source: Contributed by Nanci Donacki Date Added: Tue May 14 2002
Bacterial cell culture
Materials Glass culture tubes with metal caps and labels Growth medium, from media room or customized Glass pipette tubes Parafilm Equ
Immunofluorescent Localization of Tubulin
LEVEL II Materials Coverslip cultures of an appropriate monolayer cell line Phosphate buffered saline (PBS) Acetone/Methanol (absolute)