96WellSamplePreparationforAdherentCells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, etc.). In this procedure, cells are gently lifted from their culture vessel, placed into wells of a 96-well filter bottom plate, and stimulated as desired. At the end of stimulation, cell culture medium is removed from the bottom of the w......阅读全文
Coenzyme－A－Detection
实验概要The experiment provided an easy, convenient assay to measure the CoA level in a variety of biological samples. In the assay, free CoA is specif
Leave－Inaccurate－Concentrations－Behind
May 09, 2016 - Greifensee, SwitzerlandManual sample processing using flasks can introduce error into lab processes that lead to Out-of-Specificati
全谱分析——探究小分子化合物的“大工具”
样品中如此微小的小分子化合物，究竟是如何进行分析的？代谢物全谱分析，通过色谱串联质谱（HPLC-MS/MS）技术鉴定样品中小分子化合物，从而达到对其进行定性定量分析的目的。利用超高效液相色谱质谱联用技术对物质分离和鉴定，结合质谱数据库信息注释和分类，精准定性，帮助我们从整体上了解样本的性质，详细的描
CD3/－CD4－/CD8－T－cell－subset－counts
DescriptionProcedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody Procedure1. One 5 ml round bottom tube (Falco
Leaf－GUS－Assay
实验概要a protocol for Leaf GUS Assay This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs there are
Acetone－precipitation－of－protein
This procedure is suitable for recovering proteins from most aqueous solvents and from SDS containing buffers. It is not recommended for proteins diss
Peptide－competition...
实验概要The peptide competition assay/blocking procedure can be used in western blotting, IHC and ELISA. The peptide competition assay (PCA) is a reco
德国IBL－ASP－ELISA试剂盒使用说明（四）
J. PREPARATION OF DA SAMPLES FROM OTHER MATRICES a) Preparation of algal samplesThe analysis of algal samples will depend on the amount of algae (cel
DAPI－Nucleic－Acid－Stain
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Genomic－DNA－Extraction－－PureLink™
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently isolate g
NuPAGE－Gels
NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis. The gels are made up of Bis-Tris-HCl (pH 6.4) polyacrylamide and are inten
Horizon和Waters公司推出水分析的新型样品制备技术
Oasis® HLB萃取盘为环境实验室创造了更高的效率 简介：Salem, NH, 2008年11月3日—— Horizon技术公司和Waters公司推出了Oasis HLB萃取盘，一种用于EPA 8270和1694方法分析的固相萃取产品。 Horizon Technology and Wate
Immunoprecipitation
Immunoprecipitation (IP) is one of the most widely used immunochemical techniques. Immunoprecipitation followed by SDS-PAGE and immunoblotting, is rou
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
Multiple－studies－with－a－single－experiment:－The－Power－of－...（五）
Competitive Advantages Trust your quantitation! Multinotch MS3 quantitation is more accurate than other MS2 Methods The accuracy of Multinotch MS3 qua
Spectrophotometry——2
OPERATION OF B&L SPEC. 20The most commonly encountered spectrophotometer is one manufactured by Bausch and Lomb and known as the Spec 20. The 20 refer
Example－Tissue－Bank－Protocol2
5.1 Record and Tissue Sample HandlingFor tissue samples arriving with identifiers, this section must clearly state whether any link is being m
Viscosity－－Polymeriztion－of－Microtubules
LEVEL IIMaterialsTubulin (Brain extract from Exercise 9.4)GTPATPViscometerWaterbath or incubator at 37° CProcedureCompute the amount of GTP (M.W. 523)
美国实验室wetern方法
WESTERN BLOT PROTOCOLIn a Western blot, proteins that are separated on polyacrylamide gels on the basis of size are transferred to a membrane for dete
Aspartate－Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Bradford－Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
Western－Blotting－Protocol
实验概要The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in the given s
RNA－Isolation－Protocol
RNA Isolation Protocol(Revised 5-15-2003)Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)
Lipid－analysis－Week－3:－GAS－LIQUID－CHROMATOGRAPHY2
B: GLC procedureThe GLC will be prepared for your use. Please do not attempt to turn on the GLC on your own if it is off. Do not change any of the set
Immunoprecipitation
实验概要In the IP method, the protein from the cell or tissue homogenate is precipitated in an appropriate lysis buffer by means of an immune complex
Bacic－Immunoprecipitation
实验概要 Immunoprecipitation (IP) is one of the most widely used immunochemical techniques. Immunoprecipitation followed by SDS-PAGE and immunoblotting,
Assay－of－superoxide－dismutase－activity2
Cuvette holders in the sample chamber of the spectrophotometer were thermo-controlled at 25°C. For the blank test, 100 ml of 50 mM potassium phosphate
Layered－plates
General InformationThe cells being plated are sandwiched between layers of cell-free agar. The submerged cells grow into smaller colonies and many mor
Bradford－–－Protein－Determination
Bradford – Protein DeterminationIntroductionA rapid and accurate method for the estimation of protein concentration. The technique is simpler, faster