LeaveInaccurateConcentrationsBehind
May 09, 2016 - Greifensee, SwitzerlandManual sample processing using flasks can introduce error into lab processes that lead to Out-of-Specification results. A new METTLER TOLEDO article explores 10 reasons to leave error-prone manual workflows behind and create greater accuracy and efficiency in routine sample preparation.METTLER TOLEDO’s new, free download “10 Reasons to Say Goodbye to Inaccurate Concen......阅读全文
Leave－Inaccurate－Concentrations－Behind
May 09, 2016 - Greifensee, SwitzerlandManual sample processing using flasks can introduce error into lab processes that lead to Out-of-Specificati
Antibiotic－concentrations－for－E.－coli－selection
Antibiotic ConcentrationsThe following list shows recommended antibiotic concentrations for LB media or agar plates.AntibioticConcentrationAmpicillin1
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in
Automated－Genomic－DNA－Extraction
实验概要This section provides a general protocol for automated isolation of genomic DNA from 10-20 µl blood samples in a 96-well format using the Charge
Spectrophotometry——3
EXERCISE G.2 LOWRY PROTEIN ASSAYMATERIALS0.15% (w/v) sodium deoxycholate72% (w/v) trichloroacetic acid (TCA)Copper tartrate/carbonate (CTC)20% (v/v) F
Isolation－and－growth－of－mouse－primary－myoblasts
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remove them wi
Small－Scale－Yeast－Whole－Cell－Extract－for－IP
Grow 100 ml yeast cells in desired media overnight to an A600 of ~1.0 (0.6 to 1.2 works well). For growth in minimal media, 1 ml of a saturated overni
Appendix－G:－Spectrophotometry——2
ABSORPTION SPECTRUM:Analysis of pigments often requires a slightly different use of a spectrophotometer. In the use of the instrument for determinatio
LOWRY－PROTEIN－ASSAY
The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-
Revised－protocol－for－establishing－a－mammary－cell－line－from－tumors
Revised protocol for establishing a mammary cell line from tumorsDrench mouse in 70% ethanol, pin and dissect openSterile dissection of tumor- leave s
Neutralizing－Bioassay－Protocols
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
Automated－Extraction－－Normalized－DNA－Buccal－Kit
实验概要This section provides a general protocol for automated isolation of genomic DNA from human buccal cell swabs in a 96-well format using the Charg
Trypsinizing－cells
There are many procedures with which to trypsinize cells. All include washing the cell monolayer with TD, or in rare cases, with VE. This removes seru
One－step－miniprep－method－for－the－isolation－of－plasmid－DNA
plasmid miniprepAll ''miniprep'' methods reported so far for the isolation of plasmid DNA involve multiple pipetting, extraction, cent
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
实验概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)
SQ－Blood－Mini－Protocol－for－50ul－Clotted－Blood
实验概要This protocol is designed for isolating genomic DNA from 0.5-1 million cultured cells. For larger or smaller amounts of starting cell numbers,
Pheromone－Halo－Assay
-Use sterile technique and sterile solutions throughout this method.-1. Grow a starter culture at 30 C with shaking (250 rpm) until it reaches saturat
Immunofluorescence－Whole－Mount
Objective:Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically
Fluorescent－Nucleoside－Triphosphates－for－SingleMolecule－Enzymology4
3.2.1 LabelingThis method requires the starting material 3′-amino-3′-deoxyATP (15).1. Activate 7-diethylaminocoumarin-3-carboxylic acid (16.4 mg,
COMPARISION－OF－DIFFERENT－PROTEIN－DETERMINATION－METHODS
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions-InterferencesA
体外荧光法检测核内体早期动力学5
Leave tubes on ice and repeat Steps 13–15 for the next 6–12 plates of cells.When all cells are collected, centrifuge all tubes at 250g for 5 min at 4
Hippocampal－Neuron－Cultures
实验概要The protocol provides a method of hippocampal neuron cultures.主要试剂Begin by timing the pregnant mouse at E17-E19 days of gestation. Have ready the
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens2
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 µg/mL of 7-AAD. Incubatefor 20 min a
Sectioning－stained－embryos.
The protocols for plastic and wax sections as used by the Vize lab. These protocols work, but they have not been optimized. If anyone has better proto
Complete－PCR－Guide
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
Dual－and－TripleCo...－（二）
主要试剂1. Blocking reagent after coating: 10% fetal calf serum (FCS) in phosphate-buffered saline (PBS), pH 7.4.2. Cells suspended in complete culture me
Yeast－Cell－Cycle－by－Flow－Cytometry
ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4
A－study－of－Fetal－Alcohol－Syndrome
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d