96WellSamplePreparationforAdherentCells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, etc.). In this procedure, cells are gently lifted from their culture vessel, placed into wells of a 96-well filter bottom plate, and stimulated as desired. At the end of stimulation, cell culture medium is removed from the bottom of the w......阅读全文
CORE－SAMPLE－PCR
A method to re-PCR unique bands from products of mixed sizeContentsINTRODUCTIONPROTOCOLCOMMENTSINTRODUCTIONThe products of a PCR reaction - especially
Sample－preparation－(analytical－gels)
Sample preparation and solubilization are crucial factors for the overall performance of the 2-D PAGE technique. Protein complexes and aggregates shou
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
SC30－Sample－delivery－and－cleaning－unit－多份样品自动化
了解详情SC30 进样及清洗单元SC30 是具有 30 个样品位的自动进样器。 它利用空气压力执行进样，利用两种不同的溶剂进行自动冲洗，并利用干燥泵进行彻底干燥。 无污染回收残留样品。
CORE－SAMPLE－PCR:－A－method－to－rePCR－unique－bands－from－products－of－mixed－s
INTRODUCTIONThe products of a PCR reaction - especially when this is done on eukaryotic genomic DNA, and when using degenerate primers - often contain
双向电泳样品缓冲液(sample－buffer)选用的基本原则
1、蛋白质样品的特征：复杂，含盐或其他污染物易被蛋白酶降解动态范围宽（>X106）溶解性不均一（疏水性/亲水性）分子量、等电点的范围宽（10-500KDa，pH2-14）2、样品分离的基本要求：高灵敏度和分辨率宽动态范围小样品体积高通量合适的温度和pH避免蛋白酶降解适合与高灵敏度的质谱联用尽量少的操
瑞士万通889－IC－Sample－Center获Pittcon2011撰稿人提名
889 IC Sample Center是一款功能强大的高通量自动样品处理器，是应对现代化分析实验室挑战的优选设备，进样使用x-y-z原则和高分辨剂量系统。889 IC Sample Center的双针系统，允许分析时使用加盖或者带隔片的玻璃容器。自动进样通过一个PEEK-in
GST－Activity－Fluorometric－Assay
实验概要The experiment provides a simple, fluorescence-based in vitro assay for detecting the GST activity using a fluorescence plate reader. The assay
DNA－Extraction－from－Blood
实验概要The ChargeSwitch® gDNA Purification Kits allow rapid and efficient purification of genomic DNA from small volumes of human blood. After preparin
如何分析real－time－PCR的数据结果
学习做Realtime PCR, 实验结果是出来了,得到的内参、目的基因的Ct 值在15-25 之间,熔融曲线基本上也是单峰,但不太会处理这个结果,对着数据发愁.不同的处理方法得到不同的结果.做的是相对定量,SYBR Green, 选用2^（-△ △ Ct）法内参基因：对照组1、对照组2、对照组3实
如何分析real－time－PCR的数据结果
学习做Realtime PCR, 实验结果是出来了，得到的内参、目的基因的Ct 值在15-25 之间，熔融曲线基本上也是单峰，但不太会处理这个结果，对着数据发愁。。。不同的处理方法得到不同的结果。。。做的是相对定量，SYBR Green, 选用2^（-△ △ Ct）法内参基因：对照组1、对照组2
Bradford－Protein－Concentration－Assay
Bradford Protein Concentration Assayversion 01/07/2001Abbreviations:mcg = microgramsmcL = microlitersBSA = bovine serum albuminO.D. = optical densityd
教您德安特精密天平线性校准方法
本方法适用于德安特精密天平及西特精密天平进行校准须使用F1级以上标准砝码，一般使用者请谨慎操作。1． 开机并归零，2． 且热机20分钟 3． 按住“TARE/POWER”键不放 4． 按住“SAMPLE（MODE）”键不放 5． 放开“TARE/POWER”键 6． 再按住“TARE/POWER”键
Quantitative－Determination－of－Peptides－by－Sulfhydryl－(SH)－Groups
Quantitative Determination of Peptides by Sulfhydryl (-SH) GroupsAuthor: David Van Horn, Greg BulajSource: Contributed by David Van Horn, Dept. of Che
Detection－and－Measurement－of－Radioactivity
Liquid scintillation countingThe amount of kinetic energy in a beta particle differs from one decay to the next. However, each radioisotope has a typi
美国西特分析天平校准方法及校正方法
1.归零后，先按“扣重”再按“功能”显“FOUT”2.按“功能”键显“*G”（如400G按“扣重”选择200G）放相应砝码3.按“功能”显“ACAL”待显砝码值，校正电子分析天平注：FULL SCALE 满量程COURER DCEIGHTS 半量程UNSRAN 恢复出厂ESCAPE 退出美国西特分析
Whole－mount－fluores...
实验概要The method provides a protocol for whole mount fluorescent immunohistochemistry. The advantage of using fluorescence to stain whole mount sectio
mRNA－Amplification－with－T7－RNA－Polymerase
MaterialsMessageAmp II aRNA Amplication Kit (Cat #1751, Ambion)100% EthanolRNA samples (e.g. 1 µg RNA per sample)ProcedureReverse transcriptionVerify
Chick－or－Mouse－embr...
实验概要The following procedure describes the procedure for whole mount staining of chick or mouse embryo’s. A similar procedure could be used for sta
Bovine－Ig(Total－Immunoglobulin)－ELISA－Kit使用说明（二）
Note: Samples to be used within 5 days can be stored at 4°C, besides that, samples must be stored at -20°C (assay ≤1 month) or -80°C(assay≤2 month
Genomic－DNA－Extraction－－Phenol－|－Chloroform
实验概要This section provides a general protocol for genomic DNA extraction using phenol and chloroform.主要试剂1. Glycogen (20 μg/μL)2. 7.5 M NH4
Leaf－GUS－Assay
一、实验试剂 GUS Buffer (500 ml) 2.0478 g Na2HPO4 1.2688 g NaH2PO4 (=50 mM NaPi pH7.0) 10 ml 0.5 M EDTA (=10 mM) 0.5 g Triton X-100 0.5 g N-L
The－Effects－of－Ultraviolet－Light－on－the－Fertilization
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on bot
Appendix－G:－Spectrophotometry——2
ABSORPTION SPECTRUM:Analysis of pigments often requires a slightly different use of a spectrophotometer. In the use of the instrument for determinatio
蛋白质和多肽MALDIMS分析方法
ABSTRACTFor MALDI-TOF-MS analysis of proteins and peptides, samples are cocrystallized with an excess of organic matrix that absorbs at a specific wav
DAPI－Counterstaining－Protocols
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding