WIKI资讯
WIKI资讯
Preparation of tumor cellsGrow cells in complete medium and exclude any contaminationWhen cells are 70-80% confluent, 3-4 hrs before harvesting, replace medium with fresh medium to remove dead and detached cells.Remove medium and wash cells with PBS. Add a minimum amount of trypsin-EDTA. Disperse cells and add complete medium (10:1 to 5:1). Centrifuge immediately at or below 1500 rpm for 2-5 min and wash twice with P......阅读全文
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of