HighResolutionAgaroseGelElectrophoresis
实验概要Agarose gel electrophoresis remains the most widely used technique for separating nucleic acid fragments due to its ease of use, non-toxicity, and broad separation range. By varying agarose concentration, gel pore size can be controlled to separate nucleic acid molecules in a wide range of sizes. The migration of nucleic acids in agarose gels is affected by the choice of buffer......阅读全文
琼脂糖凝胶电泳（agarose－gel－electrophoresis）检测DNA
原理： 琼脂糖是从海藻中提取出来的一种线状高聚物，可作为电泳支持物，适用于分离大小范围在0.2-50kb的DNA片段。DNA分子的迁移率与分子量的对数值成反比关系。观察其迁移距离，与标准DNA片段进行对照，就可获知该样品分子量大小。在质粒抽提过程中，由于各种因素的影响，使质粒DNA呈现超螺旋的共
DNA片段的琼脂糖凝胶电泳（agarose－gel－electrophoresis）
【原 理】琼脂糖凝胶电泳是重组DNA研究中常用的技术，可用于分离，鉴定和纯化DNA片段。不同大小、不同形状和不同构象的DNA分子在相同的电泳条件下（如凝胶浓度、电流、电压、缓冲液等），有不同的迁移率，所以可通过电泳使其分离。凝胶中的DNA可与荧光染料溴化乙锭（EB）结合，在紫外灯下可看到荧光条带，籍
质粒DNA的琼脂糖凝胶电泳（agarose－gel－electrophoresis）
带电荷的物质在电场中的趋向运动称为电泳。电泳的种类多，应用非常广泛，它已成为分子生物学技术中分离生物大分子的重要手段。琼脂糖凝胶电泳由于其操作简单、快速、灵敏等优点，已成为分离和鉴定核酸的常用方法。实验目的：掌握琼脂糖凝胶电泳的原理，学习琼脂糖凝胶电泳的操作。实验材料：质粒DNA、BAC、植物总DN
血清蛋白琼脂糖凝胶电泳(agarose－gel－electrophoresis)（二）
【操作】1、预染血清血清0.2ml中加苏丹黑染色液0.2ml，混合置37℃水浴中染色30分钟，离心（2000转/分）约5分钟。以除去悬浮于血清中染料沉渣。2、制备琼脂糖凝胶板将已配制好的0.5%琼脂糖凝胶于沸水浴中加热融化，用吸管吸取凝胶溶液浇注在载玻片上，约3 ml。静置半小时后凝固（天热时需延长
电泳时的DNA分子量Marker和Agarose浓度的选择
电泳时的DNA分子量Marker和Agarose浓度的选择DNA片段长度Agarose浓度 使用Marker种类200 bp以下3%以上 фX174-Hae Ⅲ digest DNA Marker фX174-Hinc Ⅱ digest DNA Marker DL500™DNA Marker
血清蛋白琼脂糖凝胶电泳(agarose－gel－electrophoresis)（一）
【原理】琼脂糖（agarose）是经过挑选，以质地较纯的琼脂（agar）作为原料而制成的。琼脂在化学上是由琼脂糖和琼脂胶组成的复合物。琼脂胶是一含有硫酸根和羟基的多糖，它具有离子交换性质，这种性质会给电泳及凝胶过滤以不良的影响。琼脂糖是直链多糖，它由D－半乳糖和3,6－脱水－L－半乳糖的残基交替排列
DNA电泳
DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA from Acrylamide Gels DNA Marker
DNA－Electrophoresis
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. DNA is a negatively
RNA－Electrophoresis
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th
Gel－Electrophoresis－of－DNA
What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab
重组DNA的分离、克隆与测序实验手册2
C. Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restrict
Bacic－Immunoprecipitation
实验概要 Immunoprecipitation (IP) is one of the most widely used immunochemical techniques. Immunoprecipitation followed by SDS-PAGE and immunoblot
Immunoprecipitation
实验概要In the IP method, the protein from the cell or tissue homogenate is precipitated in an appropriate lysis buffer by means of an immune complex
Immunoprecipitation
Immunoprecipitation (IP) is one of the most widely used immunochemical techniques. Immunoprecipitation followed by SDS-PAGE and immunoblotting, is rou
ImmuneChem产品在蛋白质翻译后生物修饰最新动向
最新Nature刊登的科技文献指出： 通过比较Sigma,SantaCruz的同类产品， 只有ImmuneChem的抗生物素抗体Agarose填料达到PTM蛋白质组学质谱分析应用的质量。蛋白质翻译后生物修饰是新的PTM研究领域。 抗生物素抗体Agarose填料也是非破坏性（Non-inv
PLAQUE－ASSAYS－FOR－ADENOVIRUS－TITRATION
-Set up 60 mm dishes of P11 cells to be 100 confluent at time of infection. -Remove medium from dishes, add 0.2 to 0.5 ml virus and adsorb for 30 – 60
Top－10－Fun－Facts－for－DNA－Electrophoresis
Did you know:When preparing agarose for electrophoresis, it is best to sprinkle the agarose into room-temperature buffer, swirl, and let sit at least
DNA抽提
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction from Cell and Tissue· Mitochondria DNA Isola
蛋白质翻译后修饰的验证问题
Why are proteins, detected by mass spectrometry, not validated by site-specific antibodies?The modified motif could be detected by mass spectrometry (
PCR实验指导与常见问题分析5
MgCl2 concentrationRelationship between MgCl2 and dNTP concentrationdNTP concentrations of about 200µM each are usually recommended for the Taq polyme
Purification－of－Antiserum－or－Ascites－by－Protein－A/G－Chromatography
1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A o
Construction－of－BAC－Libraries：Megabase－DNA－Isolation
Megabase DNA IsolationMegabase-size DNA isolation from plantsTo construct large insert DNA libraries in BAC and YAC vectors, methods must be developed
Standard－Operating－Procedures－for－T1Phage－Testing－Assay
I. Introduction:This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard
对比法测定DNA浓度
Plate assay for determination of DNA concentrationA fairly accurate, rapid assay of DNA concentration can be obtained by UV visualization of samples s
Slice－and－Explant－Culture－Protocols－–－Hevner－lab－2002
for axon tracing & cell culture studies in vitro using embyros age E11.5 – E16.5modified from Rubenstein lab and Price lab protocols1. Setupa. 2 hr be
RNA电泳
RNA Gel (Crawford Lab)Gel Electrophoresis of RNA (Beverly Faulkner-Jones)great tips on RNA gel electrophoresis.Northern Gel and TransferUsing glyoxal
RNA电泳
· RNA Gel (Crawford Lab)· Gel Electrophoresis of RNA (Beverly Faulkner-Jones)great tips on RNA gel electrophoresis. · Northern
Construction－of－BAC－Libraries：Construction－of－a－BAC－library
Once high molecular weight (HMW) DNA has been prepared it must somehow be fragmented and DNA in the desired size range isolated. In general, as the de
Basic－procedures－for－bacteria－culture1
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
DNA的酶学操作
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge